Slide1: NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY -an ideal tool for Diagnosis & Drug Design B. HASEENA BANU Asst.Professor,SriPadmavathi School of Pharmacy,Tiruchanoor,Tirupati More free powerpoints at http://www.worldofteaching.com


CONTENTS: CONTENTS INTRODUCTION BASIC PRINCIPLE DIFFERENT TECHNIQUES APPLICATIONS OF NMR IN MEDICINE CLINICAL APPLICATIONS IN DIAGNOSIS DRUG DEVELOPMENT NMR Nobel prize laureates


INTRODUCTION: INTRODUCTION BASIC PRINCIPLE IN NMR Atomic nuclei – tiny magnets In external magnetic field - allign or oppose If irradiated with EMR of proper frequency – Resonance √ α βo PARAMETERS Chemical shifts Spin lattice relaxation time


DIFFERENT TECHNIQUES: DIFFERENT TECHNIQUES Based on nuclei H1 NMR C13 NMR F13 NMR & P31 NMR N15 NMR & O17 NMR CWNMR FTNMR 2D_NMR &3D_NMR COSY _CORRELATION SPECTROSCOPY MRI_MAGNETIC RESONANCE IMAGING ESR_ELECTRON SPIN RESONANCE SPECTROSCOPY


APPLICATIONS OF NMR IN MEDICINE: APPLICATIONS OF NMR IN MEDICINE CLINICAL APPLICATION OF PROTON IMAGING IN DIAGNOSIS BRAIN Distinguishing gray matter & white matter Imaging posterior fossae, brain stem, spinal cord Detect demyelinating lesions, tumors, hemorrhages, infarctions


Slide6: ABDOMEN Metabolic liver disease Measures liver iron over load in hemochromatosis Focal areas of inflammation in chronic active hepatisis KIDNEYS Distinguishing renal cortex & medulla To evaluate transplanted kidney PELVIS Differentiates between Benign prostatic hyperplasia & prostatic carcinoma Detects bladder tumours


Slide7: MEDIASTINUM, HILUM, LUNG Assessing abnormalities Respiratory gating HEART Tomographic images of heart muscle, chambers, valvular structures ECHO-PLANAR TECHNIQUE Discrimination between infarcted, ischemic & normal myocardium BREAST 3D-NMR & single-slice planar imaging in detecting breast abnormalities


Slide8: MUSCULO SKELETAL SYSTEM Demonstrates Osteo myelitis, tumor metastasis in vertebral bodies & pelvic bones Images of muscles, tendons, ligaments BLOOD VESSELS & FLOW Atherosclerotic vascular disease Assess blood flow in major vessels. INVIVO SPECTROSCOPY Chemical shift phenomenon Diagnosis of rare disease to inborn errors- Mc Ardle’s syndrome INVIVO Analysis of Bone flouride content.


PUTTING NMR IMAGING INTO PERSPECTIVE: PUTTING NMR IMAGING INTO PERSPECTIVE Eliminates risk of x-radiation Excellent spatial & contrast resolution Detecting diseases at earlier stages


NMR IN PHARMACEUTICAL RESEARCH: NMR IN PHARMACEUTICAL RESEARCH Leading technology for 3-D structure determination of bio-macromolecules Studying protein structure SAR by NMR – Novel lead compounds Chemical shift mapping – Structural information on the binding modes and site positions Molecular dynamics, conformational analysis SHAPES NMR – SOLVE


Slide11: Structural genomics Identifying gene products in disease Targets of drug design. Development of Novel drug delivery systems Enhancing HTS assays Thousands of compounds Cryogenic NMR technology / cryoprobes


NMR Nobel Prize Laureates: NMR Nobel Prize Laureates The Nobel Prize in Physics 1943, Ohostern, USA – Discovery of magnetic moment of proton The Nobel Prizein Physics 1952- IsidorJ. Rafi,USA – Discovery of new methods for nuclear magnetic precision The Nobel Prize in Chemistry 1991 – Felix Bloch, USA and Edward M. Purcell, USA – Methodology of high resolution NMR. The Nobel Prize in Chemistry 2002 – Kurt Wuthrich, Switzerland – Determining 3-D Structure by NMR. The Nobel Prize in Medicine 2003 – Paul C. Lauterbur, USA and Peter Mans field, U.K. – Discoveries concerning MRI.


THANKING YOU: THANKING YOU