PPTFP18 Konstantinova

Multigene phylogenetic analysis of lower metazoans Konstantinova A.V., Nikitin M.A. Post-graduate students of the faculty of bioengeneering and bioinformatics, M.V.Lomonosov Moscow State University : 

Multigene phylogenetic analysis of lower metazoans Konstantinova A.V., Nikitin M.A. Post-graduate students of the faculty of bioengeneering and bioinformatics, M.V.Lomonosov Moscow State University

There are three main questions in animal phylogeny:: 

There are three main questions in animal phylogeny: What taxon is closer to multicellular ancestor? What grouping of bilaterian phyla are monophyletic ? What organisms are the nearest to multicellular ones ?

Who is older?: 

Who is older? www.ibss.iuf.net/blacksea/species/freelife/ctenophora/mnemleid.html www.uib.no/info/bilder/2005/nematostella.jpg

Our strategy:: 

Our strategy:

Slide5: 

Here are some bases, where we took Lower metazoan`s amino acids:

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We searched the same genes (which were seek in Ctenophora) in Animals, Fungi, Plantae.

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Through gene`s searching we also looked for orthologs of these genes among species. orthologs ?

Slide8: 

Monosigaov RYVAAYLLATLGGNATPSEADVKKILSSVGIEADQAKLTKVLGELKGKDLAALIAEGEKKLASVPAAGAAPAAAAPAAAAAAPAA-KAEKKEEKKKEEKPPS Cte_______ RYVAAYLLAALGGNASPSADDIKNILSAVGVSVDDEKLSFVMGQLKGKDINEVVASGIGKLASVPSGGAVAASSGGAAAAGAAAAPAEEEKAP--EESEEES Reniera___ RYVAAYLLASLGSSDSPTKEDITKILESVGLEIEDDQLDKVISELKGKDINDVIAEGMGKLASIPSGGVAAASGGGGAAAGGGDGGEEEKKEEKKEESEEES Suberites_ RYVAAYLLASLGGEGCPSKEKIAKILESVGIDVSDERLSKVMSELKGKDVSEVIADGMGKLASMPSGGAAASSGGGG---AGGDAPAEEKKEEKKEESEEES Trichoplax XYVSAYLLARLGGNDSPSASDIESILGSVGIDVDSXRLSKVISELEGKDVDEVIKXGLSKLASXPSGXAX--SSGAGASAGGGAAP-EAEKEEKPEEEEEES Hydram____RYVAAYLLATLGGNASPSENDLKKILESVGVGYDATRASTVVKQLKGKSLPDLIAEGSKKMASMPSGGAAAPSAVAAAAPAGGAAPA-AKAEEKKEESEEES Podocoryne RYVSAYLLATLGGNNSPSESDIKAILESVGVSYDAERAKTVVAQLKGKSLPELIEEGSKKMASMPSGGAAPAAGGAVA--SGGDAAQEEKKEEKKEESEEES Nematostel RYVAAYLLATLGNNKNPSAKDIKGILDSVGIESDMERLNKVISELSGKSVDEIIQAGKSKLATVPTGGAVAAGGAPAAAAAGGDAKAEEKKEEKKAESEEES Acropora__ RYVAAYLLATLGGNENPSASDIKDILSSVGIDVDSERLDVVIKELSGKNVNEVIAAGKSKLASVPTGGAVMAGAAAASSSAGGGGGSEEKGEEKEEEEEEES Schistosoj RYLAAYLLAQLGGTGRPQESDIKNILSSVGVECEQERAKLGVDQLHGKNVRDLINTGKEKMSAVPSGGAPAAATAAAEAPKGGDKAAAPPKEEKKEES-EES Schmidtea_ RYLSSYIL-SVLGGNAEPEAEDISTILTAVGIECDINKAKEAVQAIDCRDVNDLISQGLKKISSVPTGGAGAAPTAATSAP--APAAKEEVKAAAKESSSESE