Biofield Treated Triphenylmethane

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Thermal, Spectroscopical & Physical Characterization of Triphenylmethane was performed after the unique energy treatment in this study. Read here for the more details.

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Open Access Trivedi et al. J Chromatogr Sep Tech 2015 6:6 http://dx.doi.org/10.4172/2157-7064.1000292 Research Article Open Access Chromatography Separation T echniques Journal of Chromatography Separation Techniques ISSN: 2157-7064 Volume 6 • Issue 6 • 1000292 J Chromatogr Sep Tech ISSN: 2157-7064 JCGST an open access journal Physical Thermal and Spectroscopical Characterization of Biofield Treated Triphenylmethane: An Impact of Biofield Treatment Trivedi MK 1 Branton A 1 Trivedi D 1 Nayak G 1 Bairwa K 2 and Jana S 2 1 Trivedi Global Inc. 10624 S Eastern Avenue Suite A-969 Henderson NV 89052 USA 2 Trivedi Science Research Laboratory Pvt. Ltd. Hall-A Chinar Mega Mall Chinar Fortune City Hoshangabad Rd. Bhopal Madhya Pradesh India Corresponding author: Snehasis Jana Trivedi Science Research Laboratory Pvt. Ltd. Hall-A Chinar Mega Mall Chinar Fortune City Hoshangabad Rd. Bhopal-462 026 Madhya Pradesh India Tel: +91-755-6660006 Fax: +91-755- 6660006 Fax: +5231261163 E-mail: publicationtrivedisrl.com Received August 13 2015 Accepted September 08 2015 Published Setember 15 2015 Citation: Trivedi MK Branton A Trivedi D Nayak G Bairwa K et al. 2015 Physical Thermal and Spectroscopical Characterization of Biofeld Treated Triphenylmethane: An Impact of Biofeld Treatment. Method Validation and Estimation of the Uncertainty. J Chromatogr Sep Tech 6: 292. doi:10.4172/2157-7064.1000292 Copyright: © 2015 Trivedi MK et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use distribution and reproduction in any medium provided the original author and source are credited. Abstract Triphenylmethane is a synthetic dye used as antimicrobial agent and for the chemical visualization in thin layer chromatography of higher fatty acids fatty alcohols and aliphatic amines. The present study was an attempt to investigate the impact of biofeld treatment on physical thermal and spectroscopical charecteristics of triphenylmethane. The study was performed in two groups i.e. control and treatment. The treatment group subjected to Mr. Trivedi’s biofeld treatment. The control and treated groups of triphenylmethane samples were characterized using X-ray diffraction XRD surface area analyzer differential scanning calorimetry DSC thermogravimetric analysis TGA Fourier transform infrared FT-IR ultraviolet-visible UV-Vis spectroscopy and gas chromatography- mass spectrometry GC-MS. XRD study revealed decreases in average crystallite size 14.22 of treated triphenylmethane as compared to control sample. Surface area analysis showed a slight increase 0.42 in surface area of treated sample with respect to control. DSC thermogram of treated triphenylmethane showed the slight increase in melting point and latent heat of fusion with respect to control. TGA analysis of control triphenylmethane showed weight loss by 45.99 and treated sample showed weight loss by 64.40. The T max was also decreased by 7.17 in treated sample as compared to control. The FT-IR and UV spectroscopic result showed the similar pattern of spectra. The GC-MS analysis suggested a signifcant decrease in carbon isotopic abundance expressed in δ 13 C ‰ in treated sample about 380 to 524‰ as compared to control. Based on these results it is found that biofeld treatment has the impact on physical thermal and carbon isotopic abundance of treated triphenylmethane with respect to control. Keywords: Triphenylmethane Biofeld treatment X-ray difraction Diferential scanning calorimetry Termogravimetric analysis Gas chromatography-Mass Spectrometry GC-MS Abbreviations XRD: X-ray difraction DSC: Diferential scanning calorimetry TGA: Termogravimetric analysis DTA: Diferential thermal analysis FT-IR: Fourier transform infrared UV-Vis: Ultraviolet-visible GC- MS: Gas chromatography-mass spectrometry PM: Primary molecule Introduction Triphenylmethane is a hydrocarbon with molecular formula C 6 H 5 3 CH. It builds the basic skeleton of many synthetic dyes such as bromocresol green malachite green etc. and used as pH indicator and fuorescence agent 1. Boulos RA has reported its antimicrobial property 