logging in or signing up MANUAL BLOOD CELLS COUNT bashirbashir Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 831 Category: Education License: All Rights Reserved Like it (0) Dislike it (0) Added: November 18, 2012 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript MANUAL BLOOD CELLS COUNT: MANUAL BLOOD CELLS COUNT Bashir A. Bashir Mohamed, MSc University of Gizera, 2012Enumerations of RBC count & WBC count: Enumerations of RBC count & WBC countRBC COUNT: RBC COUNT Number of red blood cells per a unit volume ( x million / cu.mm) (x 10 6 / cu.mm) (x 10 6 / mm 3 ) (x 10 6 / µ L) (x 10 12 / L)Reagent of RBC count: Reagent of RBC count Hayam’s solution Sodium Sulphate Sodium Chloride Mercuric Chloride Distilled WaterPowerPoint Presentation: Gower’s solution Sodium Sulphate Glacial acetic acid Distilled water Citrate-formalin solution Tri-sodium Citrate Formalin Normal Saline Reagent of RBC countProperties of the RBC reagents : Properties of the RBC reagents Red cell diluting fluid Anti-coagulant Anti-hemolysis Anti-aggregation Anti-rouleaux Preserve RBC shape Lysis WBCPrinciple of test: Principle of test Whole blood is diluted 1 in 201 in formal citrate reagent which preserve the red cells , leaving the red cells to be counted. Red cells are counted microscopically using an Improved Neubaur ruled counting chamber ( haemocytometer ) and the number of RBCs per liter of blood calculated.WBC COUNT: WBC COUNT Number of white blood cells per a unit volume (x 10 3 / cu.mm) (x 10 3 / mm 3 ) (x 10 3 / µ L) (x 10 9 / L)WBC COUNT; Reagents: WBC COUNT; Reagents WBC diluting fluids 1. Turk’s solution Glacial acetic acid Gential violet Distilled water 2. T isson 2 % acetic acid 3. 1 % hydrochloric acidPrinciple of test: Principle of test Whole blood is diluted 1 in 20 in an acid reagent which haemolyzes the red cells (not the nucleus of nucleated red cells), leaving the white cells to be counted. White cells are counted microscopically using an Improved Neubauer ruled counting chamber ( haemocytometer ) and the number of WBCs per litre of blood calculated.Blood Sample: Blood Sample Use EDTA anticoagulated venous blood. The collection of venous blood. Capillary blood also can be usedHaemocytometer: Haemocytometer Neubauer Chamber Improved Neubauer ChamberCounting Grid Area: Improved Neubauer counting chamber Counting grid Chamber 1 Chamber 2 Counting Grid AreaGrid Area: Grid Area Neubauer ImprovedPowerPoint Presentation: Depth of Chamber = 0.1 mm = 100 m m 1 mm = 1000 m m 1/5 mm = 200 m m 1 4 3 2 A B E D C Counting grid 5Performance: Performance Perform blood cell count within 2 hours of collecting the blood. 1 . Measure 0.38 ml [ WBC ] or 4.0 ml [ RBC ] diluting fluid and dispense it into a small container or tube.PowerPoint Presentation: 2 . Add 20 μ l (0.02 ml, 20 cmm ) of well-mixed anticoagulated venous blood and mix. 3 . Assemble the counting chamber and fill it with well-mixed sample as described previously.PowerPoint Presentation: 4 . Leave the chamber undisturbed for 20 or [ 2 ] minutes. To prevent drying of the fluid, place the chamber in a petri dish or plastic container on dampened tissue or blotting paper and cover with a lid as shownPowerPoint Presentation: 5. Dry the underside of the chamber and place it on the microscope stage. Using the 10 X objective, focus the rulings of the grid and bring the central square of the chamber into view. Change to the 40 X objective and focus the small cell.Important: Important The chamber must be refilled if the sample overfills into the channel beyond the grid or an air bubble forms in the grid area. Correct vs IncorrectSTD Counting Techniques: STD Counting TechniquesPowerPoint Presentation: A cell with most of its lying on the central line is counted only if that line is the upper or left-hand line of the square but not if it is the lower or right hand line of the square. The middle of the three lines defines the square’s boundary (yellow line). All cells within the central square are counted, as well as those between the two inner lines, but not those whose lie between the outer two lines . The Neubauer Counting Chamber Counting Rules6. Count the blood cells in the squares marked W or R as shown: 6 . Count the blood cells in the squares marked W or R as shown 23 R R R R W W W W RPowerPoint Presentation: 7 . Report the number of Blood cell in 1 litre of blood. The actual number of white cell counted in 10 9 and 10 12 for red cell.Calculations: Calculations Formula for manual cell counting: Cell count (per liter) = Cells counted X D.F X 10 6 Volume counted Volume counted = Area (mm 2 ) x depth (mm) D.F = Dilution factor Depth of chamber = 0.1 mmAutomated Cell Counter: Automated Cell CounterPowerPoint Presentation: Thank you You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.