Slide 2:
Barnase gene…produces RNAse Barnase gene…produces RNAse TA29 Barnase gene Tissue specific
(tapetum layer)
promoter Herbicide
Resistance
(Phosphinothricin) (Gene construct) Endothecium Middle layer Tapetum Bacillus
amyloliquefaciens Tapetum
degenerates
PMCs/pollen
become
sterile BARNASE-BARSTAR
SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE © A.K. Chhabra 2007 PMCs
Slide 3:
Tapetum
persistant
PMCs/pollen
are
fertile Barnase gene…produces RNAse Barstar gene TA29 Barnase gene Tissue specific
(tapetum layer)
promoter Herbicide
Resistance
(Phosphinothricin) (Gene construct) Inhibits RNAse Endothecium Middle layer Tapetum Bacillus
amyloliquefaciens BARNASE-BARSTAR
SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE © A.K. Chhabra 2007 PMCs
Slide 4:
Barnase gene…produces RNAse Barstar gene
(fertility restorer) TA29 Barnase gene Tissue specific
(tapetum layer)
promoter Herbicide
Resistance
(Phosphinothricin) (Gene construct) Inhibits RNAse Endothecium Middle layer Tapetum Bacillus
amyloliquefaciens Tapetum
degenerates
PMCs/pollen
become
sterile Tapetum
persistant
PMCs/pollen
are
fertile BARNASE-BARSTAR
SYSTEM TRANSGENIC GENETIC MALE STERILITY PRINCIPLE Dominant male sterility Barstar-Barnase interaction (binding) © A.K. Chhabra 2007 PMCs