Linking biotechnology with plant breeding

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PLASMID METHOD…………….Biological PARTICLE BOMBARDMENT DIRECT DNA UPTAKE MICROINJECTION Host Specificity Somaclonal Variation Protoplast Regeneration Cost Monocots and Dicots Non-biological approaches TRANSGENICS Tissue Culture + Genetic Engineering PLANT BIOTECHNOLOGY Bacteria Isolate Bacterial Gene Clone BYPASS OF SEXUAL PROCESS Transformations Manipulation of Foreign Gene Used in Back Cross Program Cultivated Variety (-) TRANSGENIC (+) Plant x BC6-BC8 CONVENTIONAL MARKER-ASSISTED SELECTION Markers RFLP RAPD AFLP SSR SNP etc. BIOTECHNOLOGY IN CROP IMPROVEMENT Protoplasts or cell cultures METHODS OF FOREIGN GENE TRANSFER Features: Character Quantitative/Qualitative Character Types of molecular markers When they are required? Advantages and Disadvantages Would Mol.Markers substitute Plant Breeding Methods? Usefulness of different markers How to start with Mol. Mar. in new lab. What should be your first objective? Can you start molecular work without enough lab facilities? Are computers and internet useful in molecular analysis? © A.K. Chhabra Sample Preparation LICOR AFLP