logging in or signing up cloning Simeone Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINTLite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 20639 Category: Education License: All Rights Reserved Like it (13) Dislike it (0) Added: January 15, 2008 This Presentation is Public Favorites: 4 Presentation Description No description available. Comments Posting comment... By: asifmalik (7 month(s) ago) i want this presentation Saving..... Post Reply Close By: fafar (6 month(s) ago) hi very good Saving..... Edit Comment Close By: joinsu (9 month(s) ago) hi, i want to download this document, please give access Saving..... Post Reply Close Saving..... 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See all Premium member Presentation Transcript Cloning: Cloning Dr. Abdelsalam Talafha, DVM Diplomate, American College of TheriogenologistsCloning: Cloning Production of genetically identical individuals that have identical nuclear DNACloning Technologies: Cloning Technologies Recombinant DNA technology DNA cloning Molecular cloning Gene cloning Reproductive cloning Therapeutic cloning Embryo cloningDNA Cloning: DNA Cloning Transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid Plasmids Self-replicating extra-chromosomal circular DNA molecules, distinct from normal bacterial genomeDNA Cloning - Uses: DNA Cloning - Uses Gene therapy Genetic engineering of organisms Genome sequencingReproductive Cloning : Reproductive Cloning A technology used to generate an animal that has same nuclear DNA as another currently or previously existing animal Dolly How Is Reproductive Cloning Done? Somatic cell nuclear transfer (SCNT)SCNT: SCNT Starts with removal of polar body and chromosomes from an oocyte Enucleated oocyte Donor cell then inserted into perivitelline space of enucleated oocyteSCNT: SCNT Oocyte and donor cell are fused and activated by an electric pulse to begin cell division Developed embryos transferred to surrogate females Birth of an individualSCNT: SCNT Sources of somatic cells Cell from individual Cells grown in culture Frozen tissueTherapeutic Cloning: Therapeutic Cloning Production of human embryos for use in research Goal To harvest stem cells that can be used to study human development and to treat diseaseTherapeutic Cloning: Therapeutic Cloning Stem cells Cells have ability to divide and give rise to both specialized cells and more stem cells Derived from Adults Preimplantation embryos (Embryonic stem cells)Stem Cells: Stem Cells Replacement cells to treat Heart disease Alzheimer's Cancer Diabetes Parkinson's disease Spinal cord injurySCNT- Potential Biotechnological Applications: SCNT- Potential Biotechnological Applications Harvesting donor cells from transgenic animal Genetic modification of cultured donor cells prior to nuclear transfer Producing genetically modified cloned offspringGenetically Modified Cloned Offspring: Genetically Modified Cloned Offspring Biotechnological applications Production of pharmaceuticals Xenotransplantation Study and eradication of human disease Improvement of livestockProduction Of Pharmaceuticals: Production Of Pharmaceuticals Gene expressing human coagulation factor IX introduced into ovine fetal fibroblasts linked to another gene with a high level of expression in mammary gland Protein expressed in milkProduction Of Pharmaceuticals: Production Of Pharmaceuticals Insulin for diabetes Interferon for viral infections Tissue plasminogen activator (which dissolves blood clots)Xenotransplantation: Xenotransplantation Aim: To develop animals whose organs will not cause an immunological response and destroy transplanted tissue when transferred to humansXenotransplantation: Xenotransplantation Pig organs Hearts, lungs, kidneys, liver Neural tissue for Parkinson's Islets cells for diabetes patientsStudy Human Disease: Study Human Disease Sheep model to investigate human cystic fibrosis Cloned sheep used for drug testing and to evaluate new therapiesImprovement Of Livestock: Improvement Of Livestock Cloning animal with excellent traits Production of a large number of clones from high quality animals Allow overall genetic improvement of herd Repopulate endangered animalsAnimal Cloning: Animal Cloning Sheep, cattle, goats, pigs, and mice Cloning efforts in rabbits, rats, cats, dogs, and horses are ongoing Risks Of Cloning: Risks Of Cloning Reproductive cloning expensive and highly inefficient > 90% of cloning attempts fail to produce viable offspringRisks Of Cloning: Risks Of Cloning High rate of fetal loss during pregnancy Compromised immune function Higher rates of infection Tumor growth Early neonatal death Abnormally large at birth Die mysteriouslyRisks Of Cloning: Risks Of Cloning Cloned fetuses have abnormalities Abnormal placentation Pregnancy toxemia Hydroallantois Should Humans Be Cloned?: Should Humans Be Cloned? Due to Inefficiency of animal cloning Lack of understanding about reproductive cloning Risks of cloning Should Humans Be Cloned?: Should Humans Be Cloned? Unethical to attempt to clone humans Same problems would be expected in human cloning We do not know how cloning could impact mental development Should Humans Be Cloned?: Should Humans Be Cloned? High risk to health of fetus or infant and mother Psychological risks for mother as a result of Late spontaneous abortions Birth of a stillborn child Birth of a child with severe health problems You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
