Gene Transfer through Ti Plasmid

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Animated Gene Transfer through Ti Plasmid

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Presentation Transcript

Slide 1: 

Agrobacteium tumefaciens Infection Process

Slide 2: 

Vir-gene is removed from Ti plasmid T-DNA gets broken down from Ti plasmid and integrated into host chromosme If any other gene is also linked with this T-DNA, would also be transferred to host cell. Tumor is not formed because vir-gene is absent. Antibiotic resistance gene is also linked with the T-DNA, so the transformed cells would grow on the medium having that particular antibiotic. Agrobacterium tumefaciens PLANT HOST CELL What happens before crown gall formation?

Slide 3: 

Agrobacterium tumefaciens PLANT HOST CELL T-DNA F-gene Anti-biotic Resistance gene T-DNA + Ab-rest. + f-gene are integrated

Slide 4: 

Agrobacterium tumefaciens PLANT HOST CELL T-DNA + Ab-rest. + f-gene are integrated to host cell chromosome Grow them on Growth medium having Ab

Slide 5: 

Agrobacterium tumefaciens PLANT HOST CELL Transformed cells would grow into transgenic plants