Slide 2:
How
Substitution leads to RFLP………………… AGCTTATTCGGATTCAAGGATCCTTCGGATTCAACTA MUTATED
G to T AGCTTATTCGTATTCAAGGATCCTTCGG ATTCAACTA RESTRICTION
FRAGMENTS AGCTTATTCGGTTTCAAGGATCCTTCGGATTCAACTA Results into
Restriction Fragment Length
POLYMOPRPHISM deletion
insertion and/or
transposition
or error in DNA replication
ACT IN THE SAME WAY
Slide 3:
RFLP Technique
DNA isolation (text file)
Digestion of the DNA with a restriction enzyme
Separation of the restricted fragments by agarose gel electrophoresis/PAGE
Southern Blotting
Detection of individual restriction fragments
by nucleic acid hybridization
with labeled cloned probes,
Scoring of RFLPs by direct observation of autoradiograms. RFLP ANIMATED