Presentation Transcript
Slide 1:Agrobacteium tumefaciens Infection Process
Slide 2:Vir-gene is removed from Ti plasmid T-DNA gets broken down from Ti plasmid
and integrated into host chromosme If any other gene is also linked with this T-DNA, would also be transferred to host cell.
Tumor is not formed because vir-gene is absent.
Antibiotic resistance gene is also linked with the T-DNA, so the transformed cells would grow on the medium having that particular antibiotic. Agrobacterium tumefaciens PLANT HOST CELL What happens before crown gall formation?
Slide 3:Agrobacterium tumefaciens PLANT HOST CELL T-DNA F-gene Anti-biotic
Resistance
gene T-DNA + Ab-rest. + f-gene are integrated
Slide 4:Agrobacterium tumefaciens PLANT HOST CELL T-DNA + Ab-rest. + f-gene are integrated to
host cell chromosome Grow them on Growth medium having Ab
Slide 5:Agrobacterium tumefaciens PLANT HOST CELL Transformed cells would grow into transgenic plants