NEONATAL JAUNDICE

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Presentation Transcript

Neonatal Jaundice : 

Neonatal Jaundice Nirmala Roberts Paediatric Nursing India

Facts & figures : 

Facts & figures Commonest abnormal physical finding during I week of life > 2/3 of NBBs develop clinical jaundice Visible form of bilirubinemia Adult sclera >2mg / dl Newborn skin >5 mg / dl Occurrence - 25 – 50% of term, 80% of preterm NBBBs Yellow discoloration – First evident on skin of face, naso-labial folds and tip of nose Masked by physiological plethora Assess by blanching Assess in good daylight – No yellow light, clothes or curtains

Clinical assessment of jaundice : 

Clinical assessment of jaundice With increase in jaundice – Cephalo-pedal progression of yellow discoloration of skin - Imp! – Screen all NBBs x BD in good day light

Bilirubin turnover : 

Bilirubin turnover

Sources of Bilirubin : 

Sources of Bilirubin Haem containing proteins RBC Hb – Major source. 1 gm Hb  34 mg of Bilirubin - Haem from ineffective erythropoiesis in bone marrow - Other haem containing proteins – myoglobin, cytochromes, catalase, peroxidase - Free haem

Bilirubin turnover in Newborn : 

Bilirubin turnover in Newborn Hb  Haem + Globin Other sources BILIRUBIN UCB binds to S albumin Dissociates from albumin UDPG - T Bil monoglucoronide + Bil Diglucoronide Water sol Bil Binds to cytoplasmic Ligandin (Y protein) Entero-hepatic circulation Excreted into bile canaliculi Enters Gut Excreted in stool Biliverdin + CO + Fe Bilirubin Reductase Haem oxygenase Beta Glucoronidase UCB Excretion Conjugation

Physiological handicaps causing increased Bilirubin turnover : 

Physiological handicaps causing increased Bilirubin turnover 1. Increased production of bilirubin Physiological Polycythemia Shorter life span of HbF (90 days) 1ml/kg (approx 1% ) of blood hemolyse everyday 0.15 gm/ kg of Hb released everyday 1 gm Hb yields 35 mg of bilirubin Hence, a 3 kg infant produces 15 mg bil/ day from Hb sources + 1 mg/kg from non Hb sources Thus, daily load to the liver – 20 mg bil 2. Defective uptake from plasma Non availability of albumin binding sites

Physiological handicaps… : 

Physiological handicaps… 3. Defective conjugation Transient deficiency of Y & Z acceptor proteins 4. Reduced hepatic clearance Reduced UDPG enzymes >ed unconjugated bil in early days of life in preterms 5. Enhanced enterohepatic circulation 100 – 200 mg of bil in gut as 1 mg/gm of meconium Reduced gut motility & poor evacuation Paucity of bacterial flora in gut of NBB Over activity of intestinal glucoronidase enzyme Conj Bil rapidly deconjugated and recirculated through blood to liver for reconjugation

Bilirubin load causing jaundice in Newborn : 

Bilirubin load causing jaundice in Newborn >ed RBC vol & <ed RBC survival >ed Bil monoglucoronide <ed Bil Diglucoronide UCB Production Transport Uptake Excretion Conjugation >ed Ineffective erythropoiesis & >ed Heam turnover Non availability of Albumin binding sites Defective conjugation <ed LIigandin Decreased excretion <ed gut motility Poor evacuation >>ed beta glucoronidase, <ed intestinal bacteria >ed BILIRUBIN load Defective uptake from plasma >ed Entero-hepatic circulation

Causes of jaundice : 

Causes of jaundice

Within 24 hours of birth : 

Within 24 hours of birth Hemolytic disease of newborn - due to feto-maternal group incompatibility (Rh, ABO, other minor blood group systems) Intra-uterine infections (TORCH) Deficiency of red cell enzymes – G6 PD, pyruvate kinase, heokinase, phospho-glucose isomerase, unstable Hbs Admn of drugs in excess – Vit K, salycilates, sulfisoxazole to mother Hereditary spherocytosis Criggler Najjar syndrome Lucey Driscoll syndrome Homozygous alpha thalassemia

