researchoverviewbiol 211

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Student Projects Cryopreservation Non-structural proteins of the Aedes aegypti densonucleosis virus Medicinal attributes of Chilopsis, the Desert Willow Oligocene turtles

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Practical aspects of Cryopreserving of Cultured Mammalian and insect cells Cryopreservation Studies

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Methods Article Serum-Free Cryopreservation of Five Mammalian Cell Lines in Either a Pelleted or Suspended State Joe Corsini(1)*, Christy Hacker(1), and Charles Bare(2) 1 Biology Department, Chadron State College. Chadron, NE 69337. USA. 2 Mathematics Department, Chadron State College. Chadron, NE 69337. USA. Biol. Proced. Online 2004;6:61-66. doi:10.1251/bpo73 Submitted: November 05, 2003; Accepted: March 16, 2004; Published: April 07, 2004.

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Figure Enlarged Fig. 1:

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© 2005 by the author(s). This paper is Open Access and is published in Biological Procedures Online under license from the author(s). Copying, printing, redistribution and storage permitted. Journal © 1997-2005 Biological Procedures Online. Biol. Proced. Online 2005; 7(1): 26-30. doi:10.1251/bpo102 March 14, 2005 Rapid cryopreservation of five mammalian and one mosquito cell line at-80°C while attached to flasks in a serum free cryopreservative Joe Corsini1* and Ethan Mann1 1Chadron State College, Chadron, NE 69337. *Corresponding Author: Joe Corsini, 11490 Pine St., Sandpoint, ID 83864. Phone: (208) 263-7055; Email: joe_corsini@hotmail.com Submitted: January 3, 2005; Revised: February 11, 2005; Accepted: February 17, 2005. Indexing terms: Cryopreservation, Serum-free, Cultured cells. © 2005 by the author(s). This paper is Open Access and is published in Biological Procedures Online under license from the author(s). Copying, printing, redistribution and storage permitted. Journal © 1997-2005 Biol. Proced. Online 2005; 7(1): 26-30. doi:10.1251/bpo102 March 14, 2005 Rapid cryopreservation of five mammalian and one mosquito cell line at-80°C while attached to flasks in a serum free cryopreservative Joe Corsini1* and Ethan Mann1 1Chadron State College, Chadron, NE 69337. *Corresponding Author: Joe Corsini, 11490 Pine St., Sandpoint, ID 83864. Phone: (208) 263-7055; Email: joe_corsini@hotmail.com Submitted: January 3, 2005; Revised: February 11, 2005; Accepted: February 17, 2005. Indexing terms: Cryopreservation, Serum-free, Cultured cells.

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Malaria? Dengue?West Nile?La Crosse? Yellow Fever?Filariasis? x Malaria? Dengue?West Nile? La Crosse? Yellow Fever? Filariasis?

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Characterization of Aedes aegypti densovirus non-structural proteins 1)Non-enveloped, Icosohedral virus 2)Single-strand DNA, Infectious clone available,sequenced 3)Two non-structural, two structural proteins 4)Infects numerous mosquito species

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Parvovirus AeDNV a)Densonucleosis virus (parvovirus) b)Kills a variety of other mosquito species but not undergraduate students c)Grows in mosquito cell culture d)Licensed as insecticide in the Ukraine FEED ME!

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PANEL OF ANTIBODIES Anti-NS1 Anti NS2 Anti VP1 Anti VP1/2

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Anti-NS1 1ary and 2ary Anti-NS2 1ary and 2ary 2ary alone Ethan Mann and Eddie Moran…….

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NS1-GFP NS1-GFP only antiNS1 only

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Summary of Antibody Work 1)Anti-NS1 and NS1-GFP yield the same localization patterns. 2)NS1 and NS2 co-localize in the infected mosquito cell. NS2 also occurs in the cytoplasm. 3)Anti-NS1 and anti-NS2 antibodies specifically detect viral Non-structural proteins. In addition, there appear to be many variants of NS1 and NS2

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PARVOVIRUS MINUTE VIRUS OF MICE CONFORMATIONAL CHANGES IN THE DNA BINDING PROTEIN UPON BINDING TO DNA? QUESTION

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672 amino acids

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viral peptide viral DNA interact not interact conformational change no conformational change

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Cytotoxicity bacteria, yeast, and cultured mammalian cells Chilopsis, the Desert Willow, used medicinally by the Apache tribes

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A B C D MCF7 Human breast

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Taphonomical studies of Oligocene Turtles Paleoecology and Evolution

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FOSSIL TURTLES OF NEBRASKA’S WHITE RIVER GROUP: A BIAS TOWARD THE CARAPACE UP POSITION Society for Vertebrate Paleontology Conference 2003. Joe Corsini, Amanda Dopheide, and Mike Leite Chadron State College, Chadron, NE 69337

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# Carapace up/ total carapace determinations Meters from Chamb. Pass Table 1 – Ratio of carapace up turtles vs total number of carapace position determinations at 10 meter intervals.

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Large Chadronian tortoise, collected near Toadstool park campground.

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Thanks: Grady Costello, Amanda Dopheide, & Nebraska’s EPSCOR Grant