2. Triphenylmethane reported to inhibit 3-methyl- cholanthrene-induced neoplastic transformation of 10T1/2 cells in a dose-dependent manner and as a novel chemo preventive agent 3. Tis has been used as visualizing agentin thin-layer chromatography of higher chain fatty acids fatty alcohols and aliphatic amines 4. Recently triphenylmethane was reported as an alternative for mediated electronic transfer systems in glucose oxidase biofuel cells enzymatic biofuel cell. Te enzymatic biofuel cell is a type of fuel cell wherein the enzymes are used as a catalyst to oxidize its fuel instead of costly metals 5. Hugle et al. used triphenylmethane as a possible moderator material to reduces the speed of neutrons in nuclear chain reactions especially promising as cold neutrons moderator. It has a unique structure i.e. three aromatic phenyl groups surrounding one central carbon atom that is able to generate a stable radical ion 6. Diverse applications of triphenylmethane especially as forescent indicator mediator in biofuel and as a moderator had been suggested the importance of physicochemical property of triphenylmethane. It was previously reported that physical and thermal properties of molecule also afect its reactivity 78. Hence it is benefcial to fnd out an alternate approach that can improved the physicochemical properties of compounds like triphenylmethane which can enhance its usability. Recently biofeld treatment reported to alter the spectral properties of various pharmaceutical drugs like chloramphenicol and tetracycline and physicochemical properties of metals beef extract and meat infusion powder 9-11. Relation between mass and energy Emc 2 is well reported in literature 12. The mass solid matter is consist of energy and when this energy vibrates at a certain frequency it provides physical atomic and structural properties like size shape texture crystal structure and atomic weight to the matter 13. Similarly human body also consists with vibratory energy particles like protons neutrons and electrons 14. Due to vibrations in these particles an electrical impulse is generated that cumulatively forms electromagnetic field which is known as biofield 15. The human has the ability to harness the energy from the environment or Universe and transmit this energy into any object living or nonliving on the Globe. The objects receive the energy and respond into useful way this process is known as biofield treatment.

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Citation: Trivedi MK Branton A Trivedi D Nayak G Bairwa K et al. 2015 Physical Thermal and Spectroscopical Characterization of Biofeld Treated Triphenylmethane: An Impact of Biofeld Treatment. Method Validation and Estimation of the Uncertainty. J Chromatogr Sep Tech 6: 292. doi:10.4172/2157-7064.1000292 Page 2 of 8 Volume 6 • Issue 6 • 1000292 J Chromatogr Sep Tech ISSN: 2157-7064 JCGST an open access journal Termogravimetric analysis-diferential thermal analysis TGA-DTA Termal stability of control and treated triphenylmethane were analyzed using Mettler Toledo simultaneous TGA and diferential thermal analyzer DTA. Te samples were heated from room temperature to 400°C with a heating rate of 5°C/min under air atmosphere. Percent change in temperature at which maximum weight loss occurs in sample was calculated. Spectroscopic studies For determination of FT-IR and UV-Vis spectroscopic characters the treated sample was divided into two groups i.e. T1 and T2. Both treated groups were analyzed for their spectral characteristics using FT- IR and UV-Vis spectroscopy as compared to control triphenylmethane sample. While for GC-MS analysis the treated sample was divided into four groups i.e. T1 T2 T3 and T4 and all treated groups were analyzed along with control sample for isotopic abundance of carbon-13. FT-IR spectroscopic characterization FT-IR spectra of control and treated samples of triphenylmethane were recorded on Shimadzu’s Fourier transform infrared spectrometer Japan with frequency range of 4000-500 cm -1 . Te analysis was accomplished to evaluate the efect of biofeld treatment at atomic level like dipole moment force constant and bond strength in chemical structure 26. UV-Vis spectroscopic analysis UV spectra of control and treated samples of triphenylmethane were obtained from Shimadzu UV-2400 PC series spectrophotometer. A quartz cell with 1 cm and a slit