logging in or signing up Shigella abhijitgg25 Download Post to : URL : Related Presentations : Let's Connect Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 135 Category: Entertainment License: All Rights Reserved Like it (0) Dislike it (0) Added: August 11, 2013 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Book Antiqua: 1 Shigella Dr.Gogoi;University of Fiji Wingdings: 2 Shigella Belong to Enterobacteriaceae Non motile , non capsulated NLF on MAC Deoxycholate citrate agar (DCA) selective media (pale color colonies) Gram negative bacilli Times New Roman: 3 Shigella species Mannitol Non Fermentation Fermentation S dysentriae -12 S flexneri- 6 S boydii - 18 S sonnei (Late lactose fermenter) Arial Black: 4 Epidemiology and Clinical Syndromes Major cause of bacillary dysentery Outbreaks in daycare centers, nurseries, institutions Estimated 15% of pediatric diarrhea in U.S. Leading cause of infant diarrhea & mortality in developing countries Epidemics of S. dysenteriae type 1 have occurred in Africa and Central America with case fatality rates of 5-15%. Common in P ediatric age group (1-10 yrs) Developed world – S sonnei , developing world – S flexneri Hardcover: 5 Epidemiology and Clinical Syndromes Shigellosis = Generic term for disease Low infectious dose (10 2 -10 4 CFU) Humans are only reservoir Transmission by fecal-oral route Incubation period = 1-3 days Watery diarrhea with fever; changing to dysentery Bacillary Dysentry Passing blood & mucus in the stool Bitmap Image: 6 Infection cycle of Shigella Shiga toxin exacerbate inflammation by causing ishchemia & hemorrhage Ulcers Rarely lead to bacteremia Organisms adhere to colonic lymphoid follicles Undergo parasite directed phagocytosis Resist intracelluar killing Move directly from cell to cell SMALL GUT LARGE GUT STOMACH Shigella: 7 Pathogenesis Two-stage disease Early stage Second stage Fever attributed to neurotoxic activity of toxin D ysentery due to a dherence and tissue invasion of large intestine ( c ytotoxic activity of Shiga toxin ) Watery diarrhea attributed to the enterotoxi n activity of Shiga toxin in the small intestine Enterotoxic , neurotoxic and cytotoxic Shiga toxin Similar to Shiga-like toxin of E nterohemorrhagic E. coli (EHEC) Shigella: 8 Virulence factors Invasiveness Attachment (adherence) and internalization with complex genetic control Large multi-gene virulence plasmid regulated by multiple chromosomal genes Intracellular survival & multiplication Exotoxin (Shiga toxin) Complications : Hemolytic uremic syndrome and reiters syndrome Shigella species: 9 Clinical manifestation Fever Abdominal cramping & tenderness Diarrhea / Dysentry Tenesmus (straining at stools Sigmoidoscopy: ulceration PowerPoint Presentation: 8/11/2013 10 Lab Diagnosis of Dysentry Specimens Stool, rectal swab from an ulcer ( buffered glycerol saline - transport medium) Culture MAC NLF colonies AST Report & Interpretations TSI agar K - / A - DCA NLF PowerPoint Presentation: 11 Shigella Dysentry- Differentiated from Dysentry like diseases Entero invasive E coli Campylobacter jejuni Entamoeba histolytica Balantidium coli PowerPoint Presentation: 12 Treatment For adults No vaccines Rehydration if necessary For children Co-trimoxazole or ampicillin + rehydration Norfloxacin or ciprofloxacin Water supply Sewage maintenance Human carriers identified & treated Breast feeding than formulas Prevention PowerPoint Presentation: 13 Summary Bacillary Dysentry Clinical manifestation Species of Shigella Pathogenesis Virulence factors Source and spread Laboratory diagnosis Antibiotics used Preventive measures You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.