CPCSEA GUIDLINES IN PHARMACOLOGY

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WELCOME:

WELCOME

CPCSEA GUIDELINES FOR LABORATORY ANIMAL FACILITY:

CPCSEA GUIDELINES FOR LABORATORY ANIMAL FACILITY RASHIKH P K DEPT OF PHARMACOLOGY

FOOD:

FOOD Animals should be fed palatable, non contaminated,and nutritionally adequate food Feeders should allow easy access to food Food should be available in amounts sufficient to ensure normal growth in immature animals Laboratory animal diets should not be manufactured or stored in facilities used for farm feeds or any product containing additives P recaution should be taken if perishable items are fed Diet should be free from heavy metals,toxins

WATER:

WATER Animals should have continous access to fresh,potable , uncontaminated drinking water Watering devices should be examined routinely to ensure their proper operation Periodic monitoring of microbial contamination in water is necessary

WASTE DISPOSAL:

WASTE DISPOSAL Waste should be removed regularly& frequently All waste should be collected and disposed off in a safe and sanitary manner The most preferred method of waste disposal is incineration Waste-can containing animal tissues,carcasses , hazardous waste should be lined with leak- proof, disposal liners. Waste storage area should be separated from other storage area Hazardous waste should be rendered safe by sterlisation or other methods

EMERGENCY ,WEEKEND & HOLIDAY CARE:

EMERGENCY ,WEEKEND & HOLIDAY CARE Animals should be cared by qualified personnel every day Posting emergency procedures, names, telephone numbers in animal facilities PEST CONTROL Programs are designed to prevent, control the pest

RECORD KEEPING:

RECORD KEEPING Animal house plans Animal house staff record Health record of staff /animals All SOPs relevant to the animals Breeding, stock,purchase and sales records Minutes of institute animals ethics committee meetings Records of experiments conducted with no; of animals used Death record Clinical records of sick animals Water analysis report Training record of staff involved in animal activities

STANDARD OPERATING PROCEDURES:

STANDARD OPERATING PROCEDURES Name of the author Title of the SOP Date of preparation Reference of previopus SOP on the same subject and date. Objectives Detailed information of the information used The name of the manufacturer of the reagent & methodology of the analysis Normal value of all parameters Hazard identification & risk assessment

PERSONNEL AND TRAINING:

PERSONNEL AND TRAINING Selection of Animal facility and staff Regular medical check up for workers Workers don’t eat,drink or smoke in animal room Training on hygeine,disinfection & sterilization must be given.

TRANSPORT OF LABORATORY ANIMALS:

TRANSPORT OF LABORATORY ANIMALS Mode of transport Containers Animal density in cages Food and water Protection from transit infection Injuries and stress.

ANESTHESIA & EUTHANASIA:

ANESTHESIA & EUTHANASIA painful procedure should be carried under anesthesia only. anesthesia is given for full duration animal is prepared for anesthesia by keeping them fasting overnight and by using pre anesthetics. EUTHANASIA : - events where an or for animal is required to sacrificed on termination of an experiment or for other ethical reasons.

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