logging in or signing up Thin layer chromatography (College) aSGuest34196 Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 7962 Category: Education License: All Rights Reserved Like it (9) Dislike it (0) Added: December 16, 2009 This Presentation is Public Favorites: 9 Presentation Description No description available. Comments Posting comment... By: Shyam03 (29 month(s) ago) HI...could you please send me the ppt about TLC to 'email@example.com' Thank you. Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript Slide 1: Thin Layer chromatography By Abhisek Bhunia Topic include : Topic include Introduction : In 1944, Consden,Gorden & Martin used filter papers for separating the Amino acids. In 1950, Kirchner identified terpenes on filter paper. In 1958,STAHL develop standard equipment for analysing by THIN LAYER CHROMATOGRAPHY . Introduction Principle : The Principle is based on ADSORPTION Chromatography The component with more affinity towards the stationary phase travels slower. The component with lesser affinity towards the stationary phase travels faster. Principle Reverse phase chromatography : In this the stationary phase is Non-polar & mobile phase is polar & it is widely used in pharmaceutical analysis. Polar compound get eluted first Non-polar compounds are retained for long time Comparison of Normal phase & Reverse phase : Reverse phase chromatography Stationary phase : Stationary phase Mobile phase : Nature of the substance to be separated i e weather it is polar or non-polar. Mode of Chromatography. Nature of Stationary phase. Mode Separation i e Analytical or Preparative. Examples: 1) Petroliam ether 2) Cyclohexane 3) Acetone 4) Toluene 5) Ethyl acetate 6) Benzene 7) Alcohols 8) Water 9) Chloroform 10) Pyridine Mobile phase Glass plates : Three types : they are , Full plate : 20cm × 20 cm. Half plate : 20cm × 10 cm. Quarter plate : 20cm × 5 cm. Microscopic slides can also be used for Monitoring the progress of a chemical reaction. Glass plates Preparation & activation of plates : The T L C plates can be prepared by following techniques : Pouring. Dipping. Spraying. Spreading. Activation : It is nothing but removing of water/ moisture & other adsorbed substance from the surface of any adsorbent by heating. Preparation & activation of plates Application of sample : Application of sample Development tank : The development tank should be lined Inside with filter paper moistened with mobile phase to saturate the atmosphere & also prevent the “ EDGE EFFECT ” . Development tank Development technique : Different development techniques are : One dimensional development. Two dimensional development. Horizontal development. Multiple development. Development technique Detecting agents : Detecting agents are two types. they are, Non-Specific method 1) Iodine chamber method. 2) Sulphuric acid spray method. 3) UV chamber for fluorescent compounds. 4) Using fluorescent stationary phase. (B) Specific method 1) Ferric chloride. 2) Ninhydrine in acetone. 3) Dregendroff reagent. 4) 3,5 – Dinitro benzoic acid. 5) 2,4 - Dinitro phenyl hydrazine. Detecting agents detection : The Rf value is calculated for identification "Rf value is the ratio of distance travelled by The solute to the distance travelled by the solvent front” Distance travelled by solute Rf = Distance travelled by solvent front detection Development of t l c : Development of t l c application : Separation of mixture of drug of chemical,biological,plant origin. Separation of Carbohydrates, vitamin, antibiotics, proteins, etc. Identification of drug. Ex :Amoxicillin, Levodopa Detection of foreign substances. To detect the decomposition products of drug. application Comparison of tlc & paper chromatography : Comparison of tlc & paper chromatography Comparison of tlc & hplc : Comparison of tlc & hplc Reference : E Stahl. Thin-layer Chromatography. A Laboratory Handbook: Springer – Verlag Berlin . Heidelberg ,New York 1969:second edition 1990. G Chatwal,S anand.Ihstrumental Methods of Chemical analysis: Himalaya publishing house,Delhi:Edition June 1995. Dr.S. Ravi Sankar.Text book of pharmaceutical analysis:Rx Publication,Tiruneveli:Edition 1997,1999,2001. H.F.Waltor,J.Reyes:Modern Chemical analysis and instrumentation. International Medical Book distrubutor,Mumbai. Thin layer chromatography is available from www.encyclopidia.org. Thin layer chromatography is available from www.chem.vt.edu Thin layer chromatography is available from www.orgchem.com Thin layer chromatography is available from www.wellesly.eud Reference Slide 20: THANK YOU You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.