logging in or signing up Barnase Barstar Gene of male sterility chhabra61 Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 2067 Category: Education License: All Rights Reserved Like it (7) Dislike it (0) Added: August 11, 2010 This Presentation is Public Favorites: 1 Presentation Description No description available. Comments Posting comment... By: NARU101 (7 month(s) ago) Respected sir, i have seen your ppt and u have done a great work here, sir i am a student of Plant Breeding and Genetics and i am going to give a presentation on barnase barstar system, so i need your presentation especialy barnase-barstar part. it is a great presentation i will be very thankfull to you if your goodself can send this preseull to you plzz send it as soon as possiple hope for your positive replyntation my id Janardand03@gmail.com Saving..... Post Reply Close Saving..... Edit Comment Close By: geemalik (19 month(s) ago) respected sir i am very need of your presentation especially barnase-barstar part ,,it is a great presentation i will be very thankfull to you if your goodself can send this preseull to you plzz send it as soon as possiple hope for your positive replyntation my id geemalik06@gmail.com i will be highly thank f Saving..... Post Reply Close Saving..... Edit Comment Close By: patilna (19 month(s) ago) Male sterility is a very nice and informatic presentation Saving..... Post Reply Close Saving..... Edit Comment Close By: tanerfatih (19 month(s) ago) very well study. thanks Saving..... Post Reply Close By: vikramjambhale (1 month(s) ago) Kindly permit to download the presenation Saving..... Edit Comment Close Premium member Presentation Transcript Slide 1: Barnase gene…produces RNAse Barnase gene…produces RNAse TA29 Barnase gene Tissue specific (tapetum layer) promoter Herbicide Resistance (Phosphinothricin) (Gene construct) Endothecium Middle layer Tapetum Bacillus amyloliquefaciens Tapetum degenerates PMCs/pollen become sterile BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE © A.K. Chhabra 2007 Slide 2: Tapetum persistant PMCs/pollen are fertile Barnase gene…produces RNAse Barstar gene TA29 Barnase gene Tissue specific (tapetum layer) promoter Herbicide Resistance (Phosphinothricin) (Gene construct) Inhibits RNAse Endothecium Middle layer Tapetum Bacillus amyloliquefaciens BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE © A.K. Chhabra 2007 Slide 3: Barnase gene…produces RNAse Barstar gene (fertility restorer) TA29 Barnase gene Tissue specific (tapetum layer) promoter Herbicide Resistance (Phosphinothricin) (Gene construct) Inhibits RNAse Endothecium Middle layer Tapetum Bacillus amyloliquefaciens Tapetum degenerates PMCs/pollen become sterile Tapetum persistant PMCs/pollen are fertile BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE Dominant male sterility Barstar-Barnase interaction (binding) © A.K. Chhabra 2007 Slide 4: Barnase gene Tissue specific (tapetum layer) promoter Herbicide Resistance (Phosphinothricin) (Gene construct) BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY Male sterile Herbicide resistant Male Fertile Herbicide sensitive Herbicide spray PRINCIPLE © A.K. Chhabra 2007 Slide 5: BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY Male Fertile KILLED PRINCIPLE © A.K. Chhabra 2007 Slide 6: BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY Barnase-Bar/- - /- x Sterile Herbicide Resistant Fertile Herbicide Sensitive 50% Barnase-Bar/- (male sterile, rest.) 50% -/- (male fertile, susceptible) Phosphinothricin Spray Killed , Killed , Killed , Killed X Barstar/Barstar Fertile Restorer 50% Barnase-Bar/Barstar (male fertile) 50% -/Barstar (male fertile) 100% male fertile F1 Hybrid STEP 1 Maintenance of male sterility STEP 2 Production of Hybrid © A.K. Chhabra 2007 CHEMICALLY INDUCED MALE STERILITY : CHEMICALLY INDUCED MALE STERILITY MALE STERILE MALE STERILE MALE STERILE MALE FERTILE CHEMICALLY INDUCED MALE STERILITY : CHEMICALLY INDUCED MALE STERILITY Induced by spray of certain chemicals GA (gibberellic acid) NAA (napthalene acetic acid) MH (Maleic Hydrazide) Etheral