DNA Markers-SSR

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Slide 1: 

…….AGCTAATCTAGAGAGAGAGAGAGCTAGCTGATTCAACTAC……. SSR …….TCGATTAGATCTCTCTCTCTCTCGATCGACTATGTTGATG……. (GA)5 (CT)5 GATTAGATCT GCTAGCT Sequencing required Detects FIXED LOCUS Co-dominant marker Useful for mapping studies Highly reproducible Not useful for comparative mapping Requires more facilities for Primer Synthesizing Highly polymorphic than RFLP, RAPD and AFLP More uniformly distributed in the genome Examples: 37 alleles in barley (Saghai-Maroof et al 1997) 26 alleles in soybeans (Rongwen et al. 1995)

Slide 2: 

SSRs are multiallelic (GA)20 (GA)18 (GA)15 (GA)12 (GA)4 (GA)2 P1 P2 P3 P4 P5 P6 P7 P8 P9 P10 P11 P12

Multiplexing of SSRs : 

Multiplexing of SSRs Poly Poly Poly Poly Poly Poly Poly Poly Poly Mono Mono Mono Polymorphic: 9 Monomorphic: 3 Primer pairs Did not work

Slide 4: 

Multiplexing of SSRs Poly Poly Poly Poly Poly Poly Poly Poly Poly Mono Mono Mono Polymorphic: 9 Monomorphic: 3 Primer pairs M M 1 2 3 4 5 6 7 8 9 10 11 12 13