Slide 1: PHARMACOGNOSTIC STUDY OF
CRUDE DRUGS CONTAINING Anthraquinone glycosides AJAY
A.U College of pharmaceutical sciences CONTENTS : : CONTENTS : Definition of Anthraquinone Glycosides.
Different Anthracene moieties .
Mechanism of action .
Conclusion. DEFINITION: : DEFINITION: Anthracene glycosides are the organic compounds from plants or animal sources which on enzymatic or acid hydrolysis gives one or more glycones (sugar moieties) along with aglycone.
Chemically these are the ACETALS or SUGAR ETHERS formed by the interaction of –OH group each of anthraquinone moiety and sugar ,with a loss of water molecule. Anthracene (Anthraquinone) Glycosides : Anthracene (Anthraquinone) Glycosides MECHANISM OF ACTION: : MECHANISM OF ACTION: Anthracenosides are PURGATIVE because of their irritating action on large intestine.
They stimulate the muscular structure i.e.., smooth muscles of large intestine through which peristalitic and large intestinal movements gets accelerated results in evacuation of intestinal contents.
Simultaneously the mucus secretion is stimulated and water absorption is hindered and soft stools will be formed.
Action starts after 8-12 hrs.
Activity is due to anthranols ,anthrones and dianthrones. CRUDE DRUGS CONTAINING ANTHRAQUINONE GLYCOSIDES : CRUDE DRUGS CONTAINING ANTHRAQUINONE GLYCOSIDES Senna
Cascara SENNA: : SENNA: INDIAN SENNA:
(senna folium ,senna-ki-patti, tinnevelly senna ,cassia senna.)
BIOLOGICAL SOURCE :It is obtained from dried leaflets of Cassia angustifolia Vahl belongs to family LEGUMINOSAE.
CULTIVATION AND COLLECTION OF INDIAN SENNA:
Soil-RED loamy/coarse gravelly soil.
1st sowing - feb-march.
2nd sowing - oct-november.
1st harvesting – when leafs turn to thick ,fully grown and greenish.
2nd harvesting- after 4-6 weeks of 1st harvesting.
3rd harvesting- after 2weeks of 2nd harvesting.
Heavy irrigation and water logging are avoided. ALEXANDRIAN SENNA
(folia sennae alexandrinae,)Bological source :It is obtained from dried leaflets of Cassia acutifolia Delile belong Family LEGUMINOSAE. MACROSCOPIC CHARACTERS: : MACROSCOPIC CHARACTERS: INDIAN SENNA ALEXANDRIAN SENNA
YELLOWISH-GREEN PALE-GREYISH GREEN.
7-8mm width and25 -60mm length 2-4cm long and 7-12mm wide.
Lanceolate,Asymetrical leaflets. Ovate- lanceolate, margin is curved
and Asymmetrical base. Microscopic of senna characters : Microscopic of senna characters Epidermis : unicelllular ,conical, thick walled warty trichomes.
stomata: paracytic or rubiaceous.
Palisade tisssue on both sides (iso bilateral leaf lets).
Calcium oxalate crystals are present.
Spongy mesophyll, patch of sclerenchyma, cluster sheath and presence of collenchyma are the characteristics of senna.
Indian senna Alexandrian senna
Vein-islet no 19.5 - 22.5 25 - 29.5
Stomatal index 17 – 20 11.4 – 13.3
Palisade ratio 7.5(u) 9.5(u)
5.1(L) 7(L) ADULTERANTS: : ADULTERANTS: Tinnevelly senna and alexandrian senna are adulterated with
DOG SENNA (papillose cells in lower epidermis)
PALTHE SENNA (No Anthraquinone glycosides)
MECCA and ARABIANSENNA (brown-green colour ,elongated)
SENNA PODS :
(senna legumes ,sennae fructus, senna fruit.)
Biological source : Dried nearly ripe fruits of Cassia acutifolia Delile and Cassia angustifolia Vahl belongs to leguminosae.
Collection and preparation:
Pods are collected and dried .
By sifting separate pods manually segregated into different qualities
Inferior quality used for preparation of galenicals. Macroscopic characters: : Macroscopic characters: Pale green-brown colour,4-5cm long
Pods are flat ,thin and oblong shape. Commercial products : ALOES: : ALOES: (ALOE,MUSSABBAR,KUMARI.)
Dried juice of leaves of Aloe barbadensis Miller (curacao aloes),
Aloe perryi Baker (socotrine aloes),
Aloe ferox Miller,
Aloe africana Miller and
Aloe spicata Baker (cape aloes)
belonging to family Liliaceae.
Cultivation and collection:
Cultivated by root suckers.