width of 2.0 nm was used for analysis. Te study was carried out using wavelength in the range of 200-400 nm. Te UV spectra were analyzed to determine the efect of biofeld treatment on the energy gap of bonding and nonbonding transition of electrons 26. Gas chromatography-mass spectroscopy GC-MS analysis Te GC-MS analysis of control and treatment samples T1 T2 T3 and T4 of triphenylmethane were performed on Perkin Elmer/auto system XL with Turbo mass and electron ionization mode USA. Detection limit was set to 1 Pico gram and mass range was set to 10-650 amu. Te isotopic ratio 13 C/ 12 C was expressed by its deviation in treated triphenylmethane sample with respect to control. Te isotopic abundance of 13 C was computed on a delta scale per thousand. Te values of δ 13 C of treated samples were calculated using following equation 27. 13 Treated Control Control RR C 1000 R ‰ − δ × 1 Where R Treated and R Control are the ratio of intensity at m/z245/ m/z244 for δ 13 C in mass spectra of treated and control samples respectively. Results and Discussion XRD analysis XRD of control and treated triphenylmethane are presented in Figure 1. Te control triphenylmethane showed the XRD peaks at 2θ equals to 11.70° 11.91° 15.00° 18.24° 19.67° 22.52° 22.66° 23.99° 24.96° 26.19° and 28.71°. However the XRD difractogram of treated triphenylmethane showed the decrease in intensity of the peaks. XRD peaks in treated sample were appeared at 2θ equals to 11.92° 12.33° 14.87° 15.03° 18.25° 19.66° 20.76° 22.52° 23.99° 24.24° and 26.19°. Mr. Trivedi’s unique biofield energy is also called as The Trivedi Effect ® and reported to change various physicochemical thermal and structural properties of several metals 101617 and ceramics 18. In addition biofield treatment has been extensively studied in different fields such as agricultural science 1920 biotechnology research 21 and microbiology research 22-24. Conceiving the impact of biofeld treatment on various living and nonliving things the study aimed to evaluate the impact of biofeld treatment on spectral and physicochemical properties of triphenylmethane using diferent analytical techniques. Materials and Methods Study design Triphenylmethane was procured from Sisco Research Laboratories India. Te study was performed in two groups i.e. control and treatment. Te control sample was remained as untreated and treatment sample was handed over in sealed pack to Mr. Trivedi for biofeld treatment under laboratory conditions. Mr. Trivedi provided the biofeld treatment through his energy transmission process to the treatment group without touching the sample 9. Te control and treated samples of triphenylmethane were evaluated using various analytical techniques like X-ray difraction XRD surface area analyzer diferential scanning calorimetry DSC thermogravimetric analysis TGA Fourier transform infrared FT-IR ultraviolet-visible UV-Vis spectroscopy and gas chromatography-mass spectrometry GC-MS. X-ray difraction XRD study XRD analysis of triphenylmethane was performed on Phillips Holland PW 1710 X-ray difractometer with copper anode and nickel flter. Wavelength of X-ray used in XRD system was 1.54056 Å with scanning rate of 0.05° 2/s and a chart speed of 10 mm/2. Data obtained from XRD system were in the form of a chart of 2θ 10-100° vs. intensity. Te average crystallite size G of triphenylmethane was calculated using the following equation 25. G kλ/bCosθ Percent change in average crystallite size G t -G c /G c ×100 Where G c and G t are average crystallite size of control and treated powder samples respectively. Surface area analysis Surface area of control and treated triphenylmethane was measured using the Brunauer–Emmett–Teller BET surface area analyzer Smart SORB 90. Percent changes in surface area were calculated using following equation: Treated Control Control S S changeinsurfacearea 100 S − × Where S Control and S Treated are the surface area of control and treated samples respectively. Diferential scanning calorimetry DSC study Te control and treatment samples of triphenylmethane were analyzed using a Pyris-6 Perkin Elmer diferential scanning calorimeter DSC on a heating rate of 10°C/min under air atmosphere with air fow rate of 5 mL/min. An empty pan sealed with covered aluminum pan was used as a reference. Te melting temperature T m and latent heat of fusion ΔH were obtained from the DSC curve.

