Antibacterial activity of ethanolic leaf extract of Kalanchoe pinnata

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B.Deepthi et al / Int. J. of Allied Med. Sci. and Clin. Research Vol-34 2015 555-558 555 IJAMSCR |Volume 3 | Issue 4 | Oct – Dec - 2015 www.ijamscr.com Research article Medical research Antibacterial activity of ethanolic leaf extract of Kalanchoe pinnata and its application in the treatment of abscess caused by staphylococcus aureus B.Deepthi K.Shivakanth Reddy Y.Keerthi Reddy L.Sushma A.Shambashiva Rao Department of medicinal chemistry Sri Indu Institute of Pharmacy Sheriguda Hyderabad. Corresponding author: B.Deepthi ABSTRACT In this study we have found anti-bacterial activity of ethanolic extract of leaves of Kalanchoe pinnata against microbial flora organism staphylococcus aureus which was measured as parameter of activity which was compared against std. drug amoxicillin antibiotic. The plant extract shows potent zone of inhibition than the std. compound. Leaf extract of chloroform water showed mild zone of inhibition compared to that of ethanolic extract. Aim of study was to extract the constituents from leaves of Kalanchoe pinnata to explore anti-bacterial activity against staphylococcus aureus using disc diffusion method with the obtained results we have concluded that the ethanolic extract can be used in treatment of abscesses because of its potency against staphylococcus aureus. Keywords: Kalanchoe pinnata Staphylococcus aureus Anti-Bacterial activity. INTRODUCTION Kalanchoe pinnata is also known as bryophyllum pinnatum miracle leaf life leaf life plant. Distinctive for the plantlets that form on margin of its leaves Chemical constituent found in this plant are alkaloids arachidonic acid bufadienolides and astragalin cinnamic acid and clevosterol and bryophillin bryophyllol. Etc. Leaf extracts of Kalanchoe pinnata are used along with coconut oil in the treatment of burns and skin ulcer. ethanolic methanolic extracts of leaves were used in the treatment of diabetic skin diseases and aqueous extracts were used as anti-inflammatory anti- diabetic and anti-tumour agents. As a part of our study we studied anti-bacterial activity of ethanolic extract of leaves of Kalanchoe pinnata and applied in the treatment of abscess in albino rat. MATERIAL AND METHOD Plant collection - local area nursery Hyderabad Plant part used - leaves Chemical used - Aqueous Chloroform Ethanol Apparatus used - Soxhlet apparatus reflux condensor. PLANT MATERIAL We have collected the plant from rural area and then washed the plant with water. EXTRACTION Extraction with aqueous water The fresh leaves were cut to reduce size and dried in sun light which is subjected to soxhlation is extracted with water for 6 hours. Extract is collected and dried in hot air oven at600c in order to obtain solvent free extract dried content which is stored in closed container. ISSN: 2347-6567 International Journal of Allied Medical Sciences and Clinical Research IJAMSCR

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B.Deepthi et al / Int. J. of Allied Med. Sci. and Clin. Research Vol-34 2015 555-558 556 Extraction with chloroform The fresh leaves were cut to reduce size and dried in sun light which is subjected to soxhlation is extracted with chloroform for 6 hours. The extract is collected in a china dish dried in hot air oven at 40-500c in order to obtain solvent free extract. Stand the dried extract in closed container. Extraction with ethanol The fresh leaves were cut to reduce size and is subjected to soxhlation is extracted with ethanol for 1 hours. Collect the extract in a watch glass and evaporate the solvent in hot-air oven at 40-500C to obtained dried content. Stand the dried extract in closed container. PHYTOCHEMICAL EVALUATION Phytochemical test for the quantitative presence of alkaloids tannins glycosides carboxylic acids groups were measured by methods described below Test for alkaloids Wagners test - Alkaloids give a reddish brown precipitate with Wagners reagent solution of iodine in potassium iodide. Test for tannins Ferric chloride test - test solution gives blue green colour with ferric chloride. Test for carboxylic acid Sprinkle small amount of leaf extract on an aqueous solution of sodium bicarbonate shows the effervescence which indicates of presence of acids. ANTIBACTERIAL ACTIVITY Disc diffusion method Preparation of nutrient medium wells are created. Transfer of microbial suspension by pour plate method. Using a flame-sterilized forceps gently press each disc to the agar to ensure that the disc is attached to the agar. Plates should be incubated overnight at incubation at temperature of 37 °C. After a day the plates are removed and measured for zone of inhibition Chloroform extract. Chloroform extract Ethanolic extract Standard drug IN-VIVO TEST Application of ethanolic extract on albino rat for activity on abscess Organism sample was introduced through intrathecal route to create an abscess. After 24 hours the ethanolic extract was applied topically on the skin of the rat which prevented the formation of abscess internally and. This ethanolic extract of Kalanchoe pinnata was able to prevent the formation of abscess. When applied on the surface with incession which was already infected with staphylococcus aureus organism.