Between 24 – 72 hours of age : 

Between 24 – 72 hours of age Physiological jaundice May be aggravated/ prolonged by – Immaturity, birth asphyxia, acidosis, hypothermia, hypoglycemia, drugs, cephalhematoma, hge’/ bruising, Polycythemia, high altitude, cretinism, breast feeding, infections Mild hemolytic states – Feto maternal blood gp incompatibility, spherocytosis, deficiency of cell enzymes

After 72 hrs of age ( and within first 2 weeks) : 

After 72 hrs of age ( and within first 2 weeks) Septicemia Neonatal hepatitis Extra hepatic biliary atresia Breast milk jaundice Metabolic diseases – Galactosemia, tyrosinemia, fructosemia, organic acidemia, cyctic fibrosis, alpha 1 anti trypsin deficiency Hypertrophic pyloric stenosis & intestinal obstruction

Drugs causing increase in bilirubin : 

Drugs causing increase in bilirubin

Risk factors for jaundice : 

Risk factors for jaundice J - jaundice within first 24 hrs of life A - a sibling who was jaundiced as neonate U - unrecognized hemolysis N – non-optimal sucking/nursing D - deficiency of G6PD I - infection C – cephalhematoma /bruising E - East Asian/North Indian

Common causes in India : 

Common causes in India Physiological Blood group incompatibility Intrauterine and postnatal infections G-6PD deficiency Bruising and cephalhematoma Breast milk jaundice

Jaundice – Physiological & Pathological : 

Jaundice – Physiological & Pathological

Physiological jaundice : 

Physiological jaundice Appears between 30 – 72 hrs of age in term babies (earlier in preterms) Maximum intensity by 4th-5th day in term & 7th day in preterm Serum level less than 15 mg / dl Disappears by 10 days of life (reaches 15 days in preterms) Disappears without any treatment Note: Baby should, however, be watched for worsening jaundice Alert in preterms…danger of brain damage!

Pathological jaundice : 

Pathological jaundice In 5% of newborns Appears within 24 hours of age Increase of bilirubin > 5 mg / dl / day Serum bilirubin > 15 mg / dl Jaundice persisting after 14 days Stool clay / white colored and urine staining clothes yellow Direct bilirubin> 2 mg / dl

Dangers of hyperbilirubinemia : 

Dangers of hyperbilirubinemia Unconjugated hyperbilirubinemia  Bil encephalopathy/ Kernicterus C/F – Refusal of feeds, shrill cry, setting sun sign, convulsions, retrocollis and opisthotonus - Sluggish Moro’s response, lethsrgy, poor feeding - Preterms – Non specific. Die due to apneic attacks - Infancy – Athetoid cerebral palsy, choreo-athetosis, brownish staining of teeth, dental dysplasia, deafness, paralysis of upward gaze, intellectual retardation & learning disabilities

Pathogenesis of kernicterus : 

Pathogenesis of kernicterus Related to – Unconjugated bilirubin levels Gestational maturity of infant Integrity of blood-brain barrier Biochemical determinants – Bilirubin protein ratio – 3.5 or more HBABA (hydroxybenzene azo benzoic acid) dye binding capacity - < 50% Salicylate saturation index – 8 or moreRBC biuding of bilirubin - >4 mg%

Kernicterus : 

Kernicterus

Assessment : 

Assessment

Approach to a jaundiced baby : 

Approach to a jaundiced baby Ask 4 questions - What is the birth weight? What is the gestation? What is the postnatal age in hours? Is the jaundice physiological or pathological? If jaundice is physiological and baby is well – only observe If deep jaundice – Assess for bil toxicity (kernicterus)

Indication for lab investigations (In high risk infants) : 

Indication for lab investigations (In high risk infants) H/O Jaundice, Exchange Blood transfusion / Kernicterus in previous sibling Mother – O group or Rh –ve Jaundice – Within 24 hrs or after 72 hrs Trunk distinctly yellow stained Sick jaundiced baby Jaundice persisting > 2 weeks Yellow coloured urine or clay coloured stools

Workup : 

