1 Johnson

Epidemiology of Rabies in Southeast Europe: 

Epidemiology of Rabies in Southeast Europe Nicholas Johnson Rabies and Wildlife Zoonoses Group WHO Collaborating Centre for the Characterization of Rabies and Rabies-Related Viruses

Introduction: 

Introduction Rabies is endemic within many countries of south east Europe The fox is the principal reservoir species but dog rabies cases still reported Few epidemiological studies reported from the region Identify factors that contribute to epidemiology Lack of knowledge hampers vaccination programmes

Southeast Europe (the Balkans): 

Southeast Europe (the Balkans)

The Balkan Peninsular: 

The Balkan Peninsular Turkey Bulgaria Romania Bosnia and Herzegovina Ukraine Czech Republic Greece Poland Russia Slovakia Hungary Austria Croatia Slovenia Albania Serbia Mac

Cohort Details: 

Cohort Details

Isolate details: 

Isolate details

Region of the Genome: 

Region of the Genome 327 nucleotides TTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAAAGCCCTGTATAACTCTAGGAAAGGCTCCC GATTTAAATAAAGCATACAAGTCAGTTTTATCATGCATGAGCGCCGCCAAACTTGATCCTGACGATGTATGTTCCTATTT GGCGGCGGCAATGCAGTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATCGTGATTGCACGAAAAGGAG ATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGGGCTCTGACAGGAGGCATGGAA CTGACAAGAGACCCCAC N P M G L

Phylogenetic analysis of RABV sequences from Southeastern Europe: 

Phylogenetic analysis of RABV sequences from Southeastern Europe SAD B19 Pasteur Bosnia-Herzegovina Western Turkey Romania / Russia Romania Bulgaria Eastern Turkey

Phylogenetic tree of Eastern European Isolates: 

Phylogenetic tree of Eastern European Isolates Eastern Romania / Russia

Romania: 

Romania

Bulgaria: 

Bulgaria Targovishte Dobrich Lovech Montana Pleven Lovech Former Yugoslavia Vidin Montana

Bosnia-Herzegovina: 

Bosnia-Herzegovina Bosnia-Herzegovina Hungary

Conclusions: 

Conclusions Most isolates fall into the East European group of viruses Geography dominates isolate clustering Topography may play a significant role in preventing spread of fox-rabies The Danube appears to block movement between Romania and Bulgaria Such factors could assist in future vaccination campaigns

Acknowledgements: 

Acknowledgements Co-Authors Veterinary Laboratories Agency (UK) AR Fooks FLI-Wusterhausen (Germany) T Muller, C Freuling IDT (Germany) A Vos Etlik CVRI (Turkey) O Aylan H Un Natl. Diag. & Res Vet Inst (Bulgaria) R Valtchovski Inst. Diag. & Animal Health (Romania) M Turcitu F Dumistrescu V Vlad Univ. Sarajevo (Bosnia-Hervegovina) R Velic Vet. Inst. Of Republika of Srpska V Sandrac Funding Defra (UK)