logging in or signing up GPCr detail chandni.dave Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 150 Category: Science & Tech.. License: All Rights Reserved Like it (0) Dislike it (0) Added: September 23, 2012 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript PowerPoint Presentation: Last Class: A. intracellular vesicle traffic 1. ER to golgi 2. endocytosis, exocytosis B. Signaling Transduction 1. Ligand Receptor Interactions, 2. Intracellular signaling molecules, 3. molecule switches: phosphorylation and GTP binding, 4. Signaling integration, 5. complex formation, 6. Signaling amplification, 7. Signaling desensitizationPowerPoint Presentation: Cell Communication Different Receptor Types Ion channel-linked receptors *GPCR signaling *Enzyme linked receptorsPowerPoint Presentation: G Protein Coupled Receptors (GPCR)PowerPoint Presentation: A G-Protein-Coupled Receptor Or G Protein-linked Receptor 7 transmembrane domainsPowerPoint Presentation: The disassembly of G-Protein upon stimulation Spontaneous deactivation is very fast, in minutes. However, with the help of RGS (regulator of G protein signaling, a GAP for a unit), signals can be shut off even fasterPowerPoint Presentation: The Activation cycle of G-ProteinPowerPoint Presentation: GPCR Signaling: cAMPPowerPoint Presentation: The visualization of cAMP in nerve cells GPCR->Gs->adenylyl cyclase->cAMP GiPowerPoint Presentation: cAMP cycle: GPCR->Gs->adenylyl cyclase->cAMP Cyclic AMP phosphodiesterase breaks down cAMP to 5 ’ -AMPPowerPoint Presentation: The function of cAMP Targeting PKA (cyclic-AMP-dependent protein kinase A)PowerPoint Presentation: The Whole Signaling Network related to cAMPPowerPoint Presentation: Terminology: CRE(cyclic AMP response element); CREB: CRE binding protein; CBP: CREB binding proteinPowerPoint Presentation: GPCR Signaling: CalciumPowerPoint Presentation: Three Types of Inositol phospholipids PI, PI(4)P, PI(4,5)P2PowerPoint Presentation: Phospholipase C- b (PLC- b ) Produces DAG (diacylglycerol) and IP3 (inositol 1,4,5-trisphosphate (IP3)) Gq->PLC- bPowerPoint Presentation: Gq signaling pathways and CalciumPowerPoint Presentation: Fertilization of an egg by a sperm triggering an increase in cytosolic Calcium 3 major types of calcium channels: Voltage dependent Ca channels on plasma membrane IP3-gated Ca release channels on ER membrane Ryanodine receptor on ER membranePowerPoint Presentation: Calcium uptake and deprivation Na/Ca exchanger on plasma membrane, 2. Ca pump on ER membrane, 3. Ca binding molecules, 4. Ca pump on MitochondiaPowerPoint Presentation: Calcium Frequency encoding signaling strength Local Ca blips, sparks, puffs, reflecting local opening of individual channels in ER, strong local signal induces global activity, the elevated Calcium trigger calcium deprivation systemPowerPoint Presentation: Targeting molecules for Calcium Calcium binding protein CalmodulinPowerPoint Presentation: Ca 2+ /calmodulin dependent protein kinase (CaM-kinase) Memory function: 1. calmodulin dissociate after 10 sec of low calcium level; 2. remain active after calmodulin dissociationPowerPoint Presentation: Ca 2+ /calmodulin dependent protein kinase (CaM-kinase) Frequency decoder of Calcium oscillation High frequence, CaM-kinase does not return to basal level before the second wave of activation startsPowerPoint Presentation: Desensitization of GPCR 1. Inhibitory structural alteration of receptor; 2. receptor internalization; 3. receptor degration GRK (G protein-linked receptor kinase) Arrestin takes to clathrin-coated pits and degradationPowerPoint Presentation: GPCR Signaling Summary 1. G-protein types 2. cAMP and Calcium signaling pathways 3. desensitizationPowerPoint Presentation: Enzyme-Linked Cell Surface Receptors *Receptor Tyrosine Kinase *Tyrosine kinase associated receptors *Receptor-like tyrosine phosphatase *Receptor serine/threonine kinase Receptor guanylyl cyclase Histidine like associated receptorPowerPoint Presentation: Receptor Tyrosine Kinases (RTKs)PowerPoint Presentation: Seven subfamilies of receptor tyrosine kinasesPowerPoint Presentation: Three ways in which signaling proteins can cross-link receptor chains 1. dimer, 2. monomer but brought together by proteoglycan, 3. cluster on membranePowerPoint Presentation: The importance of receptor oligomerizationPowerPoint Presentation: The docking of signaling molecules at RTKPowerPoint Presentation: The binding of SH2-containing intracellular signaling proteins to an activated PDGF receptorPowerPoint Presentation: The structural view of SH2 domainPowerPoint Presentation: RTK Signaling: Ras PathwayPowerPoint Presentation: The regulation of Ras activity, a famous downstream molecule of RTK responsible for cancer developmentPowerPoint Presentation: The activation of Ras by RTK signalingPowerPoint Presentation: The MAP-kinase regulated by RasPowerPoint Presentation: RTK Signaling: PI3K PathwayPowerPoint Presentation: The inositol phospholipids generated by PI3KPowerPoint Presentation: The recruitment of signaling molecules with PH domains to the plasma membrane during B cell activation One PI3K pathway PH domain: pleckstrin homology domainPowerPoint Presentation: Another PI3K pathway to regulate cell survivalPowerPoint Presentation: Another PI3K pathway to regulate cell migration PI3K->PIP3->GEF->Rac->Wave->Arp2/3->Actin polymerization Movie: chemotaxis, PI3K and cell migrationPowerPoint Presentation: Intracellular Signaling Pathways activated by RTKs and GPCRsPowerPoint Presentation: Tyrosine kinase associated receptors Integrins: cell-extracellular matrix adhesion Binding to Src and FAKPowerPoint Presentation: Receptorlike tyrosine phosphatases Intracellular protein and receptorPowerPoint Presentation: Receptor Serine/threonine kinase Transforming growth factor (TGF-b) and Smad signaling pathwayPowerPoint Presentation: Protein kinase SummaryPowerPoint Presentation: Enzyme-linked Receptor Signaling Summary 1. receptor types 2. RTK and its signaling: Ras and PI3K 3. Tyrosine kinase associated receptors and Receptor-like tyrosine phosphatase 4. Receptor serine/threonine kinase, TGF- b and SmadPowerPoint Presentation: Other Signaling Pathways Proteolysis mediatedPowerPoint Presentation: Notch and Delta interaction Lateral inhibitionPowerPoint Presentation: The inhibitory pathway of Notch Proteolysis-mediatedPowerPoint Presentation: The inhibitory pathway of Notch Proteolysis-mediatedPowerPoint Presentation: Wnt Signaling pathway LRP: LDL-receptor-related protein; GSK-3 b : glycogen synthase kinase-3 b; APC: adenomatous polyposis coli;PowerPoint Presentation: NF-KB pathwayPowerPoint Presentation: Summary 1. GPCR signaling: PKA and Calcium 2. Enzyme-linked Receptor signaling: RTK->Ras and PI3K 3. Proteolysis-mediated signaling pathways You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.