Argopecten RYVAAYLLASLGGNASPSAADLEKIIGSVGIEAEADKIKKIISELKGKNIEELIAAGTAKLASVPSGGGGGAPAAGGAAP-AADEKKEEKKEEKKKEESEES Aplysia___ RYVAAYLLCALGGNSSPSAAQIEKILSSVGIEAESDKINKIIGELKGKDLEELIAEGSKKLASVPSGGAVAAAPAGGAAPAGGAAPAEAKKEEKKDES-EES Lumbricusr RYVAAYLLAVLGGNEHPGEADVKKILSSVGIDADNDCVKKVVGELKGKNLEELIAAGRQKMASMPSAS-GAAPSAAAAPAAAAAEPAEAKKEEPKKEEEEEE Daphnia___ RYVAAYLLAALGGNEAPTAENIEKILSSVGIEAEADKLSKVIAELKGKNLQTLMAEGQAKLASMPGGAAPAAVAAAPAGGAAPAKAEEKKKEEKK-EESEEE Litopenaeu RYVAAYCLAALGGKTA-SAKDIEKILDSVGVDCDADEAKKVVSELEGKDLENVIAEGMEKLGSMPGGGAPAAGGAAPAAA-----TAEEKKPEKVEEPEEES Drosophime RYVAAYLLAVLGGKDSPANSDLEKILSSVGVEVDAERLTKVIKELAGKSIDDLIKEGREKLSSMPGGGAVAAADAAPAAAAGGDK-KEAKKEEKK-EESESE Apis______ RYVAAYLLAVLGGKTSPNQNDIEKILSSVGIETDGEKLKKVIAELNGKSIEELISQGMEKLLSMPGGGVAVSADAAPVGGTTAPAEEKKEEKKPAKEESESE Ixodes_s__ RYVAAYLLAAMGGNKDPSAADVEKILGSVGIESDAERVKKVISELKGKSVEDVIAKGKEKLASMPGGGGAAPAAASPAAGGAAAAPKEAAKKEEKKEESEES Hypsibius_ RYLAAYLLVTIGGNANPTAADIEKILSSVGIDADKNRVKLVLEKLKGKNIEATIAEGSKKLASLPAAGPAAAAPAAAAKDAAKPAAKEEPKKKEEKKEEPEE Caenor____ KYLGAYLLATLGGNASPSAQDVLKVLEAGGLDCDMENANSVVDALKGKTISEVIAQGKVKLSSVPSGGS-APAAAAPSGG--AA-PKAE--EKKKEEPKEES Brugia____ KYLAAYLLSTMSGNKSPSAKDIEDVLGSVGLDVDMEDANKVVSALSGKSIDEVITAGLAKVSSVPSDAAVSAIAPVVSATPTDALQAGSKKGETKEGPKEES Xiphinema_ RYVAAYLLAQLGGNNEPSLADVKKILGSVGIDAEDDKAKKVIESLKGKNVEELIEKGKTKLASVPSAAGVAASAAAPAAA-GGAAPAQEKKEEKKKEEKEES Asterina__ RYVAAYLLAVLGGNASPSAKDIKQILETVAVRVEDEQLNKVIKQLSGKNIDEVIAEGQSLLASVPT-GGVAAAAGGGGGG-GEAAGAAEEEEKKKEESEEES Strong____ RYVAAYLLLALGGKPNASAADVKKVLQSVGVEIDDEKLGIVIKELEGKKVKDLIEEGKSKLASMPAGGGGAAPAAGGAAAAGGAA--EEKVEAKKEESEEES Cionai____ RYVAAYLLAALGGNESPSSSDIKNILGSVGIDVDDDKLNMVIKELKGKNLEEVIAAGNEKLASVPSGGGGAVAASGGAAA-ADEAPKE---EAKKEESEEES Molgula___ RYVAAYLLAALGGNANPNAAAIKDILGSVGIDVDDEKLNLVVKELKGKSLPDLIAAGNEKLASVPSGGAVASSGGGAGDAGAADAPKEE---AKKEESEEES Branchiost RYVAAYLLAVLGGNANPSAGDIKKILGSVGIDAEDERLNKVIGELKGKDIEEVMAAGRGKLSSMPSGGGVAAAAGGGAAAGGGAAPAEEKKEEKKEESEEES Eptatretus RYVAAYLLATLGGNSSPTAADIRKILDSVGIETEDDKLTKVINELKGKNVNDVISAGFGKLSSMPAGGAVAASASAGAVAVVEKAPAEEKKEE-KEES-EES Petromyz__ RYVAAYLLATLGGNEQPTAIDIKKILGSVGIEADDSKLSKVISELKGKNVEEVISAGFTKISSVPGGVAVSAGVPGGAAPDTAGAPAEVKKEEKKEES-DES Danio_____ RYVAAYLLAVLGGNTNPSAKDIKNILGSVGIEADDERLNKVVSELNGKDINEVMNAGLSKLASVPAGGAVAVSTASAGGGAPAEAPAEEKKEEKKEES-EES Homos_____ RYVASYLLAALGGNSSPSAKDIKKILDSVGIEADDDRLNKVISELNGKNIEDVIAQGIGKLASVPAGGAVAVSAAPGAAPAAGSAPAEEKKDEKKEES-EES Molgula___ RYVAAYLLAALGGNANPNAAAIKDILGSVGIDVDDEKLNLVVKELKGKSLPDLIAAGNEKLASVPSGGAVASSGGGAGDAGAADAPKEE---AKKEESEEES