Sickle cell anemia

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Sickle cell anemia:

Sickle cell anemia

B-hemoglobin gene:

B-hemoglobin gene

The Gene Associated with Sickle Cell Anemia:

The Gene Associated with Sickle Cell Anemia Official Gene Symbol: HBB Name of Gene Product: hemoglobin, beta Alternate Name of Gene Product: beta globin Locus: 11p15.5 - The HBB gene is found in region 15.5 on the short (p) arm of human chromosome 11. Gene Structure: The normal allelic variant for this gene is 1600 base pairs ( bp ) long and contains three exons. mRNA: The intron-free mRNA transcript for the HBB gene is 626 base pairs long. See the NCBI sequence record NM_000518 to access the mRNA sequence data. Coding Sequence (CDS): 444 base pairs within the mRNA code for the amino acid sequence of the gene's protein product. Protein Size: The HBB protein is 146 amino acids long and has a molecular weight of 15,867 Da. See the annotated HBB protein sequence record in Swiss-Prot. Protein Function: Hemoglobin molecules, which reside in red blood cells, are responsible for carrying oxygen from the lungs to various parts of the body for use in respiration. The HBB gene codes for one of the two types of polypeptide chains found in adult hemoglobin. Normal adult hemoglobin is a tetrameric protein consisting of two alpha chains and two beta chains. HBB codes for the beta chain, which is often referred to as beta globin. Mutant beta globin is responsible for the sickling of red blood cells seen in sickle cell anemia .

Steps for bio informatics:

Steps for bio informatics Open NCBI Write hemoglobin beta gene Press go/press fast Then biology workbench

Six frame for number of amino acid:

Six frame for number of amino acid

Name (not necessary): …………………………………………… Nucleotide sequence without name: (case insensitive, all letters except agctuswrymkhbdvn are disregarded) Nucleotide sequence: Output: nucleotides in one line; display the initial sequence; display complementary sequence in 5' --> 3' direction; Translation: Output: aminoacids in one line, code: Frame number: +1; +2; +3; all -1; -2; -3; Alignment: align amino acids sequences relative to nucleic acid. This tool is used to translate nucleic acid sequences. Use the “Rare Codon Search” tool to display any rare codons. For the manipulation of nucleic acid sequences (reverse, reverse/complement, double stranded) use the "Sequence Utilities" tool. Standard symbols for polymorph nucleotides: S g,C H A,C,T W A,T B C,g,T R A,g D A,g,T Y C,T V A,C,g M A,C N A,g,C,T K g,T :

Name (not necessary): …………………………………………… Nucleotide sequence without name: (case insensitive, all letters except agctuswrymkhbdvn are disregarded) Nucleotide sequence: Output: nucleotides in one line; display the initial sequence; display complementary sequence in 5' --> 3' direction; Translation: Output: aminoacids in one line, code: Frame number: +1; +2; +3; all -1; -2; -3; Alignment: align amino acids sequences relative to nucleic acid. This tool is used to translate nucleic acid sequences. Use the “Rare Codon Search” tool to display any rare codons. For the manipulation of nucleic acid sequences (reverse, reverse/complement, double stranded) use the "Sequence Utilities" tool. Standard symbols for polymorph nucleotides: S g,C H A,C,T W A,T B C,g,T R A,g D A,g,T Y C,T V A,C,g M A,C N A,g,C,T K g,T

GEN SCAN:

GEN SCAN

Sequence logo web site:

Sequence logo web site

www.pearl trees.com:

www.pearl trees.com

Metabolic pathway databases:

Metabolic pathway databases http://www.brenda-enzymes.org

Real time PCR PRIMER DATA BASES:

Real time PCR PRIMER DATA BASES rtprimerdb.org

Analysis for restriction sites web cutter:

Analysis for restriction sites web cutter

www.lifetechnologies.com:

www.lifetechnologies.com

New: the Real-Time PCR Handbook :

New: the Real-Time PCR Handbook

Thank u:

Thank u

References::

References: Genomics.energy.gov www.genscan.org www.ncbi.nlm.nih.gov

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