cloning Simeone Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINTLite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 20639 Category: Education License: All Rights Reserved Like it (13) Dislike it (0) Added: January 15, 2008 This Presentation is Public Favorites: 4 Presentation Description No description available. Comments Posting comment... By: asifmalik (7 month(s) ago) i want this presentation Saving..... Post Reply Close By: fafar (6 month(s) ago) hi very good Saving..... Edit Comment Close By: joinsu (9 month(s) ago) hi, i want to download this document, please give access Saving..... Post Reply Close Saving..... Edit Comment Close By: harishshk (11 month(s) ago) Hi, I want to download this document. Please give access. Saving..... Post Reply Close Saving..... Edit Comment Close By: chandrakant124 (11 month(s) ago) hey plz send me ppt of gene cloning on my email -id is chandrakantborhade@ymail.com Saving..... Post Reply Close Saving..... Edit Comment Close By: algaily1 (12 month(s) ago) http://www.authorstream.com/Presentation/Simeone-41667-cloni.. Saving..... Post Reply Close Saving..... Edit Comment Close loading.... See all Premium member Presentation Transcript Cloning: Cloning Dr. Abdelsalam Talafha, DVM Diplomate, American College of TheriogenologistsCloning: Cloning Production of genetically identical individuals that have identical nuclear DNACloning Technologies: Cloning Technologies Recombinant DNA technology DNA cloning Molecular cloning Gene cloning Reproductive cloning Therapeutic cloning Embryo cloningDNA Cloning: DNA Cloning Transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid Plasmids Self-replicating extra-chromosomal circular DNA molecules, distinct from normal bacterial genomeDNA Cloning - Uses: DNA Cloning - Uses Gene therapy Genetic engineering of organisms Genome sequencingReproductive Cloning : Reproductive Cloning A technology used to generate an animal that has same nuclear DNA as another currently or previously existing animal Dolly How Is Reproductive Cloning Done? Somatic cell nuclear transfer (SCNT)SCNT: SCNT Starts with removal of polar body and chromosomes from an oocyte Enucleated oocyte Donor cell then inserted into perivitelline space of enucleated oocyteSCNT: SCNT Oocyte and donor cell are fused and activated by an electric pulse to begin cell division Developed embryos transferred to surrogate females Birth of an individualSCNT: SCNT Sources of somatic cells Cell from individual Cells grown in culture Frozen tissueTherapeutic Cloning: Therapeutic Cloning Production of human embryos for use in research Goal To harvest stem cells that can be used to study human development and to treat diseaseTherapeutic Cloning: Therapeutic Cloning Stem cells Cells have ability to divide and give rise to both specialized cells and more stem cells Derived from Adults Preimplantation embryos (Embryonic stem cells)Stem Cells: Stem Cells Replacement cells to treat Heart disease Alzheimer's Cancer Diabetes Parkinson's disease Spinal cord injurySCNT- Potential Biotechnological Applications: SCNT- Potential Biotechnological Applications Harvesting donor cells from transgenic animal Genetic modification of cultured donor cells prior to nuclear transfer Producing genetically modified cloned offspringGenetically Modified Cloned Offspring: Genetically Modified Cloned Offspring Biotechnological applications Production of pharmaceuticals Xenotransplantation Study and eradication of human disease Improvement of livestockProduction Of Pharmaceuticals: Production Of Pharmaceuticals Gene expressing human coagulation factor IX introduced into ovine fetal fibroblasts linked to another gene with a high level of expression in mammary gland Protein expressed in milkProduction Of Pharmaceuticals: Production Of Pharmaceuticals Insulin for diabetes Interferon for viral infections Tissue plasminogen activator (which dissolves blood clots)Xenotransplantation: Xenotransplantation Aim: To develop animals whose organs will not cause an immunological response and destroy transplanted tissue when transferred to humansXenotransplantation: Xenotransplantation Pig organs Hearts, lungs, kidneys, liver Neural tissue for Parkinson's Islets cells for diabetes patientsStudy Human Disease: Study Human Disease Sheep model to investigate human cystic fibrosis Cloned sheep used for drug testing and to evaluate new therapiesImprovement Of Livestock: Improvement Of Livestock Cloning animal with excellent traits Production of a large number of clones from high quality animals Allow overall genetic improvement of herd Repopulate endangered animalsAnimal Cloning: Animal Cloning Sheep, cattle, goats, pigs, and mice Cloning efforts in rabbits, rats, cats, dogs, and horses are ongoing Risks Of Cloning: Risks Of Cloning Reproductive cloning expensive and highly inefficient > 90% of cloning attempts fail to produce viable offspringRisks Of Cloning: Risks Of Cloning High rate of fetal loss during pregnancy Compromised immune function Higher rates of infection Tumor growth Early neonatal death Abnormally large at birth Die mysteriouslyRisks Of Cloning: Risks Of Cloning Cloned fetuses have abnormalities Abnormal placentation Pregnancy toxemia Hydroallantois Should Humans Be Cloned?: Should Humans Be Cloned? Due to Inefficiency of animal cloning Lack of understanding about reproductive cloning Risks of cloning Should Humans Be Cloned?: Should Humans Be Cloned? Unethical to attempt to clone humans Same problems would be expected in human cloning We do not know how cloning could impact mental development Should Humans Be Cloned?: Should Humans Be Cloned? High risk to health of fetus or infant and mother Psychological risks for mother as a result of Late spontaneous abortions Birth of a stillborn child Birth of a child with severe health problems