SherylMaher

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Rapid Detection of Killer Viruses!: 

Rapid Detection of Killer Viruses! Sheryl Maher

Killer Viruses: 

Killer Viruses Ebola Marburg Yellow Fever Japanese Encephalitis Dengue West Nile SARS Avian Influenza Human Immunodeficiency Rabies Hendra Nipah Small Pox Polio

Global Dengue Distribution 2005: 

(Centre for Disease Control, 2006) Global Dengue Distribution 2005

How many people are affected?: 

How many people are affected? Yellow Fever 200,000 Cases 30,000 Deaths Dengue 100,000,000 Cases 500,000 DHF 25,000 Deaths Japanese Encepahlitis (Asia) 50,000 Cases 10,000 Deaths and 8,000 Disabilities

Who is affected?: 

Who is affected? (Centre for Disease Control, 2002)

Viruses in Australia: 

Viruses in Australia Murray Valley Encephalitis Kunjin Gadgets Gully Samarez Reef Japanese Encephalitis Dengue 1-4 Alfuy Kokoberra 5 2 2 218

Fever in world traveler! What could it be?: 

Fever in world traveler! What could it be? Case Study

Travel Plan: 

Travel Plan

Getting tested: 

Getting tested

Current testing: 

Current testing Patient Sample Cells Die ? Virus infection Nothing happens 3-10 days

Current testing: 

Current testing Virus coated plate Virus Patient Sample Detects patient sample and produces colour Patient Sample

Reading results: 

Reading results

The future of testing: 

The future of testing

A New Way to Test: 

A New Way to Test Only requires one blood sample Patient Sample Biotin Labeled Primer Original DNA Synthesized DNA 3 hours - + Sample

Hybridisation: 

Surface Yes No Yes No Yes No Yes No Hybridisation Probe 1 2 3 4

Virus Identification: 

Virus Identification Dengue 2 Dengue 3 Dengue 4 Gadgets Gully Yokose Samarez Reef Langat Rio Bravo Dengue 1 Kamiti River Yellow Fever Japanese Encephalitis West Nile Murray Valley Encephalitis Kunjin 3 2 1 4

Case study summary: 

Case study summary Diagnosis Dengue Virus Serotype 1 Follow up Warn public (especially local backpacker hotels) about risk of mosquito bites Mosquito control program

Rapid detection: 

Rapid detection Simple test Don’t require follow up samples Rapid results Allows appropriate treatment Preliminary result in 3 hours Range of results Can readily identify ‘unusual’ or emerging pathogens

Acknowledgments: 

Acknowledgments Ross Barnard Roy Hall Elena Virtue Hall and Barnard groups Corinna Lange Stephen Prowse Mark Gibbs Adrian Gibbs Gibbs group Naomi Forrester Ernest Gould Gritsun group