Workup Maternal & perinatal history Physical examination Laboratory tests (must in all) Total & direct bilirubin (Total : 0.3 to 1.9 mg % , Direct : 0 to 0.3 mg %) Bil: protein ratio (< 3.4) Blood group and Rh for mother and baby Blood - Hematocrit, retic count and peripheral smear, Sepsis screen, Carboxy Hb Liver function - Vanden Bergh test Thyroid function TORCH titers, liver scan when conjugated hyperbilirubinemia Tests for – Pulmonary excretion rate of CO, OR End tidal CO (Etco) breath level

Assessment of severity of jaundice : 

Assessment of severity of jaundice Assess in natural daylight for cephalo-pedal progression (Unreliable after phototherapy) Use of ‘Icterometer’ Transcutaneous bilimeter Bilimeter with microcentrifuge

Management : 

Management

Management of jaundice : 

Management of jaundice A medical emergency Aim – Keep S bilirubin at a safe level and prevent bil toxicity Prevent brain damage Methods – Preventive and supportive measures Reduction of bilirubin levels – Phototherapy, Exchange transfusion (-most effective and reliable method), drugs

Preventive and supportive measures : 

Preventive and supportive measures Prevention of Rh isoimmunization Withhold drugs that aggravate jaundice or block bilirubin binding sites in albumin Prevent perinatal distress factors Avoid phenolic detergents in nursery

Slide 31: 

Adequate feeding and hydration Aspiration of cephalhematoma (bil > 18 mg%) Treatment of sepsis and hepatitis Phenobarbitone Clofibrate – Enhances glucoronyl transferase

Measures to reduce Serum Bilirubin : 

Measures to reduce Serum Bilirubin Phototherapy Drugs blocking entero-hepatic circulation of bilirubin Exchange transfusion Sunlight –NO!

Maisel’s chart : 

Maisel’s chart Used for deciding on treatment of pathological jaundice. If any of the following present, treat as in next higher bilirubin category. Perinatal asphyxia Respiratory distress Metabolic acidosis Hypothermia Low serum protein Birth weight <1500 g Signs of clinical or CNS deterioration

Planning on intervention Maisel’s chart : 

Planning on intervention Maisel’s chart

Measures to reduce S Bilirubin - Phototherapy : 

Measures to reduce S Bilirubin - Phototherapy Safe and effective Bil absorbs light maximally at 420 – 460 nm Causes photo-oxidation & photo-isomerization of bilirubin Enhances hepatic exertion of unconjugated bil into the intestinal lumen Single & double surface system

Phototherapy equipment : 

Phototherapy equipment White light tubes 6-8*/ 4 blue light tubes Cradle or incubator Eye shades *May use 150 W halogen bulb

Babies under phototherapy : 

Babies under phototherapy Baby under conventional phototherapy Baby under triple unit intense phototherapy

Phototherapy : 

Phototherapy Safe and effective Bil absorbs light maximally at 420 – 460 nm Causes photo-oxidation & photo-isomerization of bilirubin Enhances hepatic exertion of unconj bil into the intestinal lumen Single & double surface system 6 - 8 daylight tubes or 4 blue/ white tubes mounted on a stand with grounded electrical outlets. Change tubes every 1000 hours or after 3 months of use Alternate - 150 watt halogen bulb (life 1000 hours) OR Blue CFL lamps - change every 3000 h.. Maintain flux at 6-8 mw/cm2/nm with help of fluxmeter. Have Plexiglas shield to cover the tube lights. Nurse in cradle or incubator

Phototherapy : 

Phototherapy Perform hand wash Place baby naked 45 cm away from the tube lights in a crib or incubator Fix eye shades - to prevent damage to the retina. Also cover gonads Keep baby at least 45 cm from lights, if using closer monitor temperature of baby Start phototherapy Frequent extra breast feeding every 2 hourly Turn baby every two hours or after each feed (in single surface) Monitor – Temp - 2 to 4 hourly, wt -daily, urine frequency, bilirubin - 12 hrly More frequent breastfeeding or 10-20% extra fluid is provided. Discontinue phototherapy - if two serum bilirubin values are < 10 mg/dl. Measure rebound bilirubin 6-8 hours after stopping phototherapy.

