logging in or signing up Lindblad Vancouver Aug 2005 Viola Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINTLite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 381 Category: Education License: All Rights Reserved Like it (1) Dislike it (0) Added: January 23, 2008 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... By: pankajraibhu (37 month(s) ago) nice information!!!!!!!!!! Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript “BioH2” Natural & Artificial : “BioH2” Natural & Artificial Peter Lindblad Uppsala univ, SwedenSlide2: PhotoBiological Hydrogen Production Combine Photosynthesis & Hydrogen Evolution (middle) 1 - Natural Systems; Microorganisms and BioReactors (left) 2 - Artificial Systems; Chemical Lego, mimick nature (right) National project Artificial Photosynthesis -Since 10 years -45 persons -2006 at UU -Swedish Energy AgencySlide3: European project (NEST/STREP) 2005-2007, 8 partners Natural & Artificial Photo- BioH2Slide4: Future Hydrogen Society Future photobiological hydrogen production 1) Direct; Sun & Water -> H2 2) Indirect: Biomass (waste) -> H2Slide5: Future Hydrogen Society Future photobiological hydrogen production 1) Direct; Sun & Water -> H2 2) Indirect: Biomass (waste) -> H2Photobiological Hydrogen Production (Biophotolysis): Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2Photobiological Hydrogen Production (Biophotolysis): Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2 Hydrogen production, catalysed by hydrogenases; 4H+ + 4e- -> 2H2 Photobiological Hydrogen Production (Biophotolysis): Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2 Hydrogen production, catalysed by hydrogenases; 4H+ + 4e- -> 2H2 -> Green algae & Cyanobacteria Photobiological Hydrogen Production (Biophotolysis): Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2 Hydrogen production, catalysed by hydrogenases; 4H+ + 4e- -> 2H2 -> Green algae & Cyanobacteria !! No evolutionary pressure for PhotoBio-H2 !!Slide10: No H2-uptake More efficient: - H2-production - Photosynthesis - Transfer of e- No growth -> Only… Collect Solar energy, split water and produce (and evolve) H2 Slide11: Cyanobacterial enzymes directly involved in H2 metabolism Two different approaches for cyanobacterial biohydrogen: Two different approaches for cyanobacterial biohydrogen Hydrogenase based Fermentation Two different approaches for cyanobacterial biohydrogen: Two different approaches for cyanobacterial biohydrogen Nitrogenase based Two different approaches for cyanobacterial biohydrogen: Two different approaches for cyanobacterial biohydrogen Nitrogenase based e - Knock out the uptake hydrogenaseHydrogen evolution: Hydrogen evolution A: Mutant NHM5, B: Wildtype Nostoc 29133Slide18: Small scale photobioreactorUptake hydrogenase minus mutant in a small scale photobioreactor: Uptake hydrogenase minus mutant in a small scale photobioreactor Outdoors; Summer, London (UK) Sun -> Production & evolution of H2 14.9 ml H2 per h and L cells Solar energy -> H2: 0.042%, BUT per ”active cell volume” about 1%Artificial Systems: Artificial Systems Energy from the Sun; Photosynthesis -> H+ and e- from water (H20) Produce H2; Hydrogenases H+ and e- -> H2 Mimick the two processes using the smallest active parts, make small moleculesSlide21: PhotoBiological Hydrogen Production Combine Photosynthesis & Hydrogen Evolution (middle) 1 - Natural Systems; Microorganisms and BioReactors (left) 2 - Artificial Systems; Chemical Lego, mimick nature (right) National project -Since 10 years -45 persons -2006 at UU -Swedish Energy AgencySlide22: D1 D2 cp47 cp43 Fe O Ca O N O H O H H O O O O H O H H Cl 3.3Å 2.7Å 2.7Å Useful principles A SERIES OF ELECTRON- TRANSPORTERS REDOX POTENTIAL CA 1V Mn-COMPLEX FOR WATER OXIDATIONSlide23: O O N O H O H H O O O O H O H H OH to Photosynthesis Artificial Mn Mn Mn Mn Acceptor N EtO 2 C NH O N N O N N N N O O O Me Me Me Ru N N N N N Mn Mn Photosynthesis Acceptors Slide25: 3 electrons can be taken from Ru-Mn2(II,II) Magnetic field (Gauss) III,IV 2000 3000 4000 Mn