3939s1 Buchanan

Resistance – Thermal and Other : 

Resistance – Thermal and Other Robert L. Buchanan DHHS Food and Drug Administration Center for Food Safety and Applied Nutrition

Thermal Resistance of E. sakazakii – Laboratory Trials: 

Thermal Resistance of E. sakazakii – Laboratory Trials Nazarowec-White and Farber (1997) Open stainless steel tubes in constant temperature water bath 5 pooled food strains and 5 pooled clinical strains Edelson-Mammel and Buchanan (2003) Submerged coil apparatus 12 individual strains – food and clinical

Thermal Resistance : 

Thermal Resistance D-Value: Time at a given temperature needed to reduce a microbial population by 90% Z-Value: Change in temperature needed to change D-value by 90%

Thermal Death Time Curves for 2 Enterobacter sakazakii Strains Heated at 58C: 

Thermal Death Time Curves for 2 Enterobacter sakazakii Strains Heated at 58C D = 30.5 sec D = 591.9 sec

Distribution of D58°C-values for 12 Enterobacter sakazakii strains: 

Distribution of D58°C-values for 12 Enterobacter sakazakii strains

Comparison of D58°C-Values for Different Enterobacteriaceae: 

Comparison of D58°C-Values for Different Enterobacteriaceae

Effect of Heating Temperature on D-Value for E. sakazakii 607: 

Effect of Heating Temperature on D-Value for E. sakazakii 607

Reported Z-values: 

Reported Z-values Nazarowec-White and Farber (1997) Pooled food isolates: 5.6°C Pooled clinical isolates: 6.0 °C All: 5.8 °C Edelson-Mammel and Buchanan (2003) Strain 607: 5.6 °C

Predicted Inactivation: 

Predicted Inactivation Results of these heating trials indicate that heating rehydrated infant formula at 70°C for even a few seconds will result in a substantial inactivation of E. sakazakii

Effect of Temperature of Water Used to Rehydrate Infant Formula: 

Effect of Temperature of Water Used to Rehydrate Infant Formula Inoculated dried infant formula in baby bottle Add water pre-heated to different temperatures Cap and agitate periodically for 10 minutes Analyze rehydrated formula for E. sakazakii

Temperature Decline During Rehydration of Infant Formula: 

Temperature Decline During Rehydration of Infant Formula

Rehydration of Dried Infant Formula: 

Rehydration of Dried Infant Formula Lower Limit of Detection

Impact of Heating on Nutrient Content: 

Impact of Heating on Nutrient Content Conducted by Atlanta Center for Nutrient Analysis (ACNA) Used boiling water as the worst case and compared against control Analyses done in triplicate Results expressed in terms of units/100 cal

Impact of Heating on Nutrient Content: 

Impact of Heating on Nutrient Content

Impact of Heating on Nutrient Content: 

Impact of Heating on Nutrient Content

Impact of Heating on Nutrient Content: 

Impact of Heating on Nutrient Content

Thermal Resistance of E. sakazakii – Pilot Plant: 

Thermal Resistance of E. sakazakii – Pilot Plant Nazarowec-White et al. (1999) Pooled strains? HTST pasteurizer

Effectiveness of Pasteurization: 

Effectiveness of Pasteurization

Resistance to Other Treatments: 

Resistance to Other Treatments Great deal of information available about Enterobacteriaceae in general

Resistances If Like Other Enterobacteriaceae: 

Resistances If Like Other Enterobacteriaceae Not heat resistant Moderate acid resistance if adapted Moderate alkali resistance if adapted Low to moderate chlorine resistance Low to moderate irradiation resistance Will remain viable in refrigerated and frozen products for extended periods, particularly if neutral pH Moderate to good resistance to drying

Resistance to Other Treatments: 

Resistance to Other Treatments Little published information available specifically about E. sakazakii Even less available on distribution of resistances

Other Resistances: 

Other Resistances Isolation from dried foods indicates resistance to drying Isolation from seeds treated with hypochlorite suggest that at least some strains may be relatively resistant to chlorine (Okuda et al., 1994) Relatively sensitive to the growth inhibiting effects of chitosans (No et al., 2002)

Resistance to Dehydration: 

Resistance to Dehydration

Summary: 

Summary Not a particularly thermally resistant microorganism Substantial diversity in thermal resistance among strains Good agreement among studies Inactivation at temperatures above 70°C, even for a few seconds Specific information on its resistance to other treatments is generally lacking