Peptide Microarray Technology From LC Sciences

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Slide 1:

Peptide Microarray Technology From LC Sciences

Slide 2:

Microfluidic Array Platform μParaflo ® Microfluidics Chip 10 µl total volume 4000 features 270 pl / reaction chamber uniform flow rate

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The Core: 3 Levels of Flexibility Chemistry Digital Photolithography Microfluidics

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Chemistry Does not require electrochemical surface Nor specialty monomers Standard t-Boc chemistry allows use of wide array of (non-regular) building blocks The Core: 3 Levels of Flexibility

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3 Levels of Flexibility Digital Photolithography custom sequences on demand synthesis

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3 Levels of Flexibility Microfluidics multiple washing steps T-variation using the same chip, keeping all other parameters constant

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Si Substrate Cover Glass Distribution Channel Fluid Channel Reaction Chamber μParaflo ® Microfluidics Chip Light Beam Photogenerated Reagent

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DNA DNA array OligoMix ® Modified Nucleotides miRNA array ncRNA array Peptides Kinase array Protein binding array Custom arrays Probe Diversity

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Microfluidics vs. Spotted Arrays - Raw Data

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Microfluidics vs. Spotted Arrays - Raw Data

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Spot Uniformity Enhanced Mass Transfer: improving sensitivity High Reproducibility Closed System: no dye “bleaching” Incremental Stringency Wash: higher specificity Stable for years Microfluidics vs. Spotted Arrays

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LC Control Cy3 miRNA LC Control Cy5 miRNA LC Control Ratio miRNA Pseudo color images Biomarker Discovery / Profiling: Differential Expression - One Dual Sample Chip control (Cy3) vs treated (Cy5) Cy3/Cy5 ratio