MALE GAMETOCIDES CHAs Chemical Hybridizing Agents SUCCESSFUL EXAMPLES CHEMICAL CROP Etherel Rice, Sugarbeet, Wheat FW 450 Cotton, Groundnut, Sugarbeet, Tomato GA (Gibberellic Acid) Lettuce, Maize, Onion, Rice, Sunflower MH (Maliec Hydrazide) Cucurbits, Onion, Tomato, Wheat NAA (Napthelene Acetic Acid) Cucurbits Sodium Methyl Arsenate Rice Zinc Methyl Arsenate Rice CHEMICALLY INDUCED MALE STERILITY : CHEMICALLY INDUCED MALE STERILITY Characteristics of an Ideal CHA Female fertility should be unaffected Be non-mutagenic Consistent complete male sterility Easy and economical to apply Minimum side effects Safe to user and envt. NO SUCH CHA AVAILABLE Advantages of using CHAs Effective for the generation applied No transfer of CMS required Can be applied to the variety required only B and R are not required Saves time, labour and money to develop A, B, and R lines CHEMICALLY INDUCED MALE STERILITY : CHEMICALLY INDUCED MALE STERILITY Chief limitations Pollen abortion------incomplete and variable Specific developmental stages are sensitive to CHAs Effect of CHAs are short lived Female fertility may be reduced Often undesirable side effects are produced High cost of CHAs The need for repeated applications increases the cost of hybrid seed Transfer of cytoplasm from male sterile to a new strain : S Transfer of cytoplasm from male sterile to a new strain 50 % rr F rr X rr Slide 12: 75 % rr F X rr Transfer of cytoplasm from male sterile to a new strain S Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 87.5 % rr F X rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 93.75 % rr F X rr rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 96.87 % rr F X rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 98.94 % rr F X rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 99.47 % rr F X rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 99.73 % rr F X rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 99.87 % rr F X rr You do not have the permission to view this presentation. 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Barnase Barstar Gene of male sterility chhabra61 Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 2067 Category: Education License: All Rights Reserved Like it (7) Dislike it (0) Added: August 11, 2010 This Presentation is Public Favorites: 1 Presentation Description No description available. Comments Posting comment... By: NARU101 (7 month(s) ago) Respected sir, i have seen your ppt and u have done a great work here, sir i am a student of Plant Breeding and Genetics and i am going to give a presentation on barnase barstar system, so i need your presentation especialy barnase-barstar part. it is a great presentation i will be very thankfull to you if your goodself can send this preseull to you plzz send it as soon as possiple hope for your positive replyntation my id Janardand03@gmail.com Saving..... Post Reply Close Saving..... Edit Comment Close By: geemalik (19 month(s) ago) respected sir i am very need of your presentation especially barnase-barstar part ,,it is a great presentation i will be very thankfull to you if your goodself can send this preseull to you plzz send it as soon as possiple hope for your positive replyntation my id geemalik06@gmail.com i will be highly thank f Saving..... Post Reply Close Saving..... Edit Comment Close By: patilna (19 month(s) ago) Male sterility is a very nice and informatic presentation Saving..... Post Reply Close Saving..... Edit Comment Close By: tanerfatih (19 month(s) ago) very well study. thanks Saving..... Post Reply Close By: vikramjambhale (1 month(s) ago) Kindly permit to download the presenation Saving..... Edit Comment Close Premium member Presentation Transcript Slide 1: Barnase gene…produces RNAse Barnase gene…produces RNAse TA29 Barnase gene Tissue specific (tapetum layer) promoter Herbicide Resistance (Phosphinothricin) (Gene construct) Endothecium Middle layer Tapetum Bacillus amyloliquefaciens Tapetum degenerates PMCs/pollen become sterile BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE © A.K. Chhabra 