Comparison of molecular markers

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Slide 1: 

Extraordinary abundance RFLP RFLP RFLP RFLP SSR SNP Arbitrary Example RFLP = 5 forms SSR = 21 forms SNP = 63 forms Observations 1 SNP / 100-300bp dbSNP = 8,03,557 (Aug 2000) Human genome = 3,00,000 (public db) = + 5,00,000 (private sector) Estimate SNPs in human genome = 5,00,000 to 1,000,000 Density = 1 SNP / 3-5 kb Human genome = 30,000 SNPs are sufficient to scan it © A.K. Chhabra

Slide 2: 

euchromatin heterochromatin SNPs may be found both in the non-repetitive coding or regulatory sequences and I the repetitive non-coding sequences If coding sequences May or may not determine The mutant phenotype But would show 100% Co-segregation with the trait. Very useful for MAS And gene isolation If found in the proximity of Coding sequences Association with the trait may be present but will always be less Than 100% If away from the gene Of no use © A.K. Chhabra