Slide 1: Department of Pharmacology
SSDJ College of pharmacy, Chandwad. RESEARCH STUDENT: Sharad B.Darade
T. Y. B. Pharm RESEARCH GUIDE : Mr. Manoj S.Mahajan Anti inflammatory activity of
Ficus bengalnensis CONTENTS : 2 CONTENTS Introduction and reviews of literature
2. Plant profile
3. Objective and plan of work
4. Mat erials and methods
8. Future scope
10. Publications Slide 3: 3 INFLAMMATION Inflammation or phlogosis is the complex pathophysiological response of vascularised tissue to injury. INTRODUCTION AND REVIEW OF LITERATURE TYPES OF INFLAMMATION
Chronic inflammation 1. Heat 2. Redness3. Swelling 4. Pain5. Loss of function 5 CLASSICAL SIGNS OF INFLAMMATION Phases Of Inflammation : 4 Phases Of Inflammation 1.Acute vascular response
2.Acute cellular response
3.Chronic cellular response and Resolution
Responses during inflammation
4.Granulocytes & monocytes Slide 5: 5 A substance that causes one of the component events in inflammation through a specific receptors is called a mediator of inflammation.
Acute Inflammation: Eicosanoids, Histamine, Serotonin
Chronic Inflammation: Cytokine, Interleukin Mediators Of Inflammation Slide 6: 6 Inflammation & Disease Development Slide 7: 7 Treatment of inflammation Steroidal Anti-inflammatory Drugs
Non-steroidal Anti-inflammatory Drugs Slide 8: 8 Pharmacological models of inflammation Rat paw edema
Oxazolone induced ear edema in mice
Granuloma pouch technique
Urate induced synovitis Plant Profile : 9 Plant Profile Scientific classification
Species: F. bengalensis Objective & plan of work : 10 Objective & plan of work OBJECTIVE
The present study was undertaken to evaluate the anti-inflammatory activity of Ficus bengalnensis in well established animal models PLAN OF WORK
To study the effect of Ficus bengalnensis on carrageenan induced paw edema
To study the effect of Ficus bengalnensis on formalin induced rat paw edema Materials & methods : 11 Materials & methods Animals
50 male Albino rats (220 -240gm) obtained from National toxicology Centre, Pune. The Institutional Animal Ethical Committee(IAEC)approved the protocol of this study.
The leaves of Ficus bengalnensis collected from local region and extracted with methanol using soxhelt apparatus . Drugs and Chemicals Aspirin, carrageenan, gum acacia, sodium chloride, formalin etc. Experimental grouping : 12 Experimental grouping Carrageenan induced hind paw edema:
FB 100 Formalin induced rat paw edema:
FB 100 Slide 13: 13 Principle
Measurement Plethysmometer (Ugo basile
7140, S.N. 396) Results : 14 Results Effect of MFB on carrageenan induced rat paws edema
* P<0.05 as compared with Control Effect of MFB on formalin induced rat paws edema
* P<0.05 as compared with Control Discussion : 15 Discussion Ficus bengalnensis
Chemical constituents of F.bengalnensis
Anti inflammatory potential of F.bengalnensis Conclusion & Future Scope : 16 Conclusion & Future Scope Further research is required to find out the exact mechanism of action of the methanol root extract of Ficus bengalensis Results showed significant effect of treatment on % inhibition of paw volume. This effect was dose dependant. From this, it was concluded that the natural medicine Ficus bengalensis possesses anti-inflammatory activity and was the good alternative for NSAIDs. Bibliography : 17 Bibliography Arend WP. (1993). Interleukin-1 receptor antagonist. Adv. Immunol., 54: 167-227.
Arora RB, Kapoor V, Gupta SK, Sharma RC. (1971). Isolation of a crystalline steroidal compound from Commiphora mukul & its anti-inflammatory activity. Indian J Exp Biol 9:403-405
Arora RB, Basu N, Kapoor V, Jain AP. (1971).Anti-inflammatory studies on Curcuma- longa (turmeric). Ind J Med Res Aug; 59(8):1289-95).
Bhoola KD, Figueroa CD, and Worthy K. (1992) Bioregulation of kinins: kallikreins, kininogens, and kininases. Pharmacol.Rev., 44: 1-80.
Braquet P, Touqui L, Shen TY, Vargaftig BB. (1987). Perspectives in platelet activating factor research. Pharmacol Rev. 39: 97-145.
Celsus AC. De medicina. Self published; A.D. 25.
Chatterji TK, Sardar M, Bal Subramanian T, & Lal SM. evalution of anti-inflammatory analgesic, antipyretic activity of aqueous extract of medicinal plant scoparia dulcis, edited & published by Dr.A.patani , Vol.45(5) page no.362-369
Green GA. (2001). Understanding NSAIDS: from aspirin to COX-2. Clin Cornerstone 3(5):50-59.
Guyton AC, and Hall JE. (2001) Resistance of the body to infection: 1.Leukocytes, Granulocytes, the Monocyte-Macrophage system and Inflammation. In, Textbook of Medical Physiology. 10th ed. Gopsons Papers Ltd., Noida, pp. 397-399. PUBLICATION & PRESENTATION : 18 PUBLICATION & PRESENTATION A paper presented at 41st annual conference of
Indian Pharmacological society, New Delhi (December 18th-20th 2008)
Subject code : Immune & Inflammatory system
Analgesic and Anti-inflammatory activity of Ficus bengalnensis
Darade S , Alai M , Patel E, Gadiya P, Gaikwad S, , Gulecha V
Dept. of Pharmacology
SSDJ College of Pharmacy, Neminagar, Chandwad, Nashik Slide 19: THANK YOU !!!