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B.Deepthi et al / Int. J. of Allied Med. Sci. and Clin. Research Vol-34 2015 555-558 557 Pics for prevention against formation of abscess i.e. ethanolic extract activity Prevention of internal abscess Prevention of abscess in incision infected with organism staphylococcus aureus RESULTS Microbial assay In-vivo test S.no. Organism Zone of inhibition 1 Staphylococcus aureus standard Chloroform extract Aqueous extract Ethanolic extract 7.5mm 2mm Nil 18mm Ethanolic extract of Kalanchoe pinnata was able to prevent the formation of abscess both internal external when applied topically to Albino rat. DISCUSSION Following the standard extraction was performed in leaves of Kalanchoe pinnata using various solvents from which ethanol was tested against staphylococcus aureus sample which was compared with standard drug and was proved to be more potent antibacterial agent. All the plant constituents were tested using reagents and for its activity against treatment of abscess were tested on an albino rat. CONCLUSION Present study on the leaves of kalonchoe pinnata i.e. ethanolic extract showed maximum inhibitory effect on organism staphylococcus aureus and was also applied for the treatment of abscess both internal and external and further studies should be conducted in order to perform the clinical trials. ACKNOWLEDGEMENT The author thanks to the Department of Botany of Osmania University and also to Sri Indu Institute Of Pharmacy for providing all the facilities related to our work.

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B.Deepthi et al / Int. J. of Allied Med. Sci. and Clin. Research Vol-34 2015 555-558 558 REFERENCES 1. Baldwin jr. T. kalanchoe: the genus and its chromosomes. American journal of Botany. 258: 572-5791938 2. K. Gandhi and R. Guptha. Alkenes Akanols Triterpenes and Sterols of Kalanchoe pinnata. Phytochemical. 11:1500-15021972 3. Lans CA. Ethno medicines used in Trinidad and Tobago for urinary problems and diabetes mellitus. Journal Ethnobiol Ethnomed. 245:1745-17502006 4. Ghani medicinal plants of Bangladesh The Asiatic Society of Bangladesh Dhaka Second Edition 3822003 5. E. A. Cruz SAG DA-Silva MF. Muzitano PMR Silva SS Costa B Rossi Bergmann Immuno modulatory pre-treatment with Kalanchoe pinnata extract its protects mice against flatal anaphylactic shock. International immuno pharmacology. 81616-16212008 6. Vinit Raj Arivind Kumar Vaishal Singh Praveen Kumar Vinod Kumar. In Vitro Antimicrobial Activity of Kalanchoe pinnata Leaf vol:4 issue-32012 7. Shazid M Mohammad K. Hossain Mohammad R. Haque Abu A. Chowadhury Md. A. Kaisar Choudhury M. Hasan Mohammad A. Rashid Chemical and Biological Studies of Kalanchoe pinnata Lam. Growing in Bangladesh2012 8. Siddhartha PA Chandhuri KW Anti-inflammatory action of Bryophyllum pinnatum leaf Extract. Fitoterapia 1990 41: 527-533 9. Kaisari MA Rahman MS Hasan CM Rashid MA A Review on Phytochemical from some Medicinal Plants of Bangladesh. J Pharm Nutri Sci 2011 1: 87-95 10. Yadava RN Chakarvarthi N. New Antifungal Triterpenoids Saponin from Launaea pinnatifida Cass. Indian J Chem 2009 48: 83-87 11. Brand-Williams W Cuvelier ME Berset c. Use of a Free Radical Method to Evaluate Antioxidant Activity. Food Sci Tech Lebensm Wiss Technol 1995 28: 25-30 12. Bauer AW Kirby WMM Sherris JC Turck M. Antibitic Suscepitibility testing by a standardized single Disc method. Am J Clin Pathol 1996 45 :493-496 13. Almey AAA Khan CAJ Zahir IS Suleiman KM Asiayah MR Rahim KK. Total Phenolic Content and Primary Antioxident Activity of Methonolic and Ethanolic Extracts of Aromatic Plant Leaves. Int Food Res J 2010 17: 1077-1084 14. Pateh UU Haruna AK Garba M Iliya I Sule Im Abubakar MS etal Phytochemical Screening and Histopathological studies on the Seeds of colocynthis Citrullus in Albino rats. Nig Journ Pharm Sci 2008 7: 19-25 How to cite this article: B.Deepthi K.Shivakanth Reddy Y.Keerthi Reddy L.Sushma A.Shambashiva rao Antibacterial activity of ethanolic leaf extract of kalanchoe pinnata and its application in the treatment of abscess caused by staphylococcus aureus. Int J of Allied Med Sci and Clin Res 2015 34: 555-558. Source of Support: Nil. Conflict of Interest: None declared.

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