TRANSGENIC-GENETIC MALE STERILITY

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TRANSGENIC-GENETIC MALE STERILITY : 

TRANSGENIC-GENETIC MALE STERILITY A.K. Chhabra

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Barnase gene…produces RNAse Barnase gene…produces RNAse TA29 Barnase gene Tissue specific (tapetum layer) promoter Herbicide Resistance (Phosphinothricin) (Gene construct) Endothecium Middle layer Tapetum Bacillus amyloliquefaciens Tapetum degenerates PMCs/pollen become sterile BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE © A.K. Chhabra 2007 PMCs

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Tapetum persistant PMCs/pollen are fertile Barnase gene…produces RNAse Barstar gene TA29 Barnase gene Tissue specific (tapetum layer) promoter Herbicide Resistance (Phosphinothricin) (Gene construct) Inhibits RNAse Endothecium Middle layer Tapetum Bacillus amyloliquefaciens BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE © A.K. Chhabra 2007 PMCs

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Barnase gene…produces RNAse Barstar gene (fertility restorer) TA29 Barnase gene Tissue specific (tapetum layer) promoter Herbicide Resistance (Phosphinothricin) (Gene construct) Inhibits RNAse Endothecium Middle layer Tapetum Bacillus amyloliquefaciens Tapetum degenerates PMCs/pollen become sterile Tapetum persistant PMCs/pollen are fertile BARNASE-BARSTAR SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE Dominant male sterility Barstar-Barnase interaction (binding) © A.K. Chhabra 2007 PMCs

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