Anti-inflammatory Activity of Ficus Bengalensis By Sharad Darade

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Department of Pharmacology SSDJ College of pharmacy, Chandwad. RESEARCH STUDENT: Sharad B.Darade T. Y. B. Pharm RESEARCH GUIDE : Mr. Manoj S.Mahajan Anti inflammatory activity of Ficus bengalnensis

CONTENTS : 

2 CONTENTS Introduction and reviews of literature 2. Plant profile 3. Objective and plan of work 4. Mat erials and methods 5. Results 6. Discussion 7. Conclusion 8. Future scope 9. Reference 10. Publications

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3 INFLAMMATION Inflammation or phlogosis is the complex pathophysiological response of vascularised tissue to injury. INTRODUCTION AND REVIEW OF LITERATURE TYPES OF INFLAMMATION Acute Inflammation Chronic inflammation 1. Heat 2. Redness3. Swelling 4. Pain5. Loss of function 5 CLASSICAL SIGNS OF INFLAMMATION

Phases Of Inflammation : 

4 Phases Of Inflammation 1.Acute vascular response 2.Acute cellular response 3.Chronic cellular response and Resolution Responses during inflammation 1.Tissue macrophage 2.Neutrophil invasion 3.Second macrophage 4.Granulocytes & monocytes

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5 A substance that causes one of the component events in inflammation through a specific receptors is called a mediator of inflammation. Acute Inflammation: Eicosanoids, Histamine, Serotonin Chronic Inflammation: Cytokine, Interleukin Mediators Of Inflammation

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6 Inflammation & Disease Development

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7 Treatment of inflammation Steroidal Anti-inflammatory Drugs Non-steroidal Anti-inflammatory Drugs

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8 Pharmacological models of inflammation Rat paw edema Vascular permeability Oxazolone induced ear edema in mice Pleurisy test Granuloma pouch technique Urate induced synovitis

Plant Profile : 

9 Plant Profile Scientific classification Kingdom: Plantae Division: Magnoliophyta Class: Magnoliopsida Order: Urticales Family: Moraceae Genus: Ficus Species: F. bengalensis

Objective & plan of work : 

10 Objective & plan of work OBJECTIVE The present study was undertaken to evaluate the anti-inflammatory activity of Ficus bengalnensis in well established animal models PLAN OF WORK To study the effect of Ficus bengalnensis on carrageenan induced paw edema To study the effect of Ficus bengalnensis on formalin induced rat paw edema

Materials & methods : 

11 Materials & methods Animals 50 male Albino rats (220 -240gm) obtained from National toxicology Centre, Pune. The Institutional Animal Ethical Committee(IAEC)approved the protocol of this study. Plant Material The leaves of Ficus bengalnensis collected from local region and extracted with methanol using soxhelt apparatus . Drugs and Chemicals Aspirin, carrageenan, gum acacia, sodium chloride, formalin etc.

Experimental grouping : 

12 Experimental grouping Carrageenan induced hind paw edema: Control: Standard: FB 10 FB 30 FB 100 Formalin induced rat paw edema: Control Standard FB 10 FB 30 FB 100

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13 Principle Calibration Measurement Plethysmometer (Ugo basile 7140, S.N. 396)

Results : 

14 Results Effect of MFB on carrageenan induced rat paws edema * P<0.05 as compared with Control Effect of MFB on formalin induced rat paws edema * P<0.05 as compared with Control

Discussion : 

15 Discussion Ficus bengalnensis Chemical constituents of F.bengalnensis Anti inflammatory potential of F.bengalnensis

Conclusion & Future Scope : 

16 Conclusion & Future Scope Further research is required to find out the exact mechanism of action of the methanol root extract of Ficus bengalensis Results showed significant effect of treatment on % inhibition of paw volume. This effect was dose dependant. From this, it was concluded that the natural medicine Ficus bengalensis possesses anti-inflammatory activity and was the good alternative for NSAIDs.

Bibliography : 

17 Bibliography Arend WP. (1993). Interleukin-1 receptor antagonist. Adv. Immunol., 54: 167-227. Arora RB, Kapoor V, Gupta SK, Sharma RC. (1971). Isolation of a crystalline steroidal compound from Commiphora mukul & its anti-inflammatory activity. Indian J Exp Biol 9:403-405    Arora RB, Basu N, Kapoor V, Jain AP. (1971).Anti-inflammatory studies on Curcuma- longa (turmeric). Ind J Med Res Aug; 59(8):1289-95). Bhoola KD, Figueroa CD, and Worthy K. (1992) Bioregulation of kinins: kallikreins, kininogens, and kininases. Pharmacol.Rev., 44: 1-80. Braquet P, Touqui L, Shen TY, Vargaftig BB. (1987). Perspectives in platelet activating factor research. Pharmacol Rev. 39: 97-145. Celsus AC. De medicina. Self published; A.D. 25. Chatterji TK, Sardar M, Bal Subramanian T, & Lal SM. evalution of anti-inflammatory analgesic, antipyretic activity of aqueous extract of medicinal plant scoparia dulcis, edited & published by Dr.A.patani , Vol.45(5) page no.362-369 Green GA. (2001). Understanding NSAIDS: from aspirin to COX-2. Clin Cornerstone 3(5):50-59. Guyton AC, and Hall JE. (2001) Resistance of the body to infection: 1.Leukocytes, Granulocytes, the Monocyte-Macrophage system and Inflammation. In, Textbook of Medical Physiology. 10th ed. Gopsons Papers Ltd., Noida, pp. 397-399.

PUBLICATION & PRESENTATION : 

18 PUBLICATION & PRESENTATION A paper presented at 41st annual conference of Indian Pharmacological society, New Delhi (December 18th-20th 2008) Subject code : Immune & Inflammatory system Presentation: Poster Analgesic and Anti-inflammatory activity of Ficus bengalnensis Darade S , Alai M , Patel E, Gadiya P, Gaikwad S, , Gulecha V Dept. of Pharmacology SSDJ College of Pharmacy, Neminagar, Chandwad, Nashik

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THANK YOU !!!