3-radial glial cells

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This hybridoma produces mAbs against Radial glia and neural progenitor cells. https://www.creative-diagnostics.com/Anti-Radial-glia-and-neural-progenitor-cells-hybridoma-137099-197.htm

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Creative Diagnostics. All rights reserved 45-16 Ramsey Road Shirley NY 11967 USA Tel: 631-624-4882 ·Fax:631-614-7828 E-mail: infocreative-diagnostics.com www.creative-diagnostics.com Mouse Anti-Radial glia and neural progenitor cells hybridoma SD2 Anti-Radial glia and neural progenitor cells hybridoma Lot. No. See product label CELL LINE INFORMATION Cat.No. CSC-H1480 Common Name Radial glia and neural progenitor cells Clone SD2 Cell Line Description This cell line produces a monoclonal antibody RC1 IgG class. Immunochistochemically RC1 antibody reacts with radial glia and neural progenitor cells in CNS. Introduction Radial glial cells are a pivotal cell type in the developing central nervous system CNS involved in key developmental processes from patterning and neuronal migration to their recently discovered role as precursors during neurogenesis. They arise early in development from neuroepithelial cells. Radial phenotype is typically transient but some cells such as Bergmann glia in the cerebellum and Muller glia in the retina retain radial glia-like morphology postnatally. According to recent research during the late stages of cortical development radial glial cells divide asymmetrically in the ventricular zone to generate radial glial cells postmitotic neurons and intermediate progenitor cells. Intermediate progenitor cells then divide symmetrically in the subventricular Neural progenitor cells are more promising candidates for replacing damaged and degenerated neurons because they are self- renewing which allows for the in vitro production of many cells with minimal donor material. In order to confirm that the new neurons formed from neural progenitor cells are a part of a functional network the presence of synapse formation is required. A study by Ma Fitzgerald et al. is the first demonstration of murine neural stem and progenitor cell-derived functional synapse and neuronal network formation on a 3D collagen matrix. The neural progenitor cells expanded and spontaneously differentiated into excitable neurons and formed synapses furthermore they retained the ability to differenitate into the three neural tissue lineages. It was also demonstrated that not only active synaptic vesicle recycling occurred but also that excitatory and inhibitory connections capable of generating action potentials spontaneously were formed. Thus neural progenitor cells are a viable and relatively unlimited source for creating functional neurons. Immunogen Radial glia and neural progenitor cells Immunological Donor Mouse spleen Fusion Species Mouse x Mouse Hybridoma Morphology round Propagation Complete growth medium: RPMI1640 medium with 15 fetal bovine serum Atmosphere: air 95 carbon dioxide CO2 5 Temperature: 37.0 °C Culture Medium RPMI1640 medium with 15 fetal bovine serum Mycoplasma Mycoplasma Status: Negative MycoAlert Kit ANTIBODY INFORMATION Target Radial glia and neural progenitor cells Application N/A SAFETY AND PACKAGING Storage liquid nitrogen

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Creative Diagnostics. All rights reserved 45-16 Ramsey Road Shirley NY 11967 USA Tel: 631-624-4882 ·Fax:631-614-7828 E-mail: infocreative-diagnostics.com www.creative-diagnostics.com Safety Considerations The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating drinking or smoking while handling the hybridoma. 3. Wash hands after handling the hybridoma and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70 ethanol before and after working with hybridoma. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. Ship Dry Ice

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