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Edit Comment Close Premium member Presentation Transcript Nanotechnology: Environmental Impact : Nanotechnology: Environmental Impact Center for Biological and Environmental Nanotechnology : Center for Biological and Environmental Nanotechnology Science and Engineering at the WET / DRY interface of Nanotechnology MOTIVATION : Nano in Bio Medicine The unique optical, electronic, structural properties of the dry side when added to biomolecules will result in powerful new medical treatments and diagnoses. Bio in Nano Materials/Devices Manipulation and assembly of nanostructures from the dry side into materials and functional devices using machinery from the wet side will enable new technologies. Nano-Bio in our Environment Responsibility Risk Assessment / Risk Reduction MOTIVATION Natural Nanoconjugates (3A/1A) : Natural Nanoconjugates (3A/1A) 2) Aggregation (ppt) 3) Biotic Uptake Nanotechnology and the Environment : Nanotechnology and the Environment “A tool for sustainable development rather than an environmental liability” Enabling technologies to meet many needs water, wastewater hazardous waste resource recovery pollution prevention Environmental consequences of new technologies focus on transport, transformation, and fate of nanostructures proactive approach early in the process Water treatment plant Lessons from Earlier Technologies : Lessons from Earlier Technologies Past failures to consider environmental consequences early have been costly: semiconductor industry (metals, solvents) synthetic chemicals (PCB, DDT, Freon) applications of natural compounds (chlorine, asbestos) transportation, energy (air pollution, global warming, nuclear wastes) Small adjustments early in the trajectory of a technology have large consequences. Must introduce the environmental perspective early into the culture of emerging technologies (research and educational missions). Ozone hole Nanostructures: Environmental Impact? : Nanostructures: Environmental Impact? Particle-mediated transport size surface chemistry (hydrophobic) Potential for bio-assimilation direct consequences associated contaminants Nanoparticles in Aqueous Environments : Nanoparticles in Aqueous Environments Bio-uptake aggregation deposition naturally occurring particles organic compounds/ macromolecules/ contaminants nanoparticles transport 3A2 3A3 3A1 Separation Technologies : Assess capabilities of existing separation technologies Environmental quality control Nanomaterial production Conceive new separations processes Separation Technologies Need knowledge of fundamentals of transport and chemistry at the nanoscale. Water treatment facility Computational fluid dynamics simulation (Wiesner) Nanostructured Membranes for Separations : Nanostructured Membranes for Separations Tailor membranes for permeability, selectivity, reactivity, low fouling narrow pore size distribution high porosity high specific surface area nanostructured asymmetry Asymmetric membrane (Barron, Wiesner) Pore size distribution of alumoxane membrane (Barron, Wiesner) Two initial strategies metal-oxane derived membranes deposition-controlled template design Value Added by Center : Value Added by Center Environmental risk assessment Nanomanufacturing facility Integrated research program and expertise Computation (transport/removal of nanostructures) Materials chemistry (membrane design) Nano surface chemistry (adsorption/desorption) Biology (bio-uptake into cells) Why should we be concerned now? : Why should we be concerned now? Nanotechnology is a rapidly growing field of industry Predicted to be $700 billion market by 2008, Nanobusiness Alliance, November 2001 Preventative measures preferable Past industrial failures associated with biouptake.accumulation have had grave environmental consequences (i.e., DDT) More economical Biouptake of Nanoparticles : Biouptake of Nanoparticles Nanoparticles may enter cells via: Endocytosis Receptor activation for initiation Membrane penetration Generally occurs with very hydrophobic particles Transmembrane channels May be seen only with very small nanoparticles (< 5 nm) Biouptake and Adsorption : Biouptake and Adsorption Many types of molecules will adsorb to nanoparticles in complex aqueous environments Adsorbed molecules may dictate biological interactions, especially biouptake Biomolecules (i.e. proteins) Synthetic chemicals (i.e. pesticides) Factors Influencing Adsorption : Factors Influencing Adsorption Occurs to a greater degree