Review Ch 1-4

Views:
 
Category: Entertainment
     
 

Presentation Description

No description available.

Comments

Presentation Transcript

Sept. 9 Announcements : 

Sept. 9 Announcements Today: review quiz, review lab questions/calculations, Microbiology Jeopardy, case discussions Virology, covered as a unit at the end of semester, to keep on same time line as other classes for Review Sessions Sept 12 (Sunday): Group Tutoring 6:30, Room 206 Kivett, Covering Chapter 3 Sept 14: Read Chapter 6 prior to Class Sept. 16 (Thurs): EXAM Ch 1, 2, 3, and 4

Quiz 2 : 

Quiz 2 Archeabacteria differ from Bacteria in that Archeabacteria: Have no petidoglycan in their cell walls Are more likely to be found in extreme temperature and salt environments Both a. and b. None of the above

Quiz 2 : 

Quiz 2 Archeabacteria differ from Bacteria in that Archeabacteria: Have no peptidoglycan in their cell walls Are more likely to be found in extreme temperature and salt environments Both a. and b. None of the above

Quiz 2 : 

Quiz 2 Which of the following can be seen easily on a dry mount slide through a good light microscope using a 100X oil immersion lens? Protozoa, bacteria, mycoplasmas White blood cells, protozoa, bacteria White blood cells, bacteria, viruses Red blood cells, protozoa, prions

Quiz 2 : 

Quiz 2 Which of the following can be seen easily on a dry mount slide through a good light microscope using a 100X oil immersion lens? Protozoa, bacteria, mycoplasmas White blood cells, protozoa, bacteria White blood cells, bacteria, viruses Red blood cells, protozoa, prions

Quiz 2 : 

Quiz 2 Van Leeuwenhoek is famous for: Development of his microscope and describing the first microorganisms Experiments that disproved the theory of Spontaneous Generation of microorganisms Describing four postulates that prove the causative agent of a disease None of the above

Quiz 2 : 

Quiz 2 An oil immersion lens increases the clarity of the object being viewed because: Oil has the same refractive index as air Oil has the same refractive index as glass Oil is viscous and fills the space between the slide and lens easily None of the above

Quiz 2 : 

Quiz 2 Gram negative bacteria have which of the following characteristics: a. A thick layer of peptidoglycan in their cell wall b. An outer membrane and a plasma membrane c. A thin layer of peptidoglycan in their cell wall d. Both a. and b. e. Both b. and c.

Quiz 2 : 

Quiz 2 The method of action of Penicillin is: Prevents synthesis of new peptidoglycan Kills mitochondria Disrupts nuclear membranes None of the above

Quiz 2 : 

Quiz 2 Which is not a function of the bacterial cell wall? Movement of nutrients Cell movement Rigidity Adherence

Quiz 2 : 

Quiz 2 The microscopes we use in Microbiology Lab are: Dark-field Compound Light Microscopes Phase Contrast Light Microscopes Bright-field Compound Light Microscopes None of the above

Quiz 2 : 

Quiz 2 Resolution is the ability of a lens to separate or distinguish between small objects that look very similar. True False

Quiz 2 : 

Quiz 2 The image from a scanning electron microscope generally shows the inside detail of single cells, while a transmission electron micrograph shows the outer surface of single cells. True False

Lab Questions : 

Lab Questions

Calculating amount of chemical needed for a specific concentration : 

Calculating amount of chemical needed for a specific concentration X% solution = X g/100 ml XM solution = XM/L Prepare 500ml of a 2%KCl solution Prepare 400ml of a 0.3 M KCl solution Molecular weight of KCl is 74.58

Calculating amount of chemical needed for a specific concentration : 

Calculating amount of chemical needed for a specific concentration X% solution = X g/100 ml XM solution = XM/L Prepare 500 ml of a 2% KCl solution a. 2% solution = 2 g/100ml b. Multiply by 5 to get 500 ml = 10g KCl/500 ml

