logging in or signing up distraction osteogenesis ppt drshahidkhan Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 513 Category: Entertainment License: All Rights Reserved Like it (1) Dislike it (0) Added: January 12, 2011 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Histomorphometric and Micro-CT analysis of the contralateral mandible during unilateral Mandibular Distraction Osteogenesis Daniel A Schwarz MD; Krikor Arman BS, Ayman Elmeligy MD, Ameen Jamali, Jordan Stewart and Steven R Buchman MD: Histomorphometric and Micro-CT analysis of the contralateral mandible during unilateral Mandibular Distraction Osteogenesis Daniel A Schwarz MD; Krikor Arman BS, Ayman Elmeligy MD, Ameen Jamali, Jordan Stewart and Steven R Buchman MDIntroduction: Introduction Craniofacial Surgeons DO mandible 15yrs Increased use craniofacial skeleton Currently study: Mechanism action; Metrics healing; New methods to enhance osteogenesisPurpose: Purpose Unilateral use in mandible Affect on contralateral mandible? No healing regenerate or fracture Micro-architecture of existing boneHypothesis: Hypothesis The forces involved with unilateral MDO will show a significant affect on the ultra-structure of contralateral hemi-mandibles, when compared to un-operated controls.Study Design - Methods: Study Design - Methods B ilateral f rame distractor Male SD rats (400g) Unilateral vertica l osteotomy Posterior to m olar sDistraction Osteogenesis: Ext ernal fixat or Gradual Distraction Biomechanical stress and strain Induced/transmitted affect Distraction OsteogenesisMicro-CT: Micro-CT Non-Destructive Quantitation of bone Microarchitecture at 45 µ resolution Bone Quality via Quantitative meansQuantitative Histomorphometry: Quantitative Histomorphometry De-Calcified 7 µm Paraffin Gomori Trichrome Bioquant ® ThresholdingSlide 9: ROI Template Calibrated between µ CT and Histology Reviewers BMD, BVF, vBMD ~ µ CT BV/TV ~ histologyStatistics: Statistics cC n = 26 µ CT; n = 6 Histology uC n = 18 µ CT; n = 6 Histology ANOVA w/ post hoc comparison Significance @ p < 0.05µCT Analysis: µCT Analysis BVF = % bone in ROIBMD and vBMD: BMD and vBMD BMD general for entire ROI vBMD specific for actual bone in ROI vBMD = BMD/ BVF ; More of Same cC uCSlide 14: Bioquant ® cC Total Volume uC Total VolumeSlide 15: Bioquant ® Bone Volume cC uCResults: Results Two separate modalities bone quantity: Significant ultrastructural changes contralateral mandible during unilateral MDO Increase both BMD and BVF supported by similar increase in BV/TV Increase quantity bone same densityCONCLUSIONS: CONCLUSIONS Increased bone in cC of MDO occurs due to a probable force induced upregulation in osteogenesis, or a potential decrease in bone resorption.CONCLUSIONS: CONCLUSIONS In line with the functional matrix theory, there are unexpected and unintended consequences which we must take into consideration when planning unilateral distraction osteogenesis.Acknowledgements: Acknowledgements Steve Goldstein, PhD Paul Makidon, DVD University of Michigan Orthopaedic Research Laboratories Carl’s Foundation You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
distraction osteogenesis ppt drshahidkhan Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 513 Category: Entertainment License: All Rights Reserved Like it (1) Dislike it (0) Added: January 12, 2011 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Histomorphometric and Micro-CT analysis of the contralateral mandible during unilateral Mandibular Distraction Osteogenesis Daniel A Schwarz MD; Krikor Arman BS, Ayman Elmeligy MD, Ameen Jamali, Jordan Stewart and Steven R Buchman MD: Histomorphometric and Micro-CT analysis of the contralateral mandible during unilateral Mandibular Distraction Osteogenesis Daniel A Schwarz MD; Krikor Arman BS, Ayman Elmeligy MD, Ameen Jamali, Jordan Stewart and Steven R Buchman MDIntroduction: Introduction Craniofacial Surgeons DO mandible 15yrs Increased use craniofacial skeleton Currently study: Mechanism action; Metrics healing; New methods to enhance osteogenesisPurpose: Purpose Unilateral use in mandible Affect on contralateral mandible? No healing regenerate or fracture Micro-architecture of existing boneHypothesis: Hypothesis The forces involved with unilateral MDO will show a significant affect on the ultra-structure of contralateral hemi-mandibles, when compared to un-operated controls.Study Design - Methods: Study Design - Methods B ilateral f rame distractor Male SD rats (400g) Unilateral vertica l osteotomy Posterior to m olar sDistraction Osteogenesis: Ext ernal fixat or Gradual Distraction Biomechanical stress and strain Induced/transmitted affect Distraction OsteogenesisMicro-CT: Micro-CT Non-Destructive Quantitation of bone Microarchitecture at 45 µ resolution Bone Quality via Quantitative meansQuantitative Histomorphometry: Quantitative Histomorphometry De-Calcified 7 µm Paraffin Gomori Trichrome Bioquant ® ThresholdingSlide 9: ROI Template Calibrated between µ CT and Histology Reviewers BMD, BVF, vBMD ~ µ CT BV/TV ~ histologyStatistics: Statistics cC n = 26 µ CT; n = 6 Histology uC n = 18 µ CT; n = 6 Histology ANOVA w/ post hoc comparison Significance @ p < 0.05µCT Analysis: µCT Analysis BVF = % bone in ROIBMD and vBMD: BMD and vBMD BMD general for entire ROI vBMD specific for actual bone in ROI vBMD = BMD/ BVF ; More of Same cC uCSlide 14: Bioquant ® cC Total Volume uC Total VolumeSlide 15: Bioquant ® Bone Volume cC uCResults: Results Two separate modalities bone quantity: Significant ultrastructural changes contralateral mandible during unilateral MDO Increase both BMD and BVF supported by similar increase in BV/TV Increase quantity bone same densityCONCLUSIONS: CONCLUSIONS Increased bone in cC of MDO occurs due to a probable force induced upregulation in osteogenesis, or a potential decrease in bone resorption.CONCLUSIONS: CONCLUSIONS In line with the functional matrix theory, there are unexpected and unintended consequences which we must take into consideration when planning unilateral distraction osteogenesis.Acknowledgements: Acknowledgements Steve Goldstein, PhD Paul Makidon, DVD University of Michigan Orthopaedic Research Laboratories Carl’s Foundation