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Premium member Presentation Transcript Laboratory animals: practical training in Experimental Techniques : Laboratory animals : practical training in Experimental Techniques D.Eswar Tony Chalapathi institute of pharmaceutical sciences GunturLecture outline: Lecture outline Need of animals for research and teaching Handling of animals Routes of administration Collection of blood samples by different routesObjectives: Objectives To know and identify the commonly used laboratory animals in experimentation To understand the principles in Handling and restraining Administration of substances Specimen collection To determine the sexes of laboratory animals To handle and restrain correctly the commonly used laboratory animals To familiarize with the techniques in substance administration and specimen collectionPowerPoint Presentation: Using Animals in Research and EducationQuick Questions:: Quick Questions: Why do we need to use animals for research and teaching? What have people learned from animal research? Are the animals used in research & education protected and taken care of?Why Do We Need To Use Animals for Research & Teaching?: Why Do We Need To Use Animals for Research & Teaching? The functions of cells and organs are basically the same in animals and humans. What we learn from animals is useful in human and animal medicine. Animal cells function in many of the same ways as human cells. Biologically, humans are in the Animal Kingdom. An animal cellWhy Do We Need To Use Animals for Research & Teaching?: Why Do We Need To Use Animals for Research & Teaching? Animal are used to: Understand how diseases affect living tissue Develop and test treatments — including treatments for animals Train future scientists and health-care professionalsCan Results from Animal Studies Really Be Applied to Humans?: Can Results from Animal Studies Really Be Applied to Humans? They CAN and ARE . Virtually all drugs, devices and medical procedures have been developed with some animal research. This dog, Kodi, underwent hip replacement surgery twice. Hip replacement surgical techniques were tested first on animals and now help both animals and people.Animal Use in Biomedical Research: Animal Use in Biomedical Research Infant Mortality Studies in sheep led to use of steroids in treatment of respiratory distress syndrome, a major cause of death in premature infants. Advances in understanding and treatment of sudden infant death syndrome (SIDS) came from studies in rats, mice, dogs, and sheep.Animal Use in Biomedical Research : Animal Use in Biomedical Research Cystic Fibrosis A major killer of young adults. Mouse models led to understanding the biochemical processes involved in this disorder. Genetic therapies on the horizon are an extension of work in mice.Animal Use in Biomedical Research: Animal Use in Biomedical Research High Blood Pressure (HBP) Goldblatt linked HBP to kidneys in rats, cats, and dogs. This research led to treatments for high blood pressure. Cushing linked HBP to brains in dogs. This research led to understanding the nervous system’s influence on blood pressure and development of drugs to treat it.Animal Use in Biomedical Research: Animal Use in Biomedical Research Obesity Major risk factor for diabetes mellitus, high blood pressure, heart attack, stroke and certain cancers Epidemic in the United States: 64% of adults are overweight and 25% are obese Mouse models and Zucker obese rats are shedding new light on causes of overeating, importance of leptin receptors, and ways that obesity leads to disease.PowerPoint Presentation: AIDS Current anti-AIDS treatment developed in animals have greatly extended life expectancy and quality of life for AIDS victims. AIDS vaccines are being developed in monkeys.Animal Use in Biomedical Research: Animal Use in Biomedical Research Stroke Stroke kills over 150,000 people in the U.S. each year and cause major disabilities that can include paralysis, inability to speak, loss of vision and loss of cognitive function. A new treatment for stroke (and one that can reverse disability due to stroke), was first studied in rats.Are the animals