Presentation Transcript
The effect of temperature on nuclear translocation of CLIC channels in Caenorhabditis elegans :The effect of temperature on nuclear translocation of CLIC channels in Caenorhabditis elegans
Significance: :Significance: exl-1 seems to translocate to the nuclei after heat shock
resembles the vertebrate CLIC4, which induces apoptosis when it translocates to the nucleus under stress
C. elegans is potentially a model organism for research in stress-induced apoptosis
Procedure: :Procedure: C. elegans was exposed to various temperatures for 2 and 3 hours: one cold shock (8°C), the control (20°C), and four heat shock (30°C, 35°C, 37.5°C, 40°C)
I picked the worms onto agarose pads layered on glass slides
I viewed them under 5X with the fluorescent microscope and took pictures with QCapture
I calculated the percentage of worms with clusters of translocated CLIC channels
8°C :8°C 2 hrs 3 hrs
20°C :20°C
30°C :30°C 2 hrs 3 hrs
35°C :35°C 2 hrs 3 hrs
37.5°C :37.5°C 3 hrs 2 hrs
40°C :40°C 2 hrs 3 hrs
Things that went wrong: :Things that went wrong: Worms at higher temperatures appear larger (and probably are older) because their aging is affected by temperature
Some worms seemed to be very young, and not very clear
Worms died after 37.5°C
Pictures could be clearer if there was a way to stop worms from moving without killing them
Further experimentation: :Further experimentation: Confirm that the clumps of exl-1 are in nuclei
Experiment with more temperatures between 30°C and 37.5°C
See if SMA9 mutants behave the same way
See if there is anything else that would prevent or enhance CLIC nuclear translocation
THANK YOU! :THANK YOU!