logging in or signing up AI Equipment and Semen bolcott Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 1198 Category: Science & Tech.. License: All Rights Reserved Like it (2) Dislike it (0) Added: September 03, 2007 This Presentation is Public Favorites: 0 Presentation Description No description available Comments Posting comment... Premium member Presentation Transcript Artificial Insemination Equipment and Semen Collection: Artificial Insemination Equipment and Semen Collection Bruce Olcott DVM,MS,MBA Winrock 2007Equipment List: Equipment ListCito Automatic Thaw: Cito Automatic ThawTemperature: Temperature Cito thaw bath has a red light/green light that monitors temperature. Thermos thaw jars require warm water. Cito Thaw Bath: Cito Thaw Bath Green light means good to go. Red light means not warm enough. Check water temperature monthly and recalibrate if necessary. Straw Thermos: Straw Thermos Warm water is added to the thermos. Temperature must be monitored. Storage: Storage Sheaths and guns must be kept clean at all times. Store in an airtight canister. Alcohol disinfect the gun after use. Discard sheaths. Storage: Storage Stainless steel case can be disinfected between farms. Air tight to keep dust out. ‘O’ Ring guns: ‘O’ Ring guns Uses a non-split sheath. “O” ring holds the sheath on to the gun. Loading the gun: Loading the gun Straw Sheath GunLoading the gun: Loading the gun Straw goes into the sheath and locks in the adapter. Loading the Gun: Loading the Gun Gun goes inside sheath. To the tip of the straw. Semen Handling: Semen Handling Frozen straws are removed from the tank with tweezers. This protects you and the straw. Sleeves: Sleeves Disposable sleeves. Sleeves are discarded after every cow. Scissors and Tweezers: Scissors and Tweezers Tweezers to get straw from the tank. Scissors to cut straw. Disinfecting: Disinfecting Clean equipment with water. Disinfect with alcohol. Do not use disinfectants. They will kill sperm. Semen Collection and Processing: Semen Collection and ProcessingSlide18: Live mount with ejaculation into an artificial vagina. Dummies available for: Stallions Boars Bulls Electro – ejaculation Manual massage Epididymal semenSemen Collection: Artificial Vagina Temperature Bull, stallion, ram at 45°C Pressure Friction Lubrication essential Semen CollectionSlide20: Electro-ejaculation Useful if male won’t or can’t mount Poorer quality ejaculate Used in rams and beef bulls Massage Method Stimulate by rectal massage Seminal vesicles Vas deferensSlide21: Extenders include: Nutrients Glucose, fructose Volume expansion Milk, skim-milk, egg yolk Tris Buffers Citrate, Tris Inhibit bacterial growth Antibiotics Cryoprotectant Glycerol Extend then Freeze!Slide22: After collection and extention, semen is loaded into a straw. The straw is placed into a Liquid Nitrogen Tank. Each tank contains a Cane, which consists of Goblets containing the semen straws themselves. Make sure that the correct procedure for freezing semen in a liquid nitrogen is followed.The Semen Tank: The Semen TankStoring semen: Storing semenFrozen Pellets: Frozen PelletsAmpules: AmpulesSlide27: Use the procedures recommended by the semen supplier!!! 35°C water for 30 - 60 seconds Ice water for 3 minutes Pocket thaw Be very careful when removing straw from Nitrogen Tank Thawing You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
AI Equipment and Semen bolcott Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 1198 Category: Science & Tech.. License: All Rights Reserved Like it (2) Dislike it (0) Added: September 03, 2007 This Presentation is Public Favorites: 0 Presentation Description No description available Comments Posting comment... Premium member Presentation Transcript Artificial Insemination Equipment and Semen Collection: Artificial Insemination Equipment and Semen Collection Bruce Olcott DVM,MS,MBA Winrock 2007Equipment List: Equipment ListCito Automatic Thaw: Cito Automatic ThawTemperature: Temperature Cito thaw bath has a red light/green light that monitors temperature. Thermos thaw jars require warm water. Cito Thaw Bath: Cito Thaw Bath Green light means good to go. Red light means not warm enough. Check water temperature monthly and recalibrate if necessary. Straw Thermos: Straw Thermos Warm water is added to the thermos. Temperature must be monitored. Storage: Storage Sheaths and guns must be kept clean at all times. Store in an airtight canister. Alcohol disinfect the gun after use. Discard sheaths. Storage: Storage Stainless steel case can be disinfected between farms. Air tight to keep dust out. ‘O’ Ring guns: ‘O’ Ring guns Uses a non-split sheath. “O” ring holds the sheath on to the gun. Loading the gun: Loading the gun Straw Sheath GunLoading the gun: Loading the gun Straw goes into the sheath and locks in the adapter. Loading the Gun: Loading the Gun Gun goes inside sheath. To the tip of the straw. Semen Handling: Semen Handling Frozen straws are removed from the tank with tweezers. This protects you and the straw. Sleeves: Sleeves Disposable sleeves. Sleeves are discarded after every cow. Scissors and Tweezers: Scissors and Tweezers Tweezers to get straw from the tank. Scissors to cut straw. Disinfecting: Disinfecting Clean equipment with water. Disinfect with alcohol. Do not use disinfectants. They will kill sperm. Semen Collection and Processing: Semen Collection and ProcessingSlide18: Live mount with ejaculation into an artificial vagina. Dummies available for: Stallions Boars Bulls Electro – ejaculation Manual massage Epididymal semenSemen Collection: Artificial Vagina Temperature Bull, stallion, ram at 45°C Pressure Friction Lubrication essential Semen CollectionSlide20: Electro-ejaculation Useful if male won’t or can’t mount Poorer quality ejaculate Used in rams and beef bulls Massage Method Stimulate by rectal massage Seminal vesicles Vas deferensSlide21: Extenders include: Nutrients Glucose, fructose Volume expansion Milk, skim-milk, egg yolk Tris Buffers Citrate, Tris Inhibit bacterial growth Antibiotics Cryoprotectant Glycerol Extend then Freeze!Slide22: After collection and extention, semen is loaded into a straw. The straw is placed into a Liquid Nitrogen Tank. Each tank contains a Cane, which consists of Goblets containing the semen straws themselves. Make sure that the correct procedure for freezing semen in a liquid nitrogen is followed.The Semen Tank: The Semen TankStoring semen: Storing semenFrozen Pellets: Frozen PelletsAmpules: AmpulesSlide27: Use the procedures recommended by the semen supplier!!! 35°C water for 30 - 60 seconds Ice water for 3 minutes Pocket thaw Be very careful when removing straw from Nitrogen Tank Thawing