Slide 2:
Male Fertile Male Sterile Which of the molecular
markers to be used in MAS ? Mapping Population MAS BSA
Slide 3:
BULK SEGREGANT ANALYSIS X F1 F2 R S R S S I R I R I S I R R R R I I I S R S R S R S R
Slide 6:
Isolate
DNA Bulk DNA of
10 resistant
plants Bulk DNA of
10 susceptible
plants BR BS
Slide 7:
SSR 1 / AFLP 1 SSR 1 / AFLP 1 SSR 1 / AFLP 1 SSR 1 / AFLP 1 R BR S BS R BR S BS R BR S BS R BR S BS Look
For
Co-
Seg.
marker INDIVIDUAL PLANT
DNA SAMPLES,
REMAINING
40R AND 30S
Slide 8:
R BR S BS 40 resistant plants 30 susceptible plants SSR 1 / AFLP 1
Perfect co-segregating marker
Slide 9:
R BR S BS 40 resistant plants 30 susceptible plants SSR 1 / AFLP 1
One Perfect and one closely
co-segregating marker
Slide 10:
Figure 2. : Marker Assisted Selection (Bulk Segregant Analysis) for resistance against Barley Yellow Dwarf Virus Using AFLP II PCR primers. R=resistant, S=Susceptible Resistant Susceptible F2 Population Marker-assisted Selection