Slide 40: 

Turn baby every two hours or after each feed (in single surface) Monitor temperature of the baby x 2 – 4 hrly.. Monitor daily - Urine frequency and body wt More frequent breastfeeding or 10-20% extra fluid is provided. Monitor Serum bilirubin at least every 12 hours Discontinue phototherapy - if two serum bilirubin values are < 10 mg/dl. Measure rebound bilirubin 6-8 hours after stopping phototherapy. Remember - Baby appears bleached when under phototherapy. Hence clinical assessment of jaundice not reliable. Monitor S bilirubin.

Side effects of phototherapy : 

Side effects of phototherapy Increased insensible water loss Loose stools Skin rash Bronze baby syndrome Hyperthermia Upsets maternal baby interaction May result in hypocalcemia

Drugs blocking entero-hepatic circulation of bilirubin : 

Drugs blocking entero-hepatic circulation of bilirubin

Exchange transfusion : 

Exchange transfusion Most effective and reliable method to reduce serum bilirubin. Anticipation and early referral to a higher centre is important Indications – Cord Hb 10 gm% or less Cord bilirubin 5 mg% or more Unconj S bil 10 mg% within 24 hrs or 15 mg% within 48 hrs or rate of rise of 0.5 mg% per hr

Choice of blood for exchangeblood transfusion : 

Choice of blood for exchangeblood transfusion ABO incompatibility Use O cells of same Rh type, ideal - O cells suspended in AB plasma. Rh isoimmunization In emergency 0 -ve blood. Ideal 0 -ve suspended in AB plasma or baby's blood group but Rh –ve blood also Other situations Baby's blood group

Indications for Exchange Transfusion : 

Indications for Exchange Transfusion Hyperbilirubinaemia (Rhesus/ABO incompatibility) to prevent kernicterus Anaemia/ Hydrops Polycythemia ie. partial exchange Rare conditions Hyperkalaemia Drug toxicity/overdose Disseminated intravascular coagulation Maternal AntibodiesEY

Slide 47: 

Use circumstraint to immobilize

The exchange equipment : 

The exchange equipment The volume of blood required - . 1. Single blood volume exchange for anaemia: Term infant – 80 -100mls /kg Preterm infant – 100mls/kg Volume exchanged (ml) = Wt (kg) x Blood Volume x (Hb desired – Hb initial) 2. Double volume exchange for jaundice: (Approx 2 x 85 mls/kg) - Term infant – 200 -250 mls/kg or 80 – 85 mls/kg x 2/3 - Preterm infant – 300 mls/kg or 100 – 120 mls/kg x 2/3

Procedure : 

Procedure 'PUSH-PULL METHOD’ via the umbilical vein. - Serial withdrawal and injection of aliquots (5-20 mls) – 1 mt for each cycle. Takes 60 – 120 minutes. - Used when arterial arterial access is a problem. ‘ISOVOLUMETRIC METHOD’ - slow removal of aliquots (10mls usually) from an artery (central or peripheral) and simultaneous infusion into a vein (central or peripheral). - preferred method - Does not cause wide fluctuations of blood volume and pressure. - Takes 60 – 90 minutes

Exchange transfusion : 

Exchange transfusion

First out specimen tested for - : 

First out specimen tested for - Haemoglobin, film, PCV Group, Rhesus, Direct Coomb's test PGL Urea and electrolytes, calcium, SBR, total and conjugated Blood gas Coagulations profile Newborn screening test Hold samples for other tests as indicated, eg. G6PD deficiency, Viral infection, hereditary spherocytosis, metabolic studies.

Set up for Push Pull method : 

Set up for Push Pull method UAC tray and catheters Sterile drapes Blood administration set 3-way taps X 2 (white blood in, red for blood out to waste) Exchange transfusion recording sheet Blood warmer with appropriate coil Extension tubing, long - must be wide bore Drainage connecting tube 5ml, 10ml, and 20ml syringes depending on size of aliquots to be used Waste bag Calcium gluconate 10% ampoules Heparin ampoules1000 IU/ml or heparinised NaCl 0.9% 50 ml syringe