Lindblad Vancouver Aug 2005

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By: pankajraibhu (37 month(s) ago)

nice information!!!!!!!!!!

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“BioH2” Natural & Artificial : 

“BioH2” Natural & Artificial Peter Lindblad Uppsala univ, Sweden

Slide2: 

PhotoBiological Hydrogen Production Combine Photosynthesis & Hydrogen Evolution (middle) 1 - Natural Systems; Microorganisms and BioReactors (left) 2 - Artificial Systems; Chemical Lego, mimick nature (right) National project Artificial Photosynthesis -Since 10 years -45 persons -2006 at UU -Swedish Energy Agency

Slide3: 

European project (NEST/STREP) 2005-2007, 8 partners Natural & Artificial Photo- BioH2

Slide4: 

Future Hydrogen Society Future photobiological hydrogen production 1) Direct; Sun & Water -> H2 2) Indirect: Biomass (waste) -> H2

Slide5: 

Future Hydrogen Society Future photobiological hydrogen production 1) Direct; Sun & Water -> H2 2) Indirect: Biomass (waste) -> H2

Photobiological Hydrogen Production (Biophotolysis): 

Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2

Photobiological Hydrogen Production (Biophotolysis): 

Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2 Hydrogen production, catalysed by hydrogenases; 4H+ + 4e- -> 2H2

Photobiological Hydrogen Production (Biophotolysis): 

Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2 Hydrogen production, catalysed by hydrogenases; 4H+ + 4e- -> 2H2 -> Green algae & Cyanobacteria

Photobiological Hydrogen Production (Biophotolysis): 

Photobiological Hydrogen Production (Biophotolysis) Photosynthesis; Capture solar energy & split H2O 2H2O -> 4H+ + 4e- + O2 Hydrogen production, catalysed by hydrogenases; 4H+ + 4e- -> 2H2 -> Green algae & Cyanobacteria !! No evolutionary pressure for PhotoBio-H2 !!

Slide10: 

No H2-uptake More efficient: - H2-production - Photosynthesis - Transfer of e- No growth -> Only… Collect Solar energy, split water and produce (and evolve) H2

Slide11: 

Cyanobacterial enzymes directly involved in H2 metabolism

Two different approaches for cyanobacterial biohydrogen: 

Two different approaches for cyanobacterial biohydrogen Hydrogenase based Fermentation

Two different approaches for cyanobacterial biohydrogen: 

Two different approaches for cyanobacterial biohydrogen Nitrogenase based

Two different approaches for cyanobacterial biohydrogen: 

Two different approaches for cyanobacterial biohydrogen Nitrogenase based e - Knock out the uptake hydrogenase

Hydrogen evolution: 

Hydrogen evolution A: Mutant NHM5, B: Wildtype Nostoc 29133

Slide18: 

Small scale photobioreactor

Uptake hydrogenase minus mutant in a small scale photobioreactor: 

Uptake hydrogenase minus mutant in a small scale photobioreactor Outdoors; Summer, London (UK) Sun -> Production & evolution of H2 14.9 ml H2 per h and L cells Solar energy -> H2: 0.042%, BUT per ”active cell volume” about 1%

Artificial Systems: 

Artificial Systems Energy from the Sun; Photosynthesis -> H+ and e- from water (H20) Produce H2; Hydrogenases H+ and e- -> H2 Mimick the two processes using the smallest active parts, make small molecules

Slide21: 

PhotoBiological Hydrogen Production Combine Photosynthesis & Hydrogen Evolution (middle) 1 - Natural Systems; Microorganisms and BioReactors (left) 2 - Artificial Systems; Chemical Lego, mimick nature (right) National project -Since 10 years -45 persons -2006 at UU -Swedish Energy Agency

Slide22: 

D1 D2 cp47 cp43 Fe O Ca O N O H O H H O O O O H O H H Cl 3.3Å 2.7Å 2.7Å Useful principles A SERIES OF ELECTRON- TRANSPORTERS REDOX POTENTIAL CA 1V Mn-COMPLEX FOR WATER OXIDATION

Slide23: 

O O N O H O H H O O O O H O H H OH to Photosynthesis Artificial Mn Mn Mn Mn Acceptor N EtO 2 C NH O N N O N N N N O O O Me Me Me Ru N N N N N Mn Mn Photosynthesis Acceptors

Slide25: 

3 electrons can be taken from Ru-Mn2(II,II) Magnetic field (Gauss) III,IV 2000 3000 4000 Mn II,II Dark 16 fl 35 fl MnIII,IV EPR: Ann Magnuson Ping Huang

Slide26: 

Artificial system – permitting transfer of 3 electrons from Mn N N N N N N NH O N N O N N N N O O O Me Me Me Ru 3 electrons can be delivered by Mn 1 electron from Ru after every photon The result!

Slide27: 

N N N N N N Ru D A LH H O 2 2 O 2 + 4H + 4H + H 2 2 Link Link hv Supramolecular Chemistry Chemical LEGO Link

Slide28: 

e- 2H+ H2 Electron donor

Slide29: 

2H+ H2 Fe Fe 2004 light

Slide30: 

Now – a complex that can make hydrogen!! Uppsala 2004-05, Sascha Ott – Reiner Lomoth seconds 0 200 400 600 -12 -8 -4 0 turnovers 5 10 15 20 25 Hydrogen formation. Measured as charge – number of electrons utilized Background Our complex

Slide31: 

PhotoBiological Hydrogen Production Combine Photosynthesis & Hydrogen Evolution (middle) 1 - Natural Systems; Microorganisms and BioReactors (left) 2 - Artificial Systems; Chemical Lego, mimick nature (right) National project Artificial Photosynthesis -Since 10 years -45 persons -2006 at UU -Swedish Energy Agency

Slide32: 

Energy Agency, Knut and Alice Wallenberg’s Foundation; EU; VR Consortium for Artificial Photosynthesis 1994-2005

Slide33: 

European project (NEST/STREP) 2005-2007, 8 partners Natural & Artificial Photo- BioH2

IEA, H2-program BioHydrogen - Annex 21: 

IEA, H2-program BioHydrogen - Annex 21 Coordinator: Dr Jun Miyake, Japan jun-miyake@aist.go.jp 2005 - 2009 (3 + 2 years) A. H2-fermentation B. Photobiological H2production C. In vitro/Bioinspired systems D. Analyses & Acceptance

1. Processes; H2 from Sun & Water using Natural & Artifical Systems 2. Membranes, separate H2 3. Membranes, incorporate proteins and molecules : 

1. Processes; H2 from Sun & Water using Natural & Artifical Systems 2. Membranes, separate H2 3. Membranes, incorporate proteins and molecules

T h a n k s ! Swedish Research Council Swedish Energy Agency Nordic Energy Research Program EU/NEST: SOLAR-H: 

T h a n k s ! Swedish Research Council Swedish Energy Agency Nordic Energy Research Program EU/NEST: SOLAR-H