Single Stranded (SSCP)

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ATCTATC AACGAGC ATCTATC ATCTATC + - ATCTATC SSCPs Single Stranded Conformation Polymorphism Asymmetric PCR Less Concentration More Concentration Target Gene ds DNA ds DNA with different sequence Will Yield ss DNA. DNAs of same length with different nucleotide sequence would also show polymorphism due to formation of secondary and tertiary structures. This is otherwise not possible using dsDNA It can be used with short DNA fragments Only . Can detect variation between the fragments of equal molecular weights. 0.8% 0.8% 0.8% 0.8% 0.8% 0.6%