No water logging.
Juice is collected by a cut at leaf base.
Preparation of Aloes:
Curacao aloes: Place the leaves in kerosene tins and kept in tilted position to drain out all the juice and boil on large copper pans and pour in metal containers.
Cape Aloes :They are placed in ground in basin shaped depression which is lined by goat skin /canvas for 5-6 hrs.
Socotrine and Zanzibar Aloes: juice is collected in goat skin and solidify. MACROSCOPIC CHARACTERS: : MACROSCOPIC CHARACTERS: CURACAO CAPE SOCOTRINE ZANZIBAR
SRONG ODOUR NAUSEOUS NAuSEOUS -
BROWNISH-BLACK BROWNISH-GREEN BROWNISH-YELLLOW LIVER BROWN
OPAQUE MASS MASSES OPAQUE PASTY MASS BITTER TASTE BITTER TASTE BITTER TASTE -
UNEVEN FRACTURED GLASSY FRACTURED CONCHOIDAL EVEN
In leaf cuticle, epidermis, palisade, tissue, parenchymatous mesophyll. (mucilagenous )
. Mesophyll encloses vascular bundles which are covered with peri-cyclic layer.
Aloeitic cells contains Aloe gel.
Calcium oxalate crystals in parenchyma.
Aloepowder: In lactophenol reagent.
CURACAO CAPE SACOTRINE ZANZIBAR
Small needles transparent,brown large prism groups/ IRREGULAR
or slender prisms angular/irregular dispersed LUMPS
Crystalline amorphous ---- - --- -- Substituents & Adulterants : Substituents & Adulterants Socotrine, zanzibar, Natal aloes ,Mocha aloes with BLACK CATECHU.
Natal: Dry extract of aloe candelabra for cape aloes.
Mocha: Brownish black glossy fragments interior varieties.
Curacao aloes with BLACK CATECHU ,IRON,STONES.
COMMERCIAL PRODUCTS: RHUBARB: : RHUBARB: (Radix rheum , Revandchini , Rheum )
Biological source :Dried rhizomes of Rheum emodi Wallich, Rheum palmatum Linn ,Rheum webbianum Royle of family polygonaceae.
Collection and preparation:
Drug obtained from wild plants which are draught resistant .
Propagated by seeds ,rhizomes.
Harvested in spring season and the rhizomes are collected and cleaned & de-corticated ,dried and packed in wooden boxes. Macroscopy: : Macroscopy: In market we will find round or flat shape rhizomes.
Barrel /cylindrical/ conical shape rhizomes which are 8-10 cm long,4cm thick.
Flat shape rhizomes are 7-10cm long,3-6cm thick.
Sharp odour and bitter taste.
Drug with pink colour fracture are of high quality in nature.
In abnormal vascular bundles medullary rays are star shaped.
Star spots are in reddish orange.
Rosette calcium oxlate crystals starch grains are also seen.
In rheum officianalis star spots are irregular and in R.palmatum as a continous ring.
Rhaspartic rhubarb contains rhaponticin and lacks rhein, emodin or aloe emodin.
It has estrogenic activity and shows blue flouroscence UV –LIGHT. COMMERCIAL PRODUCTS: : COMMERCIAL PRODUCTS: CASCARA: : CASCARA: (Cascara segrada ,Sacred bark,Chittem bark,Cortex Rhamnipurshianae)
Biological source: Dried bark of Rhamnus purshiana De candolle of family Rhamnaceae.
Collection: it is collected in between april-august when it is readily separates from wood.
By longitudinal incission of 5-10cm.
Externally purplish brown an internally reddish brown.
Nauseous odour, bitter taste .
1-4mm thick in size.
Broken pieces small and flat occurs as quills/channels.
Internally longitudinal striations. Slide 22: R.Emodi , R.webianum R.officinale , R.palmatum MICROSCOPY: : MICROSCOPY: Contains cortex , sclerides , primary & secondary phloem
Cork has several layers of cells with yellowish brown substance.
Cortex with collenchyma.
Parenchyma is cellulosoic.
Calcium oxalate crystals are present.
Phloem fibres, sieve tubes are present.
Substituents and adulterants: Barks of R. californica , R.fallax and frangula bark are substituted for cascara.
Former bark has more uniform coat of lichens and wide medullary rays than original species. COMMECIAL
PRODUCTS : Slide 25: Sennosides A&B Sennosides C&D CHEMICAL CONSTITUENTS REFERANCES: : REFERANCES: PHARMACOGNOSY BY C.K.KOKATE,A.P.PUROHIT,S.B.GOKHALE.
PHARMACOGNOSY BY W.C. EVANS