PCR Based Mol Markers

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Slide 1: 

PCR-Based Molecular Markers PCR is a procedure for the in vitro enzymatic amplification of a specific segment of DNA (Mullis and Faloona, 1987).

Slide 2: 

Understanding PCR Click on the picture to see its functioning Thermocycler Example 1: DNA Detective Example 2: DNA Sequencing

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…….AGCTAATCTAGAGAGAGAGAGAGCTAGCTGATTCAACTAC……. TCGA PCR AACTC …….TCGATTAGATCTCTCTCTCTCTCGATCGACTATG TTGAG……. New strand New strand PCR is a procedure for the in vitro enzymatic amplification of a specific segment of DNA PCR Animated 2 4 8 16 32 64 128 256 512 1024 2048 ………… No. of Copies 95oC 35-60oC 72oC

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