Oligomerization of the Dopamine Transporter: cocaine-analog-induced conformational changes at a homodimer interfaceJonathan A. Javitch, MD,PhDColumbia University : Oligomerization of the Dopamine Transporter: cocaine-analog-induced conformational changes at a homodimer interface Jonathan A. Javitch, MD,PhD Columbia University


Slide2: The Dopamine Transporter (DAT) is responsible for re-uptake of dopamine from the synaptic cleft Amph


Slide3: Although the inhibition of DAT by cocaine and amphetamine is widely documented, the structural basis of DA transport and its inhibition by cocaine and other psychostimulants such as amphetamine is poorly understood. Accessibility of endogenous cysteines and DAT topology Conformational changes associated with substrate transport and inhibitor binding to DAT Oligomerization of DAT Trafficking of DAT induced by amphetamine, cocaine, and manipulation of signal transduction pathways Mechanism of amphetamine-mediated DA efflux Bacterial and archaeal sodium-dependent transporters as model systems for structural studies


Slide4: Oligomerization of neurotransmitter transporters?? (The horror! The horror!) Serotonin transporter: Co-IP effects of cysteine modification FRET GABA transporter FRET Glycine transporter: dimer intracellular and not on surface DAT: Radiation inactivation consistent with dimer and/or tetramer FRET


Flag-HA-synDAT: Flag-HA-synDAT


Cross-linking of Flag-HA-DAT: Cross-linking of Flag-HA-DAT


Slide7: DAT runs as a broad band ~85k Da DAT is cross-linked by copper phenanthroline to a broad band ~195k Da DAT is cross-linked by bis-MTSEA to a broad band ~195k Da Cross-linking is reversed by reduction with DTT Are we cross-linking a DAT homodimer or a heterodimer between DAT and another protein?


Myc-His-DAT: Myc-His-DAT


Coimmunoprecipitation of Flag-HA-DAT and Myc-His-DAT: Coimmunoprecipitation of Flag-HA-DAT and Myc-His-DAT


DAT is cross-linked in mouse striatal membranes: DAT is cross-linked in mouse striatal membranes


Which cysteine is responsible? MTSET blocks cross-linking so…: Which cysteine is responsible? MTSET blocks cross-linking so…


Cys306 is necessary for DAT cross-linking: Cys306 is necessary for DAT cross-linking


Flag-HA-CD-DAT: Flag-HA-CD-DAT


Cys306 is sufficient for DAT cross-linking: Cys306 is sufficient for DAT cross-linking


The G(V/I)XXG(V/I)XX(A/T) motif is conserved in TM6 of neurotransmitter transporters: The G(V/I)XXG(V/I)XX(A/T) motif is conserved in TM6 of neurotransmitter transporters


TM6: GVXXGVXXA/T Dimerization motif Deviation from helical periodicity TM6


Mutation of Gly323 and Gly327 abolished uptake: Mutation of Gly323 and Gly327 abolished uptake


Summary of Dimerization: Summary of Dimerization DAT in the plasma membrane can be cross-linked into a dimer by bis-EA or CuP. Cys-306 at the extracellular end of TM6 is necessary and sufficient for cross-linking. DAT is a symmetrical dimer (at least). Cross-linking does not alter uptake or binding. The GVXXGCXXA motif is presumably involved in dimerization of TM6. 'Only' 24 TMs to sort out…. But wait!


DAT is a tetramer in the membrane: a second symmetrical interface in TM4 is crosslinked by Cu++ or HgCl2: DAT is a tetramer in the membrane: a second symmetrical interface in TM4 is crosslinked by Cu++ or HgCl2


Slide20: Cocaine analogs block crosslinking of the TM4 interface but not the TM6 interface – conformational change


Slide21:


Targeting and trafficking of the dopamine transporter: Targeting and trafficking of the dopamine transporter Activation of PKC leads to acute ’downregulation’ of DAT


DAT internalization upon direct PKC activation and upon activation of a co-expressed SP receptor: DAT internalization upon direct PKC activation and upon activation of a co-expressed SP receptor Control 200 nM SP 1 mM PMA HEK-293 cells co-expressing EGFP-hDAT and the substance P receptor (NK-1)


The hDAT contains multiple putative Ser/Thr phosphorylation sites: The hDAT contains multiple putative Ser/Thr phosphorylation sites PKC consensus sites Other putative internalization motifs Non-consensus sites


No effect of mutating multiple serines and threonines in different combinations: Surface biotinylation Uptake No effect of mutating multiple serines and threonines in different combinations


Direct protein kinase C activation and activation of a co-expressed SP receptor increase phosphorylation of DAT: Control FLAG-hDAT 0 SP PMA 0 SP PMA 105 kD 0 SP PMA FLAG-HA-hDAT- D1-22 Direct protein kinase C activation and activation of a co-expressed SP receptor increase phosphorylation of DAT Western blot Phosphorylation


Phosphorylation of a non-DAT substrate mediates internalization: Phosphorylation of a non-DAT substrate mediates internalization


Properties of N-terminal truncation mutant: Properties of N-terminal truncation mutant Internalization is normal in response to PMA Internalization is normal in response to SP Surface expression is normal/enhanced Km of dopamine and tyramine are normal Vmax/surface DAT is normal Oligomerization is normal


Slide29: Acknowledgements Charlotta Grånäs Claus Juul Loland Ulrik Gether Habibeh Khoshbouei Aurelio Galli Bipasha Guptaroy L'Aurelle Johnson David Lund, Margaret E. Gnegy Mu Fa Zhou Amy Newman Shonit Das – bacterial transporters Yvette Dehnes – DAT IL3 Jasmine Ferrer – DAT - the beginning Naomi Goldberg - bacterial transporters Wen Guo – receptor Hanne Hastrup – DAT X-linking George Liapakis – receptor Matthias Quick - bacterial transporters Namita Sen – DAT regulation Lei Shi - receptor Merrill Simpson - receptor Mark Sonders - DAT Arthur Karlin NIDA