Slide2:
Preparation of the sample Vegetable 50g Acetone 75 ml + blender Wash with Acetone 10 ml filter
Slide3:
+ chloroform 50 ml
+ saturated NaCl 5 ml
+ distilled water to 250 ml Shake 5 min
Slide4:
Re-extract the aqueous phase with chloroform 25 ml Wash the chloroform phase with 100 ml H2O, twice Add sodium sulphate anhydrous 1-2 g filter Evaporate at 700C Re-dissolve in 2 ml acetone
Slide5:
Preparation of standard Selvin 3 g + Chloroform 20 ml filter Wash with chloroform 10 ml evaporate Left about 5 ml
Slide6:
Stationary phase: TLC sheet: silica gel 0.2 mm
Slide7:
2.5 cm 7.0 cm
Slide8:
Apply sample
Slide9:
Put it in the beaker containing mobile phase
Slide11:
Visible bands
Slide14:
Distance moved by the solute Distance moved by the solvent