logging in or signing up ELISA powerpoint Agrow knowledge Talya Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINTLite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 5400 Category: Entertainment License: All Rights Reserved Like it (4) Dislike it (0) Added: October 24, 2007 This Presentation is Public Favorites: 3 Presentation Description No description available. Comments Posting comment... By: ad871368 (3 month(s) ago) fun Saving..... Post Reply Close Saving..... Edit Comment Close By: ad871368 (3 month(s) ago) very nice Saving..... Post Reply Close Saving..... Edit Comment Close By: datalajay (13 month(s) ago) Nice information! Saving..... Post Reply Close Saving..... 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Edit Comment Close Premium member Presentation Transcript Slide2: ELISA Immuno Explorer™ Kit Instructors: Stan Hitomi Director, Edward Teller Education Center UC Davis / Lawrence Livermore National Laboratory, Livermore, CA Kirk Brown Lead Instructor, Edward Teller Education Center Science Chair, Tracy High School and Delta College, Tracy, CAELISA Enzyme-Linked Immunosorbant Assay: ELISA Enzyme-Linked Immunosorbant Assay Mammalian immune system Antibody specificity Biology’s “magic bullet” Evolved over millions of years Harness nature’s tool kit Imagine the applications!Links to the Real World: Links to the Real World Mad Cow Disease, SARS, HIV GMO Drug and steroid testing Pregnancy / Reproduction Biodefense Cancer treatment ELISA Immuno Explorer Kit Features: ELISA Immuno Explorer Kit Features Lab completed in a 45 min period Supplies for 48 students (12 workstations) Comprehensive and flexible curriculum Compelling real-world links Cost effective Classroom Safe Striking resultsWhat Can You Teach With The ELISA Kit?: What Can You Teach With The ELISA Kit? Hands-on Immunology One lesson integrates multiple standards Environmental Science – surveillance Immunology – antibody/antigen interactions Disease/Health Science – infection, detection, transmission Biowarfare – defense against acts of aggression Links to research, agriculture, medicine, and consumer products Modern way to teach classic and contemporary science Teach more with less!Immune Response: Immune Response A. Pathogen C. Macrophage D. Macrophage E. Macrophage F. T cell B. B cells G. B cell H. Memory B cells I. Plasma cells J. Antibodies attach to pathogenELISA Antibody Structure: ELISA Antibody StructureSlide9: ELISA ANIMATIONELISA Enzyme-Linked Immunosorbant Assay: ELISA Enzyme-Linked Immunosorbant AssayELISA Kit Workstation Inventory: ELISA Kit Workstation Inventory Reagents: Yellow tubes Test samples 2 Violet tube (+) Positive control 1 Blue tube (-) Negative control 1 Green tube (PA) Primary antibody 1 Orange tube (SA) Secondary antibody 1 Lab Equipment and Supplies: Microplate strips, pipettor, pipette tips, transfer pipette, wash buffer, paper towels, marking pen ELISA Kit Quick Guide: ELISA Kit Quick GuideELISA Kit Results: ELISA Kit ResultsWhat Are The Reagents? And What Function Do They Perform?: What Are The Reagents? And What Function Do They Perform? PBS: Phosphate buffered saline – provides stable buffered environment to maintain antibody structure Tween 20: Nonionic detergent – removes non-specifically bound proteins to reduce background and blocks protein binding sites on the polystyrene Microplates: Polystyrene – proteins absorb (bind) by hydrophobic bonds to the polystyrene What Are The Reagents? And What Function Do They Perform?: What Are The Reagents? And What Function Do They Perform? Antigen: Chicken gamma globulin Primary antibody: Polyclonal anti-chicken antibody made by rabbits Secondary antibody: Polyclonal anti-rabbit antibody made by goats linked (conjugated) to horseradish peroxidase (HRP) Enzyme substrate: 3,3’,5,5’ – tetramethylbenzidine (TMB) – a colorless solution that when oxidized by HRP turns blueELISA Kit Protocol Issues: ELISA Kit Protocol Issues Controls: Protocol II Positive control = antigen Negative control = PBS Triplicate samples: controls against cross contamination (2 out of 3 ain’t bad) Washing: eliminates non-specific binding and reduces background Ways The ELISA Kit Can Be Used: Ways The ELISA Kit Can Be UsedSlide18: ELISA test for Transmissible Spongiform Encephalopathies (TSEs) a PrPres Proteinase K resistant Aggregates in detergent PrPsens Proteinase K sensitive Soluble in detergent b Prion Proteins (PrPres and PrPsens) Uses differences in diseased prions vs. normal prions to prepare sample. Proteinase K only digests normal, not diseased, prions . ELISA tests for any prion proteinSlide19: Sample brain tissue Homogenize brain tissue Digest with Proteinase K (normal prions are digested, diseased prions are resistant) Concentrate Denature Proteinase K Perform ELISA TSE test sample preparationSlide20: Protocol II: Antigen Detection ELISA Protocol - ELISA on simulated animal brain samples Real-World Application – TSE TestReal-world Applications of Antibodies: Real-world Applications of Antibodies Agricultural Uses – Crop-specific disease diagnosis – Animal disease diagnosis – Detection of GM crops – Basic research Applications – Dipstick tests/ELISA – Immunostaining – Western blotting Bio-Rad’s TSE ELISA KitExample: Pregnancy Test: Example: Pregnancy Test You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
