The Biorepository at ILRI: Challenges and Opportunities

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Presented by Mr Absolomon Kihara, ILRI Biorepository Manager. WorldFish HQ, Penang, Malaysia, 23 Oct 2014. There is a scientific and ethical imperative to make the maximum use of biological samples which have been obtained, often at a significant expense. This encompasses the basic scientific principle that reported work should be repeatable, the need to be able to accumulate and integrate additional data around a sample as new methods and approaches become available and as more tests and analyses are conducted on the sample. It is therefore of great importance to have robust sample storage infrastructure and the associated systems for collecting, managing and sharing of data. Collectively these form a Biorepository. In this talk, I describe the Biorepository at ILRI, the challenges and opportunities presented.

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The Biorepository, Challenges and Opportunities Food for Thought Penang, Malaysia 23 rd October 2014 By Absolomon Kihara

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Steve Kemp – Team leader George Michuki Cecilia Rumberia Jason Rogena Moses Ogugo Alan Orth Sammy Kemei The team

Azizi’s Mandate:

Azizi’s Mandate Long term storage of biological material and associated metadata Support projects and programs in proper and efficient data management ( ie . design, capture, storage) Support projects and programs conducting field studies Support lab activities

Making a case for biobanking and biopreservation:

Making a case for biobanking and biopreservation Maximise the return on expensive sampling trips – use the same sample to ask multiple questions now and in the future. Maximise the understanding of the biology – different pieces of information about identical samples. Maximise the value for your study from your sample - the requirements for biobanking force improvements on your sampling methods Build a collection of well characterized samples to foster collaboration and good science

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…how it works… Not just a biorepository ….. but more of an open platform for supporting research in the region

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Make a request to store samples Agree and sign the MTA Samples are received and stored, the associated metadata is uploaded to a publicly accessible website Sign a new MTA and inform the original owner of this request Supply the material & associated metadata Azizi Biorepository http:// azizi.ilri.org / Open access workflow A request to get samples Evaluated and approved or rejected based on the original MTA Researcher ploughs back new results and findings which are also made open. An open access workflow

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opportunities a robust infrastructure

The Infrastructure:

The Infrastructure

Data management systems:

Data management systems A robust laboratory information management system for managing the samples and data, http://azizi.ilri.org/labcollector An online portal with access to open source samples and their metadata, http://azizi.ilri.org An online process for managing sample requests and approval, http://azizi.ilri.org/repository

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access to cutting edge data collection tools…

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Ng’ombe Planner a farmer centric data collection system Recording of: Production data Reproduction events Sickness Events Sickness Farmer Feedback System: Animal husbandry tips Farm management tips Reminders Notifications Retrieval of recorded data Available on all phones: Low end phones via USSD Mid tier phones using Java High end phones - Android

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Design the questionnaire as an Excel Spreadsheet Upload it to the system The system processes the Excel questionnaire Conduct the Survey Upload the survey Download the results from the system Data Cleaning & Analysis Azizi Biorepository http:// azizi.ilri.org / Open Data Kit Workflow Open Data Kit a versatile suite of data collection tools

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current collection ~400,000 well geo-referenced samples

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the collection

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the collection

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ILRI & WF Collaboration

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Biorepository Setup Aligning ILRI and WorldFish workflows and practices to create more uniform and integrated biorepositories Data collection practices LIMS system Monitoring systems

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WorldFish Biorepository Portal

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WorldFish ODK Portal

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