logging in or signing up AI SWINE IN NICARAGUA bolcott Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 745 Category: Science & Tech.. License: All Rights Reserved Like it (0) Dislike it (0) Added: September 03, 2007 This Presentation is Public Favorites: 0 Presentation Description No description available Comments Posting comment... By: tuandatdvm (10 month(s) ago) Dear aurthor, I see your documents is very helpful for me in my work please help me download these files. thank you, Saving..... Post Reply Close Saving..... Edit Comment Close By: tuandatdvm (12 month(s) ago) Dear admin, This file is very good for me. Please help allow me to download it Thank you, Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript Artificial Insemination in Swine: Artificial Insemination in Swine B. Olcott DVM,MS,MBA Winrock Nicaragua 2007Advantages of Artificial Insemination: Advantages of Artificial InseminationComparative Cost Of AI: Comparative Cost Of AIArtificial Insemination Kit: Artificial Insemination KitBe Ready!: Be Ready! Have insulated collection cup warm and clean until time to collect Extenders and other lab materials (slides) need to be pre-mixed and warmed to appropriate temperaturesCollecting Semen: Collecting Semen Place a sterile disposable bag inside the container. Semen Straining: Semen Straining Place a double layer of sterile cheese cloth over the cup. Boil the cloth between collections. Secure with rubber bands. Collecting Semen: Collecting Semen Warming cabinets keep all supplies at a uniform temperature of 35oC. Distilled water Slides Extender Glassware StainsCollecting Semen: Collecting Semen Collection areas should be clean and quiet. Flooring should be non slippery. Escape hatches should be provided for personnel. Collecting Semen: Collecting Semen Training boars Young boars will usually respond well to the collection dummy. Older boars are more difficult. Urine from sows in heat is a great aide. Collecting Semen: Collecting Semen Familiarize the boar with contact on all parts of his body. Be patient.Collecting Semen: Collecting Semen Let the boar explore his environment. Collecting Semen: Collecting Semen Once a boar is trained collecting is a one person job. Grasp the tip of the boars penis firmly with poly vinyl NOT LATEX Gloves. Discard the first part of ejaculate which is the clear fraction.Collecting Semen: Collecting Semen Collect the milky white sperm-rich portion. The final portion is the clear and gel fraction and not collected.Collecting Semen: Collecting Semen There may be another sperm rich fraction than can be collected. Make sure the penis remains safe from damage from the dummy sow.Collecting Semen: Collecting Semen Be patient. He will need to finish to avoid frustration. He may take 5-15 minutes Avoid pressure on the shaft, this may distract him and make him stop. Keep pressure applied to tip only!Collecting Semen: Collecting Semen Remove the filter and discard. The semen should be extended within 15 minutes of collection. Keep the sample away from cool temperatures and light!!Semen Evaluation: Semen Evaluation Weigh the semen first. Record the volume collected. Evaluate color, thickness, and smell.Semen Motility Evaluation: Semen Motility Evaluation Estimate motility within 10-15 minutes of collection. Slides and cover slips warmed to 35oC Extender warmed to 33oC Add 9.5 mls of extender to 0.5 mls of semen. (1:19 dilution). Place 2 drops on a warm slide and cover with warm cover slip. Examine at 400x. Sperm Morphology: Sperm MorphologySemen Morphology: Morphology Stain Trypan Blue Eosin/Nigrosin solution 7 drops stain to 3 drops semen Must be warm or false readings!! Let sit for 5 minutes in water bath before reading. Semen MorphologyExtending Semen: Extending Semen Dose = 3-4 x 109 Hemocytometer Spectrophotometer Estimate Skim milk: 1:4 Milk: 1:7 Cream: 1:10Sperm Concentration: Sperm Concentration Estimate by appearance Hand count with hemocytometer Electronic count with photometer Minimum of 2 x 109 viable sperm in one dose (70 ml) Maximum of 4.5 x 109 viable sperm in one dose (70 ml)Hemocytometer: HemocytometerHemocytometer: HemocytometerExtenders: Extenders What do extenders do? Dilute semen Provide buffers Provide nutrients Provide antibacterials to prevent infectionExtenders: Extenders Long term extenders: Shelf life of 5-7 days Difficult to prepare Expensive Short term extenders: Shelf life of 3 days Inexpensive Easy to makeGuidelines to Extending Semen: (Amount of extender to add): Guidelines to Extending Semen: (Amount of extender to add)Extending Semen: Extending SemenAdding Extender to Semen: Adding Extender to SemenExtending Semen by Eye: Extending Semen by EyeRecords: Records Label EACH dose with boar ID, date, concentration, etc.Storing Semen: Storing Semen Always store semen between 15-20oC. Cool warm semen over a period of 2-3 hours. Never store semen in a refrigerator (2o-8oC). Never store semen at a temperature greater than 20oC. Invert (roll) doses 2x daily during storage. Inseminating the Sow: Inseminating the SowArtificial InseminationSpirettes: Artificial Insemination Spirettes Insemination : Insemination AI Catheter Placement: AI Catheter Placement Artificial Insemination: Artificial Insemination Spirette is inserted at an angle of 30o. This will prevent catheterization of the bladder. Spirette should advance easily to the cervix. A hymen may be present in gilts. Artificial Insemination: Artificial Insemination Rotate the spirette counterclockwise! This will lock it in place in the cervix. Turn the spirette until it won’t turn any more.Artificial Insemination: Artificial Insemination Spirette locked in placeArtificial Insemination: Artificial Insemination Back pack set Sows in heat with proper stimulation will draw semen from the vial intra uterinely. Once semen is drawn in, leave catheter in place for 2 minutes to minimize back flow. You do not have the permission to view this presentation. 