on: Hydrophobic particles Charged Particles Amphiphilic or charged molecules most likely to adsorb Macromolecules generally adsorb most strongly Specific Protein Interactions with Nanoparticles Can Occur : Specific Protein Interactions with Nanoparticles Can Occur Example: Antibody interacting with C60 Provided by Jianpeng Ma Other examples:Semi- conductor binding peptides Metal-binding peptides Cellular Uptake of Nanoparticles : Cellular Uptake of Nanoparticles So far, most nanoparticles do not interact with cells unless their surface is bound to cell-interacting molecules Receptor-targeted Nanoparticles Non-targeted Nanoparticles More Studies are Needed to Understand Biouptake! : More Studies are Needed to Understand Biouptake! Systematic studies over a range of nanoparticles sizes and surface chemistries Evaluation of specific Nanoconjugates Nanoparticles bound to particular molecules that may play a role in biouptake Bioaccumulation? : Bioaccumulation? Accumulation of a substance within a species due to lack of degradation or excretion Most nanoparticles are not biodegradable If nanoparticles enter organisms low in the food web, they may be expected to accumulate in organisms higher in the food web Need to understand possible health effects of nanoparticle exposure! Nanoparticle Aggregation : Nanoparticle Aggregation In complex aqueous environments, many types of nanoparticles undergo aggregation Biological interactions with aggregated nanoparticles similar to bulk materials Intracellular aggregation may cause extensive damage and induce cell death May expect similar results to diseases caused by protein aggregation (i.e., sickle cell disease) Facilitated Transport of Toxins : Facilitated Transport of Toxins Adsorbed molecules will enter cells if nanoparticles do Substances normally excluded from cells may then enter Substance may be toxic to that organism or ones further up the food web Toxin Any evidence for this? : Any evidence for this? Many studies show facilitated transport of heavy metals, fertilizers, and pesticides into fish Chemicals adsorb to naturally occurring colloidal particles, resulting in tremendous increases in biouptake Nanoparticle-Induced Activation of the Immune System : Nanoparticle-Induced Activation of the Immune System In an organism, proteins will be the dominant adsorbate. All extracellular proteins involved. Proteins change their conformation during adsorption, and thus may change function. Complement Activation : Complement Activation Clustered IgGs or 1 IgM Nucleophilic groups on surface Complement protein reactions via Classical Pathway Complement protein reactions via Alternative Pathway •Generation of Anaphylactic Agents C3A & C5A •Generation of Leukocyte Receptor C3BC5B Formation of Membrane Attack Complex (MAC) Tissue Damage Potential Effects? : Potential Effects? Inhaled particles induce inflammation in respiratory tract, causing tissue damage. Example: Inhalation of silica particles in industrial workers causes “silicosis”. Ingested nanoparticles may cause liver damage. Ingested nanoparticles (i.e. for oral drug delivery) have been found to accumulate in the liver. Excessive immune/ inflammatory responses cause permanent liver damage. Possible Induction of Auto-Immune Disorders : Possible Induction of Auto-Immune Disorders Macrophage Phagocytoses Foreign Material, Binds C3BC5B Phagocytosis Lymphocyte Produces Antibodies Macrophage “Instructs” lymphocytes to generate antibodies against phagocytosed material Y Y Y Y Y Y Y Y Y Antibodies These antibodies may recognize proteins that were adsorbed to the nanoparticles. These were denatured proteins from the same animal. Antibodies may cross-react with “native” proteins, inducing Auto-Immunity. Auto-Immune Disorders are Linked to Small Particulates : Auto-Immune Disorders are Linked to Small Particulates Example: Wear debris is generated by orthopedic implants. Patients with such implants have a statistically significant rise in the incidence of auto-immune diseases. Example: Industrial workers who breathe particulate matter (i.e. silica dust) have a significantly higher risk of auto-immune disorders. The “Dark Side” of Nanotechnology? : The “Dark Side” of Nanotechnology? There has not been enough research done to know what the biological implications of NanoIndustry will be. There is evidence to suggest possible problems. As a scientific community, we should be pro-active in addressing the possible risks. You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.