ELISA powerpoint Agrow knowledge Talya Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINTLite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 5400 Category: Entertainment License: All Rights Reserved Like it (4) Dislike it (0) Added: October 24, 2007 This Presentation is Public Favorites: 3 Presentation Description No description available. Comments Posting comment... By: ad871368 (3 month(s) ago) fun Saving..... Post Reply Close Saving..... Edit Comment Close By: ad871368 (3 month(s) ago) very nice Saving..... Post Reply Close Saving..... Edit Comment Close By: datalajay (13 month(s) ago) Nice information! Saving..... Post Reply Close Saving..... Edit Comment Close By: binita_keshab (13 month(s) ago) nice Saving..... Post Reply Close Saving..... Edit Comment Close By: khalidmuayad (14 month(s) ago) thank you Saving..... Post Reply Close Saving..... Edit Comment Close Premium member Presentation Transcript Slide2: ELISA Immuno Explorer™ Kit Instructors: Stan Hitomi Director, Edward Teller Education Center UC Davis / Lawrence Livermore National Laboratory, Livermore, CA Kirk Brown Lead Instructor, Edward Teller Education Center Science Chair, Tracy High School and Delta College, Tracy, CAELISA Enzyme-Linked Immunosorbant Assay: ELISA Enzyme-Linked Immunosorbant Assay Mammalian immune system Antibody specificity Biology’s “magic bullet” Evolved over millions of years Harness nature’s tool kit Imagine the applications!Links to the Real World: Links to the Real World Mad Cow Disease, SARS, HIV GMO Drug and steroid testing Pregnancy / Reproduction Biodefense Cancer treatment ELISA Immuno Explorer Kit Features: ELISA Immuno Explorer Kit Features Lab completed in a 45 min period Supplies for 48 students (12 workstations) Comprehensive and flexible curriculum Compelling real-world links Cost effective Classroom Safe Striking resultsWhat Can You Teach With The ELISA Kit?: What Can You Teach With The ELISA Kit? Hands-on Immunology One lesson integrates multiple standards Environmental Science – surveillance Immunology – antibody/antigen interactions Disease/Health Science – infection, detection, transmission Biowarfare – defense against acts of aggression Links to research, agriculture, medicine, and consumer products Modern way to teach classic and contemporary science Teach more with less!Immune Response: Immune Response A. Pathogen C. Macrophage D. Macrophage E. Macrophage F. T cell B. B cells G. B cell H. Memory B cells I. Plasma cells J. Antibodies attach to pathogenELISA Antibody Structure: ELISA Antibody StructureSlide9: ELISA ANIMATIONELISA Enzyme-Linked Immunosorbant Assay: ELISA Enzyme-Linked Immunosorbant AssayELISA Kit Workstation Inventory: ELISA Kit Workstation Inventory Reagents: Yellow tubes Test samples 2 Violet tube (+) Positive control 1 Blue tube (-) Negative control 1 Green tube (PA) Primary antibody 1 Orange tube (SA) Secondary antibody 1 Lab Equipment and Supplies: Microplate strips, pipettor, pipette tips, transfer pipette, wash buffer, paper towels, marking pen ELISA Kit Quick Guide: ELISA Kit Quick GuideELISA Kit Results: ELISA Kit ResultsWhat Are The Reagents? And What Function Do They Perform?: What Are The Reagents? And What Function Do They Perform? PBS: Phosphate buffered saline – provides stable buffered environment to maintain antibody structure Tween 20: Nonionic detergent – removes non-specifically bound proteins to reduce background and blocks protein binding sites on the polystyrene Microplates: Polystyrene – proteins absorb (bind) by hydrophobic bonds to the polystyrene What Are The Reagents? And What Function Do They Perform?: What Are The Reagents? And What Function Do They Perform? Antigen: Chicken gamma globulin Primary antibody: Polyclonal anti-chicken antibody made by rabbits Secondary antibody: Polyclonal anti-rabbit antibody made by goats linked (conjugated) to horseradish peroxidase (HRP) Enzyme substrate: 3,3’,5,5’ – tetramethylbenzidine (TMB) – a colorless solution that when oxidized by HRP turns blueELISA Kit Protocol Issues: ELISA Kit Protocol Issues Controls: Protocol II Positive control = antigen Negative control = PBS Triplicate samples: controls against cross contamination (2 out of 3 ain’t bad) Washing: eliminates non-specific binding and reduces background Ways The ELISA Kit Can Be Used: Ways The ELISA Kit Can Be UsedSlide18: ELISA test for Transmissible Spongiform Encephalopathies (TSEs) a PrPres Proteinase K resistant Aggregates in detergent PrPsens Proteinase K sensitive Soluble in detergent b Prion Proteins (PrPres and PrPsens) Uses differences in diseased prions vs. normal prions to prepare sample. Proteinase K only digests normal, not diseased, prions . ELISA tests for any prion proteinSlide19: Sample brain tissue Homogenize brain tissue Digest with Proteinase K (normal prions are digested, diseased prions are resistant) Concentrate Denature Proteinase K Perform ELISA TSE test sample preparationSlide20: Protocol II: Antigen Detection ELISA Protocol - ELISA on simulated animal brain samples Real-World Application – TSE TestReal-world Applications of Antibodies: Real-world Applications of Antibodies Agricultural Uses – Crop-specific disease diagnosis – Animal disease diagnosis – Detection of GM crops – Basic research Applications – Dipstick tests/ELISA – Immunostaining – Western blotting Bio-Rad’s TSE ELISA KitExample: Pregnancy Test: Example: Pregnancy Test