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Citation: Trivedi MK Branton A Trivedi D Nayak G Bairwa K et al. 2015 Physical Thermal and Spectroscopical Characterization of Biofeld Treated Triphenylmethane: An Impact of Biofeld Treatment. Method Validation and Estimation of the Uncertainty. J Chromatogr Sep Tech 6: 292. doi:10.4172/2157-7064.1000292 Page 3 of 8 Volume 6 • Issue 6 • 1000292 J Chromatogr Sep Tech ISSN: 2157-7064 JCGST an open access journal Te sharp and intense peak in XRD difractogram of control and treated samples suggested the crystalline nature of triphenylmethane in both samples. Te result showed that the XRD peaks were shifed afer biofeld treatment as 1191°→1192° 1500°→15.03° 18.24°→18.25° 19.67°→19.66° 24.96°→24.24° etc. moreover few peaks like 11.70° and 28.7° in control sample are disappeared or their intensity is decreased afer biofeld treatment. Te decrease in intensity of XRD peaks in biofeld treated triphenylmethane might be attributed to decrease in long-range order of the molecules. Te average crystallite size was calculated using Scherrer formula and the result are shown in Figure 2. Te average crystallite size of control triphenylmethane was found as 117.17 nm that was decreased to 100.51 nm in treated sample. Te result showed about 14.22 decrease in average crystallite size in treated sample as compared to control. It was reported that increase in internal micro strain leads to decrease the corresponding crystallite size of the material 27. Moreover Zhang et al. showed that presence of strain and increase atomic displacement from their ideal lattice positions causes reduction in crystallite size 28. Hence it is assumed that biofeld treatment may induce the internal strain in triphenylmethane. Tis might be the responsible for decrease in average crystallite size of the treated triphenylmethane as compared to control. Surface area analysis Te surface area of control and treated samples of triphenylmethane was determined using BET surface area analyzer and data are presented in Figure 3. Te control sample showed a surface area of 0.8243 m 2 /g however the treated sample of triphenylmethane showed a surface area of 0.8278 m 2 /g. Te result showed a slight increase in surface area 0.42 in the treated triphenylmethane sample as compared to control. Te increase in surface area might be correlated to particle size reduction due to high internal strain produced by biofeld treatment 29. Te increase in surface area may lead to increase in solubility 30 and reactivity of triphenylmethane as compared to control. DSC analysis DSC was used to analyze the melting temperature and latent heat of fusion ΔH of control and treated triphenylmethane. In solid materials substantial amount of interacting forces exist in atomic level that hold the atoms at their positions. ΔH is defned as the energy needed to overcome the interaction force to change the solid phase into liquid phase. Hence the energy provided during phase change i.e. ΔH is stored as potential energy of atoms. However melting point is related with kinetic energy of the atoms 31. DSC thermogram showed the melting temperature at 94.57°C in control and 95.11°C in treated sample Table 1 which revealed about 0.57 increase in melting temperature in treated sample of triphenylmethane with respect to control. Te melting temperature of triphenylmethane was well supported by literature data 32. Likewise the DSC thermogram exhibited the latent heat of fusion i.e. 85.05 J/g in control and 85.27 J/g in treated sample of triphenylmethane. Te result depicted about 0.26 change in latent heat of fusion of treated sample as compared to control. Termogravimetric analysis TGA/derivative thermogravimetry DTG analysis Te TGA and DTG analysis of control and treated samples of triphenylmethane are shown in Table 1. TGA thermogram of control triphenylmethane exhibited the onset temperature around 216.00°C that was end-set around 257.00°C with 45.99 weight loss. However the treated triphenylmethane started losing weight around 193.00°C and end-set around at 248.00°C with 64.40 weight loss Figure 4. Te result showed decrease in onset temperature of treated triphenylmethane by 10.65 as compared to control. Moreover DTG thermogram exhibited the maximum thermal decomposition Figure 1: XRD diffractogram of triphenylmethane. Figure 2: Average crystallite size of control and treated triphenylmethane. Figure 3: Surface area of control and treated triphenylmethane.

