WP 2 6 1 IgE SANAS van Drunen

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Objective of workpackage: 

Objective of workpackage To better understand the genetic, molecular and cellular mechanisms of IgE function.

Simplistic view on allergy : 

Simplistic view on allergy First allergen exposure Second allergen exposure However : IgE ≠ symptoms

Slide3: 

Study of asymptomatic subjects and subjects with sensitization to Parietaria or birch pollen, a pilot study GA2LEN Network WP 2.6

Potential mechanisms targeted : 

Potential mechanisms targeted Cross linking of FcγRII on basophil Regulatory dendritic cell or T lymphocyte Genotype versus Phenotype

Cross linking of FcγRII: 

Cross linking of FcγRII Andrew Saxon IgE - IgG fusion Allergen - IgG fusion ITAM versus ITIM Functional differences Genetic differences

Regulatory functions: 

Regulatory functions DC required for effector phase DC function and regulatory T lymphocyte Functional differences Genetic differences

SANAS (design 1): 

SANAS (design 1) Enrol 160 - 200 subjects 4 groups Sensitised to Birch pollen symptomatic asymptomatic Sensitised to Parietaria symptomatic asymptomatic

SANAS (design 2): 

SANAS (design 2) Questionaires Skin prick test Serum samples IgE and IgG4 determination Basophils (active/passive) T regulatory cells

Birch patients: 

Birch patients Clinical Groups Fokkens (Amsterdam) Bachert (Ghent) Kowalski (Lodz) Carlsen (Oslo) - Children Van Hage (Stockholm) Valenta (Vienna)

Birch analysis (on site): 

Birch analysis (on site) Basophil activation status Fokkens (Amsterdam) Kowalski (Lodz) Regulatory T lymphocytes Fokkens (Amsterdam) Van Hage (Stockholm)

Parietaria patients: 

Parietaria patients Clinical Groups Papadopoulous (Athens) - Children Todo-Bom (Coimbra) Passalacqua (Genua) Colombo (Palermo) Bresciani (Rome)

Parietaria analysis (on site): 

Parietaria analysis (on site) Basophil activation status Colombo (Palermo) Bresciani (Rome)

Central laboratories: 

Central laboratories Recombinant serum IgE and IgG van Ree (Amsterdam) Indirect Basophil cell line sensibilization Maurer (Berlin) – Parietaria Dauron (Paris) – Birch Coordination regulatory T lymphocytes Akdis (Zurich/Davos) Staph-related IgE Bachert

Future prospects and food for thought: 

Future prospects and food for thought

Clinical observations: 

Clinical observations Pets in childhood IgE and cows milk Thanksgivings disease Asymptomatic Symptomatic

Clinical observations: 

Clinical observations Pets in childhood IgE and cows milk Thanksgivings disease Asymptomatic Symptomatic

Conversion to asymptomatic: 

Conversion to asymptomatic Immunotherapy and modulators PGE2 Vitamine D3 Stable dust Small interference molecules ITAM and ITIM Regulatory Cells

Slide18: 

Thank you for your attention Coimbra Amsterdam Lodz Athens Paris Rome Berlin Genua Stockholm Ghent Davos Palermo Oslo Vienna