II,II Dark 16 fl 35 fl MnIII,IV EPR: Ann Magnuson Ping HuangSlide26: Artificial system – permitting transfer of 3 electrons from Mn N N N N N N NH O N N O N N N N O O O Me Me Me Ru 3 electrons can be delivered by Mn 1 electron from Ru after every photon The result!Slide27: N N N N N N Ru D A LH H O 2 2 O 2 + 4H + 4H + H 2 2 Link Link hv Supramolecular Chemistry Chemical LEGO LinkSlide28: e- 2H+ H2 Electron donorSlide29: 2H+ H2 Fe Fe 2004 light Slide30: Now – a complex that can make hydrogen!! Uppsala 2004-05, Sascha Ott – Reiner Lomoth seconds 0 200 400 600 -12 -8 -4 0 turnovers 5 10 15 20 25 Hydrogen formation. Measured as charge – number of electrons utilized Background Our complex Slide31: PhotoBiological Hydrogen Production Combine Photosynthesis & Hydrogen Evolution (middle) 1 - Natural Systems; Microorganisms and BioReactors (left) 2 - Artificial Systems; Chemical Lego, mimick nature (right) National project Artificial Photosynthesis -Since 10 years -45 persons -2006 at UU -Swedish Energy AgencySlide32: Energy Agency, Knut and Alice Wallenberg’s Foundation; EU; VR Consortium for Artificial Photosynthesis 1994-2005Slide33: European project (NEST/STREP) 2005-2007, 8 partners Natural & Artificial Photo- BioH2IEA, H2-program BioHydrogen - Annex 21: IEA, H2-program BioHydrogen - Annex 21 Coordinator: Dr Jun Miyake, Japan jun-miyake@aist.go.jp 2005 - 2009 (3 + 2 years) A. H2-fermentation B. Photobiological H2production C. In vitro/Bioinspired systems D. Analyses & Acceptance 1. Processes; H2 from Sun & Water using Natural & Artifical Systems 2. Membranes, separate H2 3. Membranes, incorporate proteins and molecules : 1. Processes; H2 from Sun & Water using Natural & Artifical Systems 2. Membranes, separate H2 3. Membranes, incorporate proteins and molecules T h a n k s ! Swedish Research Council Swedish Energy Agency Nordic Energy Research Program EU/NEST: SOLAR-H: T h a n k s ! Swedish Research Council Swedish Energy Agency Nordic Energy Research Program EU/NEST: SOLAR-H You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
Lindblad Vancouver Aug 2005 Viola Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINTLite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 381 Category: Education License: All Rights Reserved Like it (1) Dislike it (0) Added: January 23, 2008 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... By: pankajraibhu (37 month(s) ago) nice information!!!!!!!!!! Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript “BioH2” Natural & Artificial : “BioH2” Natural & Artificial Peter Lindblad Uppsala univ, SwedenSlide2: PhotoBiological Hydrogen Production Combine Photosynthesis & Hydrogen Evolution (middle) 1 - Natural Systems; Microorganisms and BioReactors (left) 2 - Artificial Systems; Chemical Lego, mimick nature (right) National project Artificial Photosynthesis -Since 10 years -45 persons -2006 at UU -Swedish Energy AgencySlide3: European project (NEST/STREP) 2005-2007, 8 partners Natural & Artificial Photo- BioH2Slide4: Future Hydrogen Society Future photobiological hydrogen production 1) Direct; Sun & Water -> H2 2) Indirect: Biomass (waste) -> H2Slide5: Future Hydrogen Society Future photobiological hydrogen production 1) Direct; Sun & Water -> H2 2) Indirect: Biomass (waste) -> H2Photobiological Hydrogen Production (Biophotolysis): Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2Photobiological Hydrogen Production (Biophotolysis): Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2 Hydrogen production, catalysed by hydrogenases; 4H+ + 4e- -> 2H2 Photobiological Hydrogen Production (Biophotolysis): Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2 Hydrogen production, catalysed by hydrogenases; 4H+ + 4e- -> 2H2 -> Green algae & Cyanobacteria Photobiological Hydrogen Production (Biophotolysis): Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2 Hydrogen production, catalysed by hydrogenases; 4H+ + 4e- -> 2H2 -> Green algae & Cyanobacteria !! No evolutionary pressure for PhotoBio-H2 !!Slide10: No H2-uptake More efficient: - H2-production - Photosynthesis - Transfer of e- No growth -> Only… Collect Solar energy, split water and produce (and evolve) H2 Slide11: Cyanobacterial enzymes directly involved in H2 metabolism Two different approaches for cyanobacterial biohydrogen: Two different approaches for cyanobacterial biohydrogen Hydrogenase based Fermentation Two different approaches for cyanobacterial biohydrogen: Two different approaches for cyanobacterial biohydrogen Nitrogenase based Two different approaches for cyanobacterial biohydrogen: Two different approaches for cyanobacterial biohydrogen Nitrogenase based e - Knock out the uptake hydrogenaseHydrogen evolution: Hydrogen evolution A: Mutant NHM5, B: Wildtype Nostoc 29133Slide18: Small scale photobioreactorUptake hydrogenase minus mutant in a small scale photobioreactor: Uptake hydrogenase minus mutant in a small scale photobioreactor Outdoors; Summer, London (UK) Sun -> Production & evolution of H2 14.9 ml H2 per h and L cells Solar energy -> H2: 0.042%, BUT per ”active cell volume” about 1%Artificial Systems: Artificial Systems Energy from the Sun; Photosynthesis -> H+ and e- from water (H20) Produce H2; Hydrogenases H+ and e- -> H2 Mimick the two processes using the smallest active parts, make small moleculesSlide21: PhotoBiological Hydrogen Production Combine Photosynthesis & Hydrogen Evolution (middle) 1 - Natural Systems; Microorganisms and BioReactors (left) 2 - Artificial Systems; Chemical Lego, mimick nature (right) National project -Since 10 years -45 persons -2006 at UU -Swedish Energy AgencySlide22: D1 D2 cp47 cp43 Fe O Ca O N O H O H H O O O O H O H H Cl 3.3Å 2.7Å 2.7Å Useful principles A SERIES OF ELECTRON- TRANSPORTERS REDOX POTENTIAL CA 1V Mn-COMPLEX FOR WATER OXIDATIONSlide23: O O N O H O H H O O O O H O H H OH to Photosynthesis Artificial Mn Mn Mn Mn Acceptor N EtO 2 C NH O N N O N N N N O O O Me Me Me Ru N N N N N Mn Mn Photosynthesis Acceptors Slide25: 3 electrons can be taken from Ru-Mn2(II,II) Magnetic field (Gauss) III,IV 2000 3000 4000 Mn II,II Dark 16 fl 35 fl MnIII,IV EPR: Ann Magnuson Ping HuangSlide26: Artificial system – permitting transfer of 3 electrons from Mn N N N N N N NH O N N O N N N N O O O Me Me Me Ru 3 electrons can be delivered by Mn 1 electron from Ru after every photon The result!Slide27: N N N N N N Ru D A LH H O 2 2 O 2 + 4H + 4H + H 2 2 Link Link hv Supramolecular Chemistry Chemical LEGO LinkSlide28: e- 2H+ H2 Electron donorSlide29: 2H+ H2 Fe Fe 2004 light Slide30: Now – a complex that can make hydrogen!! Uppsala 2004-05, Sascha Ott – Reiner Lomoth seconds 0 200 400 600 -12 -8 -4 0 turnovers 5 10 15 20 25 Hydrogen formation. Measured as charge – number of electrons utilized Background Our complex Slide31: PhotoBiological Hydrogen Production Combine Photosynthesis & Hydrogen Evolution (middle) 1 - Natural Systems; Microorganisms and BioReactors (left) 2 - Artificial Systems; Chemical Lego, mimick nature (right) National project Artificial Photosynthesis -Since 10 years -45 persons -2006 at UU -Swedish Energy AgencySlide32: Energy Agency, Knut and Alice Wallenberg’s Foundation; EU; VR Consortium for Artificial Photosynthesis 1994-2005Slide33: European project (NEST/STREP) 2005-2007, 8 partners Natural & Artificial Photo- BioH2IEA, H2-program BioHydrogen - Annex 21: IEA, H2-program BioHydrogen - Annex 21 Coordinator: Dr Jun Miyake, Japan jun-miyake@aist.go.jp 2005 - 2009 (3 + 2 years) A. H2-fermentation B. Photobiological H2production C. In vitro/Bioinspired systems D. Analyses & Acceptance 1. Processes; H2 from Sun & Water using Natural & Artifical Systems 2. Membranes, separate H2 3. Membranes, incorporate proteins and molecules : 1. Processes; H2 from Sun & Water using Natural & Artifical Systems 2. Membranes, separate H2 3. Membranes, incorporate proteins and molecules T h a n k s ! Swedish Research Council Swedish Energy Agency Nordic Energy Research Program EU/NEST: SOLAR-H: T h a n k s ! Swedish Research Council Swedish Energy Agency Nordic Energy Research Program EU/NEST: SOLAR-H