used in research & education protected?: Are the animals used in research & education protected? Many federal and local laws ensure animals used in research & education are being treated humanely. These include: Animal Welfare Act Public Health Service IACUCs (committees that must approve research protocols) AAALAC (agency that accredits animal care facilities)Animal Welfare Act: Animal Welfare Act The Animal Welfare Act is a Federal law that Congress passed to protect warm-blooded animals used in research, bred for commercial sale, exhibited to the public, or commercially transported. The law requires standards of animal care to be established and enforced. The Animal Welfare Act applies to dogs, cats, primates, guinea pigs, hamsters, and farm animals. We have to be cared for properly. It’s the law!!!!!!!Animal Welfare Act : Animal Welfare Act Includes rules for mandatory surprise inspections of animal research facilities. These federal laws & regulations are in place to ensure that all research animals receive: Good veterinary care Appropriate housing Proper Feeding Humane handling Sound sanitation and ventilationDo scientists care about animals? Do they treat them well?: Do scientists care about animals? Do they treat them well? You can’t get good data from unhealthy or mis -treated animals. It is in the best interest of researchers to treat lab animals well. Most animal research facilities have a dedicated staff of people whose job is to provide daily care for the animals in their charge. Research animals must be cared for 7 days a week, 365 days a year regardless of weather or holidays.Handling of animals: Handling of animalsPowerPoint Presentation: HANDLING OF MOUSE VRD-RITM 2008PowerPoint Presentation: HANDLING OF MOUSE VRD-RITM 2008PowerPoint Presentation: HANDLING OF RAT VRD-RITM 2008PowerPoint Presentation: RABBIT VRD-RITM 2008 VRD-RITM 2008PowerPoint Presentation: GUINEA PIGS VRD-RITM 2008PowerPoint Presentation: GUINEA PIG HANDLING VRD-RITM 2008 VRD-RITM 2008 VRD-RITM 2008 Sex identification: Sex identificationPowerPoint Presentation: MALE MOUSE FEMALE MOUSEPowerPoint Presentation: VRD-RITM 2008 FEMALE RABBIT MALE RABBIT VRD-RITM 2008 VRD-RITM 2008PowerPoint Presentation: FEMALE GUINEA PIG MALE GUINEA PIG VRD-RITM 2008 VRD-RITM 2008Routes of administration: Routes of administrationPowerPoint Presentation: TAIL VEIN ADMINISTRATION VRD-RITM 2008PowerPoint Presentation: INTRAPERITONEAL ADMINISTRATION VRD-RITM 2008PowerPoint Presentation: INTRAPERITONEAL ADMINISTRATION - RATPowerPoint Presentation: INTRADERMAL ADMINISTRATION VRD-RIT`M 2008PowerPoint Presentation: SUBCUTANEOUS ADMINISTRATION VRD-RITM 2008PowerPoint Presentation: PER ORAL ADMINISTRATION VRD-RITM 2008PowerPoint Presentation: RABBIT – INTRAVENOUS (EAR VEIN) VRD-RITM 2008 VRD-RITM 2008PowerPoint Presentation: SUBCUTANEOUS INTRAMUSCULAR VRD-RITM 2008 VRD-RITM 2008PowerPoint Presentation: SUBCUTANEOUS INTRAPERITONEAL GUINEA PIG INTRADERMAL VRD-RITM 2008 VRD-RITM 2008 VRD-RITM 2008PowerPoint Presentation: GUINEA PIG ORAL ADMINISTRATION VRD-RITM 2008 VRD-RITM 2008Collection of blood samples: Collection of blood samplesPowerPoint Presentation: TAIL TIP BLOOD EXTRACTION VRD-RITM 2008PowerPoint Presentation: TAIL TIP BLOOD EXTRACTION VRD-RITM 2008PowerPoint Presentation: BLOOD COLLECTION ORBITAL BLOOD VESSELS VRD-RITM 2008 VRD-RITM 2008PowerPoint Presentation: RETRO-ORBITAL BLOOD EXTRACTION - RAT VRD-RITM 2008PowerPoint Presentation: GUINEA PIG BLOOD EXTRACTION INTRACARDIAC VRD-RITM008 VRD-RITM008PowerPoint Presentation: INSIDE FOOTWARE OUTSIDE FOOTWAREPowerPoint Presentation: MICE ROOMPowerPoint Presentation: RABBIT ROOMREQUIREMENTS IN MAKING PROPOSAL INVOLVING ANIMALS: REQUIREMENTS IN MAKING PROPOSAL INVOLVING ANIMALS Read literatures or related studies Five Freedoms Freedom from Hunger and Thirst Freedom from Physical Discomfort and Pain Freedom from Injury and Disease Freedom from Fear and Distress Freedom to Conform to Essential Behaviour Patterns Availability of species of laboratory animals Training on handling and basic procedures You do not have the permission to view this presentation. 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