logging in or signing up electron microscope bablipradnya Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 49 Category: Entertainment License: All Rights Reserved Like it (0) Dislike it (0) Added: February 02, 2012 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Electron Microscope: Electron Microscope Dr. Pradnya GajbhiyeElectron Microscope: Electron Microscope • In 1932, invented by E. Ruska et al. • In 1986, Ruska received the Nobel PrizePowerPoint Presentation: Complex, costly Technically difficult to maintain Principle Closely parallel to optical micrroscopeElectron Microscope: Electron Microscope beams of electrons are used to produce images wavelength of electron beam is much shorter than light resulting in much higher resolutionPowerPoint Presentation: Best resolution obtained 3-5 A o i.e. 0.3-0.5 mµSpecimen Preparation: Specimen Preparation analogous to procedures used for light microscopy for transmission electron microscopy, specimens must be cut very thin specimens are chemically fixed and stained with electron dense materialuse TEM grids: use TEM gridsOther preparation methods: Other preparation methods shadowing coating specimen with a thin film of a heavy metal Negative staining potassium phosphotungstate uranyl acetateThe Scanning Electron Microscope: The Scanning Electron Microscope uses electrons reflected from the surface of a specimen to create image produces a 3-dimensional image of specimen’s surface featuresNewer Techniques in Microscopy: Newer Techniques in Microscopy confocal microscopy scanning probe microscopy -have extremely high resolution -can be used to observe individual atomsConfocal Microscopy: Confocal Microscopy confocal scanning laser microscope laser beam used to illuminate spots on specimen computer compiles images created from each point to generate a 3-dimensional imageScanning Probe Microscopy: Scanning Probe Microscopy atomic force microscope sharp probe moves over surface of specimen up and down movement of probe is detected and used to create image Study of surface structure of bacteriaImmunoelectron microscopy : Immunoelectron microscopy Allows identification several Ag Electron dense labelling of F c portion of specific Ab Anti-immunoglobulin reagent You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
electron microscope bablipradnya Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 49 Category: Entertainment License: All Rights Reserved Like it (0) Dislike it (0) Added: February 02, 2012 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Electron Microscope: Electron Microscope Dr. Pradnya GajbhiyeElectron Microscope: Electron Microscope • In 1932, invented by E. Ruska et al. • In 1986, Ruska received the Nobel PrizePowerPoint Presentation: Complex, costly Technically difficult to maintain Principle Closely parallel to optical micrroscopeElectron Microscope: Electron Microscope beams of electrons are used to produce images wavelength of electron beam is much shorter than light resulting in much higher resolutionPowerPoint Presentation: Best resolution obtained 3-5 A o i.e. 0.3-0.5 mµSpecimen Preparation: Specimen Preparation analogous to procedures used for light microscopy for transmission electron microscopy, specimens must be cut very thin specimens are chemically fixed and stained with electron dense materialuse TEM grids: use TEM gridsOther preparation methods: Other preparation methods shadowing coating specimen with a thin film of a heavy metal Negative staining potassium phosphotungstate uranyl acetateThe Scanning Electron Microscope: The Scanning Electron Microscope uses electrons reflected from the surface of a specimen to create image produces a 3-dimensional image of specimen’s surface featuresNewer Techniques in Microscopy: Newer Techniques in Microscopy confocal microscopy scanning probe microscopy -have extremely high resolution -can be used to observe individual atomsConfocal Microscopy: Confocal Microscopy confocal scanning laser microscope laser beam used to illuminate spots on specimen computer compiles images created from each point to generate a 3-dimensional imageScanning Probe Microscopy: Scanning Probe Microscopy atomic force microscope sharp probe moves over surface of specimen up and down movement of probe is detected and used to create image Study of surface structure of bacteriaImmunoelectron microscopy : Immunoelectron microscopy Allows identification several Ag Electron dense labelling of F c portion of specific Ab Anti-immunoglobulin reagent