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Citation: Trivedi MK Branton A Trivedi D Nayak G Bairwa K et al. 2015 Physical Thermal and Spectroscopical Characterization of Biofeld Treated Triphenylmethane: An Impact of Biofeld Treatment. Method Validation and Estimation of the Uncertainty. J Chromatogr Sep Tech 6: 292. doi:10.4172/2157-7064.1000292 Page 4 of 8 Volume 6 • Issue 6 • 1000292 J Chromatogr Sep Tech ISSN: 2157-7064 JCGST an open access journal volatilization of treated triphenylmethane afer biofeld treatment. It might be due to alteration in internal energy that results into earlier vaporization of treated triphenylmethane sample as compared to control. temperature T max at 232.77°C in control sample and at 216.07°C in treated sample of triphenylmethane. Te result suggested about 7.17 decrease in T max of treated sample with respect to control. Te decrease in T max of treated sample might be correlated with increase in vaporization or Figure 4: TGA thermogram of control and treated triphenylmethane. S No Parameter Control Treated 1 Latent heat of fusion J/g 85.05 85.27 2 Melting point °C 94.57 95.11 3 Onset temperature °C 216.00 193.00 4 T max °C 232.77 216.07 Table 1: Thermal analysis of control and treated samples of triphenylmethane. T max : Temperature at maximum weight loss occurs.

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Citation: Trivedi MK Branton A Trivedi D Nayak G Bairwa K et al. 2015 Physical Thermal and Spectroscopical Characterization of Biofeld Treated Triphenylmethane: An Impact of Biofeld Treatment. Method Validation and Estimation of the Uncertainty. J Chromatogr Sep Tech 6: 292. doi:10.4172/2157-7064.1000292 Page 5 of 8 Volume 6 • Issue 6 • 1000292 J Chromatogr Sep Tech ISSN: 2157-7064 JCGST an open access journal FT-IR spectroscopic analysis Te recorded FT-IR spectra Figure 5 were analyzed based on theoretically predicted wavenumber and presented in Table 2. Te chemical structure of triphenylmethane consists with three phenyl groups attached with a methyl carbon. Terefore Te FT-IR spectra of triphenylmethane should contains the stretching and bending peaks mainly due to C-H and CC groups. Te C-H stretching was assigned to peak appeared at 3022 cm -1 in control and treated T1 and T2 samples. Te CC stretchings of phenyl ring carbon were assigned to vibrational peak observed at 1444-1597 cm -1 in all three samples i.e. control T1 and T2. Te C-H in-plane deformation peaks were attributed to vibrational peaks observed at 1031-1078 cm -1 in all three samples i.e. control T1 and T2. In addition the C-H out of plane deformation peaks were assigned to IR peak appeared at 605-758 cm -1 in control T1 and T2 samples. Te FT-IR spectrum of triphenylmethane was well supported with the literature data 33. Te FT-IR result suggested that the biofeld treatment did not induce any structural changes in the triphenylmethane sample with respect to control. UV-Vis spectroscopy UV spectrum of control triphenylmethane showed absorbance maxima λ max at 207.0 262.0 and 269.4 nm. Similar pattern of λ max was observed for both the treated samples T1 and T2 i.e. at 213.0 261.8 and 269.2 nm in T1 and 206.5 261.5 and 269.0 nm in T2. Te result exhibited a slight bathochromic shif of peak at 213.0 nm in T1 as compared to control 207.0 nm. Apart from this other peaks were observed at the similar wavelength in all three samples i.e. control T1 and T2. Overall the UV spectral analysis suggests that biofeld treatment did not cause any signifcant alterations in the λ max of treated molecules as compared