Possibility of using ISSR Marker

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Possibility of using ISSR Marker for the development of drought tolerant genotypes in chickpea

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PP 55 Possibility of using ISSR Marker for the development of drought tolerant genotypes in chickpea Geetika Mehta Geeta Deviand Sonali Sangwan 1 Department of Plant Breeding Genetics CCSHAU Hisar Haryana 1 Department of MBBB CCSHAU Hisar Haryana Email: drgeetika1326gmail.com Chickpea Cicer arietinum Linnaeus a member of family Fabaceae is an ancient self pollinated leguminous crop. It is ranked 3rd after common bean Phaseolus vulgaris L. and pea Pisum sativum L. and is mainly concentrated in semi-arid environments. Drought is the most important constraint to yield in chickpea accounting for about 50 yield reduction globally. Therefore it is essential to develop varieties which can make use of available water resources and produce maximum yield. Most of the physiological traits associated with drought tolerance are quantitative in nature. An important research strategy that has been widely used over the past two decades to deal with such complexity is to use molecular markers to identify quantitative trait loci Qtls and their applications in marker-assisted selection MAS programmes. The present study on chickpea was conducted in the dryland area of CCS Haryana Agricultural University Hisar for two consecutive years to screen 21 genotypes for rainfed conditions. In addition to morphological and physiological traits recorded on these genotypes their ISSR DNA profile was also analyzed. Lots of genetic diversity was recorded at DNA level among all the genotypes. NTSYS pc2.0 was used to cluster them. One of the ISSR markers UBS841 was found to be associated with deeper root length in chickpea. Morphological physiological and molecular observations in combination can be used to identify high yielding plants in chickpea for rainfed conditions drought. The ISSR marker thus identified can also be successfully employed in MAS programmes for drought tolerance.

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