logging in or signing up Getting down to the phosphorylated ‘ nuts and griffindoc Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 14 Category: Science & Tech.. License: All Rights Reserved Like it (0) Dislike it (0) Added: October 26, 2010 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Getting down to the phosphorylated ‘ nuts and bolts ‘ of spindle check point signalling : Getting down to the phosphorylated ‘ nuts and bolts ‘ of spindle check point signalling Dr. Anand R Trends in Biochemical sciences, 35; 2010 Cell cycle and Checkpoints : Cell cycle and Checkpoints Checkpoints - regulated transition points where progression to next phase be arrested by negative signals, incase of defect. Slide 4: SPINDLE ASSEMBLY CHECKPOINT Pathway monitoring the fidelity of chromosome segregation in Mitosis Active in prometaphase; prevents precocious separation of sister chromatids Delays anaphase onset until chromosome biorientation KINETOCHORE : KINETOCHORE MECHANISM OF CHECKPOINT ACTION : MECHANISM OF CHECKPOINT ACTION Unattached kinetochore activates SAC : Unattached kinetochore activates SAC Slide 14: Generation of MCC 1 2 ??? APC/Cyclosomemachine designed to destroy!! : APC/Cyclosomemachine designed to destroy!! a cullin–RING-finger ubiquitin ligase Multisubunit complex; MW 1.5 MDa the cullin domain of Apc2 is associated with the RING-finger domain of Apc11 2 large domains- platform; arc lamp 2 coactivators – Cdc20; Cdh1 Functioning of APC/Cyclosome : Functioning of APC/Cyclosome Models for APC/C inhibition : Models for APC/C inhibition SAC Kinases : SAC Kinases SILENCING OF CHECKPOINT : SILENCING OF CHECKPOINT PROTEIN STRIPPING : PROTEIN STRIPPING PP1 induced inactivation : PP1 induced inactivation Slide 29: Inactivation by p31comet Energy dependent complex dissociation Slide 30: Turning off kinases Feedback circuit at SAC : Feedback circuit at SAC Significance : Significance Aneuploidy – discrepancies in chromosome number Tumorigenesis Take home….. : Take home….. Even a single unattached kinetochore could generate ‘wait anaphase’ signal APC activation needed for both progression to anaphase and exit from mitosis Cdc 20- activator of APC MCC – effector of SAC SAC mode of action targets Cdc 20 You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
Getting down to the phosphorylated ‘ nuts and griffindoc Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 14 Category: Science & Tech.. License: All Rights Reserved Like it (0) Dislike it (0) Added: October 26, 2010 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Getting down to the phosphorylated ‘ nuts and bolts ‘ of spindle check point signalling : Getting down to the phosphorylated ‘ nuts and bolts ‘ of spindle check point signalling Dr. Anand R Trends in Biochemical sciences, 35; 2010 Cell cycle and Checkpoints : Cell cycle and Checkpoints Checkpoints - regulated transition points where progression to next phase be arrested by negative signals, incase of defect. Slide 4: SPINDLE ASSEMBLY CHECKPOINT Pathway monitoring the fidelity of chromosome segregation in Mitosis Active in prometaphase; prevents precocious separation of sister chromatids Delays anaphase onset until chromosome biorientation KINETOCHORE : KINETOCHORE MECHANISM OF CHECKPOINT ACTION : MECHANISM OF CHECKPOINT ACTION Unattached kinetochore activates SAC : Unattached kinetochore activates SAC Slide 14: Generation of MCC 1 2 ??? APC/Cyclosomemachine designed to destroy!! : APC/Cyclosomemachine designed to destroy!! a cullin–RING-finger ubiquitin ligase Multisubunit complex; MW 1.5 MDa the cullin domain of Apc2 is associated with the RING-finger domain of Apc11 2 large domains- platform; arc lamp 2 coactivators – Cdc20; Cdh1 Functioning of APC/Cyclosome : Functioning of APC/Cyclosome Models for APC/C inhibition : Models for APC/C inhibition SAC Kinases : SAC Kinases SILENCING OF CHECKPOINT : SILENCING OF CHECKPOINT PROTEIN STRIPPING : PROTEIN STRIPPING PP1 induced inactivation : PP1 induced inactivation Slide 29: Inactivation by p31comet Energy dependent complex dissociation Slide 30: Turning off kinases Feedback circuit at SAC : Feedback circuit at SAC Significance : Significance Aneuploidy – discrepancies in chromosome number Tumorigenesis Take home….. : Take home….. Even a single unattached kinetochore could generate ‘wait anaphase’ signal APC activation needed for both progression to anaphase and exit from mitosis Cdc 20- activator of APC MCC – effector of SAC SAC mode of action targets Cdc 20