to control. Te fndings of UV analysis were also supported by the FT-IR results. GC-MS analysis Te GC-MS spectra of control and treated T1 T2 T3 and T4 samples of triphenylmethane are shown in Figures 6a and 6b and the peak intensity of molecular ion and most probable isotopes are illustrated in Table 3. Te GC-MS spectra of control and treated samples showed the primary molecule PM triphenylmethane peak at m/z 244 which suggested the molecular weight of triphenylmethane. In addition the peak at m/z 245 was assigned to isotopic abundance peaks due to PM +1 13 C/ 12 C. It is assumed that isotopic abundance ratio of PM +1 was mainly due to 13 C and 2 H isotopes in triphenylmethane. Based on this it is speculated that the peak at m/z 244 is may be due to Figure 5: FT-IR spectra of control and treated T1 and T2 triphenylmethane. Figure 6a: GC-MS spectra of triphenylmethane control. Wave number cm -1 Frequency assigned Control T1 T2 3022 3022 3022 C-H stretching 1444-1597 1444-1597 1444-1597 CC stretching 1031-1078 1031-1078 1031-1078 C-H in-plane deformation 605-758 605-758 605-758 C-H out of plane deformation Table 2: FT-IR Spectral analysis of triphenylmethane.

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Citation: Trivedi MK Branton A Trivedi D Nayak G Bairwa K et al. 2015 Physical Thermal and Spectroscopical Characterization of Biofeld Treated Triphenylmethane: An Impact of Biofeld Treatment. Method Validation and Estimation of the Uncertainty. J Chromatogr Sep Tech 6: 292. doi:10.4172/2157-7064.1000292 Page 6 of 8 Volume 6 • Issue 6 • 1000292 J Chromatogr Sep Tech ISSN: 2157-7064 JCGST an open access journal Figure 6b: GC-MS spectra of triphenylmethane T1 T2 T3 and T4.

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Citation: Trivedi MK Branton A Trivedi D Nayak G Bairwa K et al. 2015 Physical Thermal and Spectroscopical Characterization of Biofeld Treated Triphenylmethane: An Impact of Biofeld Treatment. Method Validation and Estimation of the Uncertainty. J Chromatogr Sep Tech 6: 292. doi:10.4172/2157-7064.1000292 Page 7 of 8 Volume 6 • Issue 6 • 1000292 J Chromatogr Sep Tech ISSN: 2157-7064 JCGST an open access journal Parameter Control Treated T1 T2 T3 T4 Peak intensity at m/z244 PM 93.45 57.42 89.16 72.87 71.08 Peak intensity at m/z245 PM +1 38.17 11.47 22.58 14.51 13.81 Ration of peak intensity 100 × PM +1 / PM 40.845 19.976 25.325 19.912 19.429 Carbon isotopic abundance δ 13 C ‰ with respect to control -510.9 -380.0 -512.5 -524.3 Table 3: GC-MS isotopic abundance analysis of triphenylmethane. PM: Primary molecule triphenylmethane. 12 C 19 1 H 16 and m/z 245 is due to 13 C 1 12 C 18 1 H 16 and 12 C 19 2 H 1 1 H 15 . Te GC- MS analysis result Table 3 showed that carbon isotopic abundance expressed in δ 13 C ‰ was changed as -510.9 -380.0 -512.5 and -524.3‰ in T1 T2 T3 and T4 respectively with respect to control. Te result depicted that in the entire treated samples T1 T2 T3 and T4 the 13 C and 2 H were transformed into 12 C and 1 H by releasing a neutron from their higher isotopes. Tis conversion of 13 C→ 12 C and 2 H→ 1 H might be possible if a nuclear level reaction occurred due to infuence of biofeld treatment. Based on this it is postulated that biofeld treatment possibly induced the nuclear level reactions in triphenylmethane which may lead to convert the 13 C into 12 C and 2 H into 1 H in treated sample as compared to control. Conclusions XRD difractogram of biofeld treated triphenylmethane showed the alteration in intensity of XRD peaks and average crystallite size 14.22 as compared