Slide1: Investigation on seed’s growth BEAN HOUSE T.W.G.Hs Mrs. Fung Wong Fung Ting College Kelvin Cheung Kyle Hui Ivy Ma Tracy So


Content: Content Introduction Objectives Materials and Methods Results and Discussions Conclusion


Slide3: Introduction Objects of Study: Green and Red bean  


Slide4: Objectives To investigate : -- the growth and respiration rate patterns -- the relationship between respiration rate and 1) fresh mass 2) dry mass 3) Energy content 4) Heat Production 5) Length of stem, leaf and root of red bean and green bean


Hypothesis: Hypothesis Respiration rate increases as the seeds grow Positive correlation between respiration rate, heat production rate, fresh mass and energy content Negative correlation between respiration rate and dry mass


Materials and Methods: Materials and Methods


Slide7: Trial In trial, same measurements of varies beans Trial shown: common features of red and green bean -do not rot easily -belong to same species -have different growth patterns Modification -- only focus on Red and Green bean -- 3 replicates instead of 1 (except dry mass & energy content)


Slide8: Cultivation of Green Beans 1)Beans are soaked 1 day stimulating germination 2)renew water and sterilize beans(1% Cl water) 3)same environmental conditions


Slide9: Green Bean Phaseolus radiatus Red Bean Phaseolus angularis


Slide10: Experimental Setup – Respiration Rate interface Low pressure sensor Containing 200 germinating bean Cover with aluminum foil (no photosynthesis ) KOH (absorbCO2)


Slide11: Experimental Setup – Heat Production Temperature sensor Sterilized Vacuum flask( containing beans)


Slide12: Length measurement ( plumule, radical and leaf) ruler string


Slide13: Fresh mass and dry mass electronic balance (set zero before use) Aluminum foil dish containing beans


Slide14: Aluminum foil dishes containing beans oven


Slide15: Energy content (Calorimeter in CityU)


Results and Discussions: Results and Discussions


Slide17: Growth Patterns of Beans – Lengths Green Bean Red Bean


Slide18: If correlation index of x and y tends to 1 -- positive relationship ( x while y ) If correlation index of x and y tends –1 -- negative relationship ( x while y ) Correlation Index used to study the relationship between respiration rate and others R2 is used to show whether there is a relationship between two parameters If R2 tends to 1, there is a relationship


Slide19: The regression and correlation between respiration rate and the others


Slide20: Average Respiration Rate increasing rate can provide more energy for growth Time(day)


Slide21: Average Fresh Mass of Beans Time(day)


Regression between respiration rate and fresh mass of red bean (correlation: 0.605): Regression between respiration rate and fresh mass of red bean (correlation: 0.605) respiration rate had a positive relation to fresh mass


Regression between respiration rate and fresh mass of red bean (correlation: 0.605): more cells carrying out division Regression between respiration rate and fresh mass of red bean (correlation: 0.605)


Regression between respiration rate and fresh mass of red bean (correlation: 0.605): increase respiration rate to support high metabolic rate in germination Regression between respiration rate and fresh mass of red bean (correlation: 0.605)


Regression between respiration rate and fresh mass of red bean (correlation: 0.605): high respiration rate of red bean to provide energy for root to absorb mineral salt actively Regression between respiration rate and fresh mass of red bean (correlation: 0.605)


Regression between respiration rate and fresh mass of red bean (correlation: 0.605): lower osmotic potential in it and more water absorbed for cell enlargement Regression between respiration rate and fresh mass of red bean (correlation: 0.605)


Regression between respiration rate and fresh mass of Green bean (correlation:-0.824): Regression between respiration rate and fresh mass of Green bean (correlation:-0.824)


Slide28: Dry Mass of Red and Green Bean


Slide29: a negative relation between respiration rate and dry mass Regression between respiration rate and dry mass of red bean (correlation: -0.805)


Slide30: no photosynthesis in germination, thus no food produced Regression between respiration rate and dry mass of red bean (correlation: -0.805)


Slide31: food reserve is used in respiration to release energy Regression between respiration rate and dry mass of red bean (correlation: -0.805)


Slide32: Regression between respiration rate and dry mass of Green bean (correlation:0.837)


Conclusion: Conclusion Growth Pattern for Green Bean, it develops its roots and leaves first, the increasing rates is higher for Red Bean, it develops its stem first, the increasing rate is higher


Correlation: Correlation


Slide35: Difficulties in the experiment Water supply should be average Young shoots and stems are susceptible to fungal attack Temperature should be appropriate


Slide36: Suggestions for further investigations Green beans have abnormal respiration pattern --investigation on the growth pattern are suggested Longer experimental period If seeds are cultivated in soil, must not be sowed


Acknowledgements: Supervisors : Dr. Richard Cheung Dr. S.G. Cheung Teachers : Miss M.T. Mak Miss K.M. Ng Technicians : Raymond Chan Simon Chow Student Mentor: Chan Wai San Acknowledgements


THE END: THE END


Q & A : Q & A