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AI SWINE IN NICARAGUA bolcott Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 745 Category: Science & Tech.. License: All Rights Reserved Like it (0) Dislike it (0) Added: September 03, 2007 This Presentation is Public Favorites: 0 Presentation Description No description available Comments Posting comment... By: tuandatdvm (10 month(s) ago) Dear aurthor, I see your documents is very helpful for me in my work please help me download these files. thank you, Saving..... Post Reply Close Saving..... Edit Comment Close By: tuandatdvm (12 month(s) ago) Dear admin, This file is very good for me. Please help allow me to download it Thank you, Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript Artificial Insemination in Swine: Artificial Insemination in Swine B. Olcott DVM,MS,MBA Winrock Nicaragua 2007Advantages of Artificial Insemination: Advantages of Artificial InseminationComparative Cost Of AI: Comparative Cost Of AIArtificial Insemination Kit: Artificial Insemination KitBe Ready!: Be Ready! Have insulated collection cup warm and clean until time to collect Extenders and other lab materials (slides) need to be pre-mixed and warmed to appropriate temperaturesCollecting Semen: Collecting Semen Place a sterile disposable bag inside the container. Semen Straining: Semen Straining Place a double layer of sterile cheese cloth over the cup. Boil the cloth between collections. Secure with rubber bands. Collecting Semen: Collecting Semen Warming cabinets keep all supplies at a uniform temperature of 35oC. Distilled water Slides Extender Glassware StainsCollecting Semen: Collecting Semen Collection areas should be clean and quiet. Flooring should be non slippery. Escape hatches should be provided for personnel. Collecting Semen: Collecting Semen Training boars Young boars will usually respond well to the collection dummy. Older boars are more difficult. Urine from sows in heat is a great aide. Collecting Semen: Collecting Semen Familiarize the boar with contact on all parts of his body. Be patient.Collecting Semen: Collecting Semen Let the boar explore his environment. Collecting Semen: Collecting Semen Once a boar is trained collecting is a one person job. Grasp the tip of the boars penis firmly with poly vinyl NOT LATEX Gloves. Discard the first part of ejaculate which is the clear fraction.Collecting Semen: Collecting Semen Collect the milky white sperm-rich portion. The final portion is the clear and gel fraction and not collected.Collecting Semen: Collecting Semen There may be another sperm rich fraction than can be collected. Make sure the penis remains safe from damage from the dummy sow.Collecting Semen: Collecting Semen Be patient. He will need to finish to avoid frustration. He may take 5-15 minutes Avoid pressure on the shaft, this may distract him and make him stop. Keep pressure applied to tip only!Collecting Semen: Collecting Semen Remove the filter and discard. The semen should be extended within 15 minutes of collection. Keep the sample away from cool temperatures and light!!Semen Evaluation: Semen Evaluation Weigh the semen first. Record the volume collected. Evaluate color, thickness, and smell.Semen Motility Evaluation: Semen Motility Evaluation Estimate motility within 10-15 minutes of collection. Slides and cover slips warmed to 35oC Extender warmed to 33oC Add 9.5 mls of extender to 0.5 mls of semen. (1:19 dilution). Place 2 drops on a warm slide and cover with warm cover slip. Examine at 400x. Sperm Morphology: Sperm MorphologySemen Morphology: Morphology Stain Trypan Blue Eosin/Nigrosin solution 7 drops stain to 3 drops semen Must be warm or false readings!! Let sit for 5 minutes in water bath before reading. Semen MorphologyExtending Semen: Extending Semen Dose = 3-4 x 109 Hemocytometer Spectrophotometer Estimate Skim milk: 1:4 Milk: 1:7 Cream: 1:10Sperm Concentration: Sperm Concentration Estimate by appearance Hand count with hemocytometer Electronic count with photometer Minimum of 2 x 109 viable sperm in one dose (70 ml) Maximum of 4.5 x 109 viable sperm in one dose (70 ml)Hemocytometer: HemocytometerHemocytometer: HemocytometerExtenders: Extenders What do extenders do? Dilute semen Provide buffers Provide nutrients Provide antibacterials to prevent infectionExtenders: Extenders Long term extenders: Shelf life of 5-7 days Difficult to prepare Expensive Short term extenders: Shelf life of 3 days Inexpensive Easy to makeGuidelines to Extending Semen: (Amount of extender to add): Guidelines to Extending Semen: (Amount of extender to add)Extending Semen: Extending SemenAdding Extender to Semen: Adding Extender to SemenExtending Semen by Eye: Extending Semen by EyeRecords: Records Label EACH dose with boar ID, date, concentration, etc.Storing Semen: Storing Semen Always store semen between 15-20oC. Cool warm semen over a period of 2-3 hours. Never store semen in a refrigerator (2o-8oC). Never store semen at a temperature greater than 20oC. Invert (roll) doses 2x daily during storage. Inseminating the Sow: Inseminating the SowArtificial InseminationSpirettes: Artificial Insemination Spirettes Insemination : Insemination AI Catheter Placement: AI Catheter Placement Artificial Insemination: Artificial Insemination Spirette is inserted at an angle of 30o. This will prevent catheterization of the bladder. Spirette should advance easily to the cervix. A hymen may be present in gilts. Artificial Insemination: Artificial Insemination Rotate the spirette counterclockwise! This will lock it in place in the cervix. Turn the spirette until it won’t turn any more.Artificial Insemination: Artificial Insemination Spirette locked in placeArtificial Insemination: Artificial Insemination Back pack set Sows in heat with proper stimulation will draw semen from the vial intra uterinely. Once semen is drawn in, leave catheter in place for 2 minutes to minimize back flow.