logging in or signing up tissue culture alokkumar_89 Download Post to : URL : Related Presentations : Let's Connect Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 1047 Category: Education License: All Rights Reserved Like it (0) Dislike it (0) Added: May 23, 2011 This Presentation is Public Favorites: 2 Presentation Description Plant Tissue Culture Comments Posting comment... Premium member Presentation Transcript Plant Tissue Culture: 1 Plant Tissue Culture presented by alok kr vishwakarma (firstname.lastname@example.org) katiyar bipin sarvesh ( email@example.com ) Dept of pharmaceutics psit,kanpurHistory: 2 History 1965 French Botanist discovers Micropropagation Virus-free orchid plant Beginning of Tissue CultureWhat is Tissue Culture?: 3 What is Tissue Culture? Asexual Propagation aka Micropropagation Defined as: Production of new plantlets from tissue of an actively growing plantWhy use tissue culture?: 4 Why use tissue culture? Species with any of the following: Regeneration problems Poor germination Desirable traits Disease-resistance High productivity Gender of commercial importance Male- Asparagus Female- PapayaWhere is it used?: 5 Where is it used? Commercial Production Chrysanthemum Gladiolus Etc. Crop production Sugar cane Tapioca PotatoesWhat is required?: 6 What is required? Very sanitary conditions Usually a lab Sterile aseptic culture or MEDIA containing: Nutrients Sugars Vitamins Hormones Test tube Plant tissue: shoot tip, leaf section, meristem, node, etc.The Procedure : 7 The Procedure Prepare the media Sterilize everything including: Media Tools Supplies Equipment Work area Place media in test tube Remove tissue from plantProcedure continued: 8 Procedure continued Sanitize tissue sample Place tissue sample on media in test tube Cap or seal test tube Place in warm, well-lighted area Plantlets should begin to form in 4-6 weeksTissue Culture Process: 9 Tissue Culture Process Start Tissue Removed Media Callus PlantletAdvantages Disadvantages: 10 Advantages Disadvantages Small part of plant is required Produce many plantlets in small areas Disease-free plants Pest-resistant Maintaining a sterile environment Expensive lab facilities Poor photosynthesis Water lossGuided Practice: 11 Guided Practice Group Activity: Put the steps for tissue culture in order with your group.Independent Practice: 12 Independent Practice What is another name for tissue culture? Define tissue culture in your own words. List one advantage and one disadvantage of tissue culture. What four materials are required for the tissue culture process?Slide 13: 13 Thank u You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.