MAS assisted Back Crossing

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x B A H B x x X y X y x x x X y Parent 1 Agriculturally important variety X y Markers Parent 1 Parent 1 BC1F1 generation The markers, x and y, are used to select the progeny which have the desired alleles Parent 2: Donor variety with tolerance QTL between markers x and y on chromosome A Backcross the F1 with Parent 1 several times and use markers to select for region of genome containing tolerance genes, followed by selfing of selected plants to obtain homozygote. Figure 3: Schematic diagram for the Marker-Assisted Back Crossing / Selection of desirable traits, such as drought tolerance After Catherine and Yadav (2002) F1 generation