Branchiost RYVAAYLLAVLGGNANPSAGDIKKILGSVGIDAEDERLNKVIGELKGKDIEEVMAAGRGKLSSMPSGGGVAAAAGGGAAAGGGAAPAEEKKEEKKEESEEES Eptatretus RYVAAYLLATLGGNSSPTAADIRKILDSVGIETEDDKLTKVINELKGKNVNDVISAGFGKLSSMPAGGAVAASASAGAVAVVEKAPAEEKKEE-KEES-EES Petromyz__ RYVAAYLLATLGGNEQPTAIDIKKILGSVGIEADDSKLSKVISELKGKNVEEVISAGFTKISSVPGGVAVSAGVPGGAAPDTAGAPAEVKKEEKKEES-DES Danio_____ RYVAAYLLAVLGGNTNPSAKDIKNILGSVGIEADDERLNKVVSELNGKDINEVMNAGLSKLASVPAGGAVAVSTASAGGGAPAEAPAEEKKEEKKEES-EES Homos_____ RYVASYLLAALGGNSSPSAKDIKKILDSVGIEADDDRLNKVISELNGKNIEDVIAQGIGKLASVPAGGAVAVSAAPGAAPAAGSAPAEEKKDEKKEES-EES Molgula___ RYVAAYLLAALGGNANPNAAAIKDILGSVGIDVDDEKLNLVVKELKGKSLPDLIAAGNEKLASVPSGGAVASSGGGAGDAGAADAPKEE---AKKEESEEES Branchiost RYVAAYLLAVLGGNANPSAGDIKKILGSVGIDAEDERLNKVIGELKGKDIEEVMAAGRGKLSSMPSGGGVAAAAGGGAAAGGGAAPAEEKKEEKKEESEEES Eptatretus RYVAAYLLATLGGNSSPTAADIRKILDSVGIETEDDKLTKVINELKGKNVNDVISAGFGKLSSMPAGGAVAASASAGAVAVVEKAPAEEKKEE-KEES-EES Molgula___ RYVAAYLLAALGGNANPNAAAIKDILGSVGIDVDDEKLNLVVKELKGKSLPDLIAAGNEKLASVPSGGAVASSGGGAGDAGAADAPKEE---AKKEESEEES Branchiost RYVAAYLLAVLGGNANPSAGDIKKILGSVGIDAEDERLNKVIGELKGKDIEEVMAAGRGKLSSMPSGGGVAAAAGGGAAAGGGAAPAEEKKEEKKEESEEES Eptatretus RYVAAYLLATLGGNSSPTAADIRKILDSVGIETEDDKLTKVINELKGKNVNDVISAGFGKLSSMPAGGAVAASASAGAVAVVEKAPAEEKKEE-KEES-EES Petromyz__ RYVAAYLLATLGGNEQPTAIDIKKILGSVGIEADDSKLSKVISELKGKNVEEVISAGFTKISSVPGGVAVSAGVPGGAAPDTAGAPAEVKKEEKKEES-DES Danio_____ RYVAAYLLAVLGGNTNPSAKDIKNILGSVGIEADDERLNKVVSELNGKDINEVMNAGLSKLASVPAGGAVAVSTASAGGGAPAEAPAEEKKEEKKEES-EES Homos_____ RYVASYLLAALGGNSSPSAKDIKKILDSVGIEADDDRLNKVISELNGKNIEDVIAQGIGKLASVPAGGAVAVSAAPGAAPAAGSAPAEEKKDEKKEES-EES

Slide9: 

Results 94 genes 15089 a.a. 16 species 50 genes 8832 a.a. 49 species Automatic searching of synapomorphies in a.a. sequences Synapomorphy in single gene

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Analysis of 94 genes 0,1 65 100 100 100 100 100 50 100 100 100 100 100 100

Results of ribosomal S30 gene analysis: 

Results of ribosomal S30 gene analysis sy synapomorphy

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Conclusions: 1. Two independent analysis (maximum likelihood analysis and synapomorphy in RibS30 gene) make Ichthyosporea group (Amoebidium, Capsaspora, Sphaeroforma) as a sister to Choanoflagellida and Metazoa phyla. 2. Most phylogenetic analysis of concatenated alignments put phylum Ctenophora into the base of animal tree. 3. Bilateria taxons wasn`t clear resolved on different phylogenetic trees. Tree topology depends on taxon and gene sampling. 3