Calculating amount of chemical needed for a specific concentration : 

Calculating amount of chemical needed for a specific concentration X% solution = X g/100 ml XM solution = XMW/L 2. Prepare 400ml of a 0.3 M KCl solution Molecular Weight of KCl is 74.58 0.3M KCl = (0.3)(74.58)/1L 0.3 (74.58)g= 22.4/1L For 400ml >>> (0.4L) 22.4g = 8.96g/0.4L (0.4 L) (1L)

Practice Calculations : 

Practice Calculations Molecular weights: Sucrose: 342.8 gm Salt (NaCl): 58.4 gm Potassium Chloride (KCl): 74.55 gm How would you prepare 200 ml of a 7% salt solution? How would you prepare 200 ml of a 0.3% sucrose solution? How would you prepare 500 ml of a 2% KCl solution? How would you prepare 200 ml of a 7 M salt solution? How would you prepare 200 ml of a 0.3M sucrose solution? How would you prepare 500 ml of a 2M KCl solution?

Organic Macromolecules required by all Living Organisms : 

Organic Macromolecules required by all Living Organisms Proteins (amino acids), Carbohydrates (sugars), Lipids (phospholipids), Nucleic Acids Based on what you know about Chemistry, what are the elementary nutritional requirements of microbes (what elements do they need to survive)?

Organic Macromolecules required by all Living Organisms : 

Organic Macromolecules required by all Living Organisms Proteins (amino acids), Carbohydrates (sugars), Lipids (phospholipids), Nucleic Acids Based on what you know about Chemistry, what are the elementary nutritional requirements of microbes (what elements do they need to survive)? Carbon, hydrogen, oxygen nitrogen, phosphate and special minerals

What is missing in this media? : 

What is missing in this media? Defined media containing: K2HPO4 NH4Cl MgSO4-7H20 FeCl3-6H20 Agar

Why do we autoclave nutrient agar, especially after pouring slants? : 

Why do we autoclave nutrient agar, especially after pouring slants? There are at least 4 reasons

Why do we autoclave nutrient agar, especially after pouring slants? : 

Why do we autoclave nutrient agar, especially after pouring slants? Heat allows agar to dissolve into solution Heat sterilizes agar Heat sterilizes interior and exterior of glass tube Heat sterilizes interior and exterior of cap

Hand washing and bacteria : 

Hand washing and bacteria Why are there bacteria in every quadrant? unwashed washed Super washed Alcohol unwashed

Hand washing and bacteria : 

Hand washing and bacteria Why are there bacteria in every quadrant? unwashed washed Super washed Alcohol Bacteria loosened in washing Skin cells with bacteria loosened while washing Did not wash/sanitize well Air contamination

Selective vs. Differential Media : 

Selective vs. Differential Media Selective: only certain types of bacteria can grow Example: MacConkey Agar, only Gram negative organisms (like E. coli) can grow on this agar because it has crystal violet and bile salts Differential: many species of bacteria can grow, but they can be distinguished based on differences Example: MacConkey Agar, lactose fermenters like coliform bacteria turn red, while non-coliforms remain transparent

How could you improve your experimental design? : 

How could you improve your experimental design? Better or additional controls More repetitions of each sample collected from different locations Larger area swabbed Consistent amount of area swabbed

Microbiology Jeopardy : 

Microbiology Jeopardy Divide into 4 teams Pick a TEAM LEADER, SCORE KEEPER, and TEAM NAME Pass the “buzzer” so that each team member takes a turn to “buzz in” and answer a question, but the whole team gets 30 seconds to discuss it first If the first team to “buzz in” answers is correctly They select the next category and value If the first team’s answer is incorrect The next team clockwise gets to try to answer

Microbiology Jeopardy : 

Microbiology Jeopardy All answers must be given in the form of a question: If the “clue” or answer was: Man who developed the Gram stain Your response would be: Who is Gram?

authorStream Live Help