2007 Slide 2: Tapetum persistant PMCs/pollen are fertile Barnase gene…produces RNAse Barstar gene TA29 Barnase gene Tissue specific (tapetum layer) promoter Herbicide Resistance (Phosphinothricin) (Gene construct) Inhibits RNAse Endothecium Middle layer Tapetum Bacillus amyloliquefaciens BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE © A.K. Chhabra 2007 Slide 3: Barnase gene…produces RNAse Barstar gene (fertility restorer) TA29 Barnase gene Tissue specific (tapetum layer) promoter Herbicide Resistance (Phosphinothricin) (Gene construct) Inhibits RNAse Endothecium Middle layer Tapetum Bacillus amyloliquefaciens Tapetum degenerates PMCs/pollen become sterile Tapetum persistant PMCs/pollen are fertile BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE Dominant male sterility Barstar-Barnase interaction (binding) © A.K. Chhabra 2007 Slide 4: Barnase gene Tissue specific (tapetum layer) promoter Herbicide Resistance (Phosphinothricin) (Gene construct) BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY Male sterile Herbicide resistant Male Fertile Herbicide sensitive Herbicide spray PRINCIPLE © A.K. Chhabra 2007 Slide 5: BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY Male Fertile KILLED PRINCIPLE © A.K. Chhabra 2007 Slide 6: BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY Barnase-Bar/- - /- x Sterile Herbicide Resistant Fertile Herbicide Sensitive 50% Barnase-Bar/- (male sterile, rest.) 50% -/- (male fertile, susceptible) Phosphinothricin Spray Killed , Killed , Killed , Killed X Barstar/Barstar Fertile Restorer 50% Barnase-Bar/Barstar (male fertile) 50% -/Barstar (male fertile) 100% male fertile F1 Hybrid STEP 1 Maintenance of male sterility STEP 2 Production of Hybrid © A.K. Chhabra 2007 CHEMICALLY INDUCED MALE STERILITY : CHEMICALLY INDUCED MALE STERILITY MALE STERILE MALE STERILE MALE STERILE MALE FERTILE CHEMICALLY INDUCED MALE STERILITY : CHEMICALLY INDUCED MALE STERILITY Induced by spray of certain chemicals GA (gibberellic acid) NAA (napthalene acetic acid) MH (Maleic Hydrazide) Etheral MALE GAMETOCIDES CHAs Chemical Hybridizing Agents SUCCESSFUL EXAMPLES CHEMICAL CROP Etherel Rice, Sugarbeet, Wheat FW 450 Cotton, Groundnut, Sugarbeet, Tomato GA (Gibberellic Acid) Lettuce, Maize, Onion, Rice, Sunflower MH (Maliec Hydrazide) Cucurbits, Onion, Tomato, Wheat NAA (Napthelene Acetic Acid) Cucurbits Sodium Methyl Arsenate Rice Zinc Methyl Arsenate Rice CHEMICALLY INDUCED MALE STERILITY : CHEMICALLY INDUCED MALE STERILITY Characteristics of an Ideal CHA Female fertility should be unaffected Be non-mutagenic Consistent complete male sterility Easy and economical to apply Minimum side effects Safe to user and envt. NO SUCH CHA AVAILABLE Advantages of using CHAs Effective for the generation applied No transfer of CMS required Can be applied to the variety required only B and R are not required Saves time, labour and money to develop A, B, and R lines CHEMICALLY INDUCED MALE STERILITY : CHEMICALLY INDUCED MALE STERILITY Chief limitations Pollen abortion------incomplete and variable Specific developmental stages are sensitive to CHAs Effect of CHAs are short lived Female fertility may be reduced Often undesirable side effects are produced High cost of CHAs The need for repeated applications increases the cost of hybrid seed Transfer of cytoplasm from male sterile to a new strain : S Transfer of cytoplasm from male sterile to a new strain 50 % rr F rr X rr Slide 12: 75 % rr F X rr Transfer of cytoplasm from male sterile to a new strain S Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 87.5 % rr F X rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 93.75 % rr F X rr rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 96.87 % rr F X rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 98.94 % rr F X rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 99.47 % rr F X rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 99.73 % rr F X rr Transfer of cytoplasm from male sterile to a new strain : Transfer of cytoplasm from male sterile to a new strain 99.87 % rr F X rr