to control. Te surface area analysis showed the slight increase in surface area of treated triphenylmethane with respect to control. Te thermal analysis DSC TGA/DTG showed a slight change in melting temperature and latent heat of fusion in treated triphenylmethane as compared to control. Te T max was also decreased by 7.17 in treated sample as compared to control. Te spectroscopic analysis FT-IR and UV-Vis showed that biofeld treatment did not afect the dipole moment bond force constant and the absorbance maxima λ max of treated sample as compared to control. GC-MS analysis showed the alteration in carbon isotopic abundance δ 13 C as -510.9 -380.0 -512.5 and -524.3‰ in T1 T2 T3 and T4 respectively as compared to control. Overall the physical thermal and spectroscopical study suggests the impact of biofeld treatment on physicochemical properties of treated triphenylmethane with respect to control. Based on this it is assumed that treated triphenylmethane could be more useful as compared to control. Acknowledgements Te authors would like to acknowledge the whole team of MGV pharmacy college Nashik for providing the instrumental facility. We would also like to thank Trivedi Science ™ Trivedi Master Wellness ™ and Trivedi Testimonials for their consistent support during the work. References 1. Kolling OW Smith ML 1959 Selected triphenylmethane dyes as acid-base indicators in glacial acetic acid. Anal Chem 31: 1876-1879. 2. Boulos RA 2013 Antimicrobial dyes and mechanosensitive channels. Antonie Van Leeuwenhoek 104: 155-167. 3. Cooney RV Pung A Harwood PJ Boynton AL Zhang LX et al. 1992 Inhibition of cellular transformation by triphenylmethane: a novel chemopreventive agent. Carcinogenesis 13: 1107-1112. 4. Kwapniewski Z Cichon R 1979 The application of triphenylmethane dyes to visualization of selected aliphatic compounds in thin-layer chromatography. Microchem J 24: 298-299. 5. La Rotta H CE Ciniciato GP González ER 2011 Triphenylmethane dyes an alternative for mediated electronic transfer systems in glucose oxidase biofuel cells. Enzyme Microb Technol 48: 487-497. 6. Hugle T Mocko M Hartl MA Daemen LL Muhrer G 2014 Triphenylmethane a possible moderator material. Nucl Instrum Methods Phys Res A 738: 1-5. 7. Carballo LM Wolf EE 1978 Crystallite size effects during the catalytic oxidation of propylene on Pt /γ-Al 2 O 3 . J Catal 53: 366-373. 8. Chaudhary AL Sheppard DA Paskevicius M Pistidda C Dornheim M et al. 2015 Reaction kinetic behaviour with relation to crystallite/grain size dependency in the Mg-Si-H system. Acta Mater 95: 244-253. 9. Trivedi MK Patil S Shettigar H Bairwa K Jana S 2015 Spectroscopic characterization of chloramphenicol and tetracycline: An impact of biofeld. Pharm Anal Acta 6: 1-5. 10. Trivedi MK Patil S Tallapragada RM 2013 Effect of bio feld treatment on the physical and thermal characteristics of silicon tin and lead powders. 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Trivedi MK Patil S Tallapragada RM 2013 Effect of biofeld treatment on the physical and thermal characteristics of vanadium pentoxide powders. J Material Sci Eng S11: 001. 19. Sances F Flora E Patil S Spence A Shinde V 2013 Impact of biofeld treatment on ginseng and organic blueberry yield. Agrivita J Agric Sci 35. 20. Lenssen AW 2013 Biofeld and fungicide seed treatment infuences on soybean productivity seed quality and weed community. Agricultural Journal 8: 138-143. 21. Patil SA Nayak GB Barve SS Tembe RP Khan RR 2012 Impact of biofeld treatment on growth and anatomical characteristics of Pogostemon cablin Benth.. Biotechnology 11: 154-162. 22. Trivedi MK Patil S 2008 Impact of an external energy on Staphylococcus epidermis ATCC-13518 in relation to antibiotic susceptibility and biochemical reactions-an experimental study. J Accord Integr Med 4: 230-235. 23. Trivedi MK Patil S 2008 Impact of an external energy on Yersinia enterocolitica ATCC-23715 in relation to antibiotic susceptibility and biochemical reactions: an experimental study. Internet J Alternat Med 6: 1-6. 24. Trivedi MK Patil S Shettigar H Gangwar M Jana S 2015 Antimicrobial sensitivity pattern of Pseudomonas fuorescens after biofeld treatment. J Infect Dis Ther 3: 222. 25. Patterson AL 1939 The Scherrer formula for X-Ray particle size determination. Phys Rev 56: 978-982. 26. Pavia DL Lampman GM Kriz GS 2001 Introduction to spectroscopy. 3rd edn Thomson Learning Singapore. 27. Paiva-Santos CO Gouveia H Las WC Varela JA 1999 Gauss-Lorentz size- strain broadening and cell parameters analysis of Mn doped SnO 2 prepared by organic route. Mat Structure 6: 111-115. 28. Zhang K Alexandrov IV Kilmametov AR Valiev RZ Lu K 1997 The crystallite- size dependence of structural parameters in pure ultrafne-grained copper. J Phys D Appl Phys 30: 3008-3015. 29. Trivedi MK Nayak G Tallapragada RM Patil S Latiyal O et al. 2015 Effect of biofeld treatment on structural and morphological properties of silicon carbide. J Powder Metall Min 4: 1-4.

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Citation: Trivedi MK Branton A Trivedi D Nayak G Bairwa K et al. 2015 Physical Thermal and Spectroscopical Characterization of Biofeld Treated Triphenylmethane: An Impact of Biofeld Treatment. Method Validation and Estimation of the Uncertainty. J Chromatogr Sep Tech 6: 292. doi:10.4172/2157-7064.1000292 Page 8 of 8 Volume 6 • Issue 6 • 1000292 J Chromatogr Sep Tech ISSN: 2157-7064 JCGST an open access journal 30. Hansen CM 2007 Hansen Solubility Parameters: A Users Handbook. 2nd edn CRC press. 31. Jamin E Martin F Martin GG 2004 Determination of the 13C/12C ratio of ethanol derived from fruit juices and maple syrup by isotope ratio mass spectrometry: collaborative study. J AOAC Int 87: 621-631. Citation: Trivedi MK Branton A Trivedi D Nayak G Bairwa K et al. 2015 Physical Thermal and Spectroscopical Characterization of Biofeld Treated Triphenylmethane: An Impact of Biofeld Treatment. Method Validation and Estimation of the Uncertainty. J Chromatogr Sep Tech 6: 292. doi:10.4172/2157- 7064.1000292 32. Cornish hh Zamora E Bahor RE 1964 Metabolism of Triphenylmethane and Triphenylcarbinol. Arch Biochem Biophys 107: 319-324. 33. Cheriaa J Khaireddine M Rouabhia M Bakhrouf A 2012 Removal of triphenylmethane dyes by bacterial consortium. ScientifcWorld Journal 2012: 512454. OMICS International: Publication Benefits Features Unique features: • Increased global visibility of ar ticles thr ough w or ld wide distribution and inde xing • Sho w casing recent researc h output in a timely and updated manner • Special issues on the current trends of scientifc researc h Special features: • 700 Open Access J our nals • 50000 Editorial team • R apid revie w pr ocess • Quality and quic k editorial revie w and publication pr ocessing • Inde xing at PubMed par tial Scopus EBSCO Inde x Coper nicus Google Sc holar etc. • Sharing Option: Social Netw or king Enabled • Authors R evie w ers and Editors re w arded with online Scientifc Credits • Better discount f or y our subsequent ar ticles Submit your manuscript at: http://www.omicsgroup.org/journals/submission

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